Dissertation > Excellent graduate degree dissertation topics show

Studies on in Vitro Culture of There Kinds of Bovine Somatic Cells

Author: ZhangMin
Tutor: YiJianMing;YangLiGuo
School: Huazhong Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Bovine Skin fibroblast Granulose cell Oviduct epithelial cell Cell culture
CLC: Q813
Type: Master's thesis
Year: 2006
Downloads: 207
Quote: 2
Read: Download Dissertation


There were many factors influenced the success rate of mammal nuclear transfer from preparation of donor cells and oocytes, reconstruction combination of embryos, culture of reconstructed embryos, pregnancy to birth of foetus. In vitro culture of donor cell is one important factor of them. In our studies, the method of separation and culture were investigated on bovine skin fibroblast cell, granulose cell and oviduct epithelia cell in order to establish a steady method of donor cell culture in vitro, and the same time the biological characteristics of somatic cells were studied and discussed. The results as follows:1. DMEM and 1640 are more appropriate to the culture of bovine ear skin in vitro than M199.2. Hot digestion, cold digestion and cold digestion combined hot digestion all can gain many bovine ear skin fibroblast cells, and cold digestion combined hot digestion bring more fibroblast cells and high yield of viable proliferating fibroblast cells than hot digestion and cold digestion.3. Effect of three different culture methods on growth of subculture fibroblast were studied. Digested tissue culture method was better than cell culture method and tissue culture method on gaining higher touch rate of cells, more alive cells and shorter culture time.4. In 37℃, 0. 25%trypsin+0. 02% EDTA was an appropriate digested medium for subculture of bovine granulose cells.5. The rate of chromosome numerical aberration of skin fibroblast cells (passage 0-2), granulose cells (passage 0-4) and oviduct epithelia cells (passage 0-3) were low (less than 10%) in vitro, and those cells can be used as donor cell in nuclear transfer.6. The volume of culture medium has more important effect on the touch rate of primary granulose cell than culture area. 1. 33mL of culture medium in 6-well culture plate was more appropriate to the development of cells than other groups, and there. were no significant difference in touch rate of primary granulose cell between different culture area.

Related Dissertations

  1. Isolation and Cultivation of Rabbit Adipose-derived Stem Cells and the Influence of Platelet-rich Plasma on the Cell Proliferation,R329
  2. Primary Research on Strring Culture and Storing of Human Dermal Fibroblasts,R329
  3. A Simple and Efficient Method That Uses Low Concentration Fetal Bovine Serum to Culture and Purify Schwann Cells,R329
  4. Cloning of Fucal in Monopterus Albus and the Differentiation Expression of SSEA and Fucal in Monopterus Albus Gonad Tissue,S917.4
  5. Morphology and Phylogenetic Analysis on Endoreticulatus Sp. Zhenjiang and Nosema Sp. MPr,S884
  6. Hyaluronic Acid Gel Induction MDSC Differentiation for Chondroid Cells,R329
  7. EPCs Culture from Peripheral Blood in Vitro and the Study of Tongxinluo on Adhesion and Proliferation Activity of EPCs for CHD Patients,R285
  8. Cloning and Expression of Antimicrobial Peptides of Cathelicidin in Bovine Marrow,S858.23
  9. The Effect of Hepatocyte Growth Factor Concentration on Differentiation of Human Bone Marrow Mesenchymal Stem Cells to Cardiomyocyte-like Cells,R329
  10. Mapping B-cell Liner Epitopes of NS3 Protein of Bovine Viral Diarrhea Virus,S852.65
  11. Cryopreservation of the Fibroblast in Black Bear and the Effect on Black Bear-Bovine Cloned Embryos,S865.31
  12. Technology of Bovine Stem Cells,S823
  13. Study on a Technology Realized the Large-Scale Production about the Comprehensive Development and Utilization of Bovine Blood,S823
  14. Recombinant Brucella Abortus S19 Expressing Mycobacterium Tuberculosis Ag85B Antigen,R392.1
  15. Study on Protective Effect and Mechanism of Ginkgolide B on Embryonic Rat Midbrain Neurons,R285.5
  16. The Study of Ginkgo Biloba Extract on the Impact of High-pressure in Vitro Culture of Rat Retinal Ganglion Cell with Apoptosis Skeletal Morphology and Structure,R285.5
  17. The Genetic Characteristics of PAX6 and LHX4 Gene and Their Association with Growth Traits in Cattle,S823
  18. Methodological Study of Piglet Alveolar Epithelial and Vascular Endothelial Progenital Cells,R329
  19. Evaluation of Antimicrobial Resistance and Treatment Failures for Chlamydia Trachomatis,R759
  20. The Construction of Bactericidal Permeablility-Increasing Protein and Defensins Fusion Plasmid of Cattle and Its Expression in E. Coli,S852.5
  21. Preparation and Identification of Monoclonal Antibodies Against E2 and NS3 Protein of BVDV,S852.65

CLC: > Biological Sciences > Bioengineering ( Biotechnology ) > Cell Engineering
© 2012 www.DissertationTopic.Net  Mobile