Dissertation > Excellent graduate degree dissertation topics show

Effect of Different Pathways and Doses of Inoculation on Establishment of Experimental Tuberculosis in SD Rats

Author: YuanXiaoLiang
Tutor: LeiJianPing
School: Nanchang University
Course: Internal Medicine
Keywords: Sprague-Dawley rats Tuberculosis Mycobacterium tuberculosis / microbiology Animal models
CLC: R-332
Type: Master's thesis
Year: 2006
Downloads: 98
Quote: 0
Read: Download Dissertation

Abstract


Objective: To establish a of SD rats tuberculosis model (including the route of inoculation and the amount of inoculum ) , comparing different routes of infection and the amount of inoculum role in the establishment of the SD rat animal model of tuberculosis . Subjects and Methods: 70 SD rats were randomly divided into normal control group (A ) tail vein the 0.1mg infection group (B ) , the tail vein 1mg infected group (C ) , the tail vein 10mg infected group ( D group ) , intra-abdominal of 0.1mg infection group (E ) , intra-abdominal a 1mg infection group (F ) and the abdominal cavity 10mg infected group (G group ) , 10 in each group . Standard human strains of TB H 37 Rv0.1mg , 1mg 10mg three inoculant respectively injected via the tail vein and intraperitoneal two ways SD rats . At the same time , the control group were injected with the same volume of saline as a control. Were killed and six weeks after rats were weighed and calculated comparing lung , organ mass index of liver, spleen , lung , liver , spleen general lesions and microscopic characteristics , lung , liver, and spleen tissue homogenates smear done acid-fast staining and homogenate cultures observe whether mycobacterial colony growth . Results: 1 . Tail vein 1mg infection group , lung weight index , the tail vein 10mg infection group and the normal control group was significantly increased ( P <0.05 ) . The tail vein 10mg infection group , intraperitoneal 10mg infections in liver weight index was significantly higher than the control group ( P <0.05 ) . The tail vein of 1mg infection group , the tail vein 10mg infection group , abdominal 10mg infection spleen weight index and the normal control group significantly increased ( P <0.05 ) . 2. The tail vein 10mg infected lung , liver , spleen weight index with celiac 10mg infection group lung , liver , spleen weight index , there were significantly increased ( P <0.05 ) . 3. HE staining of lung , liver , spleen , see TB nodules varying degrees -added performance that lesions epithelioid cells and macrophages gathered into a group , surrounded by lymphocytic infiltration . With the increase in the inoculum size , the pathological changes of the organs and tissues in the more significant . Inoculant consistent tail vein infection in lung , liver , spleen pathological changes significantly more abdominal infection .

Related Dissertations

  1. Study on the Correlation of murA Gene and Mycobacterial Growth,Q78
  2. The Relation of the Height of the Remnants of Thoracolumbar Tuberculosis and Selection of Mode of Anterior Internal Fixation,R687.3
  3. Diagnosis Value of Cytokine Levels and TB-Ab in Serum in Children Tuberculosis and Latent Tuberculosis Infection,R725.1
  4. Experimental Study of Th17 Related Factors of Animal Models Infected with Mycoplasma Suis,S858.28
  5. 7 Cases Brucellosis Misdiagnosed as Spinal Tuberculosis Reason Analysis,R516.7
  6. Study of the Rapid Diagnosis of Mycobacterium Tuberculosis and Drug Resistant Mycobacterium,R440
  7. Combined Use Direct Endotoxin Absorption and High Volume Hemofiltration on the Influence of Endothelial Cells,R459.7
  8. Study on Corneal Neovascularization and Prognosis of Fungal Keratitis,R772.21
  9. Diffusion-weighted Imaging and Apoptosis Research in Rabbit Models of Hepatic VX2 Carcinoma After Radiotherapy,R735.7
  10. Different Frequencies of Electroacupuncture Pretreatment on Rabbit Model of Acute Myocardial Ischemia Comparison of Protective Effect,R245
  11. QSAR Studies on Medicinal Activity Based on Quantum Chemical Calculations,TQ460.1
  12. Study of the Antituberculosis Drug-induced Hepatotoxicity and the IFN-γ、IL-10 Levels in Such Patients,R595.3
  13. The Research of Canine Femoral Head Necrosis Model Constructing by Endovascular Technique,R-332
  14. The Correlation Analysis for the Mode of Obstructive Atelectasis in MSCT and Pathology,R-332
  15. Meta-analysis on the Association Between the Polymorphism of VDR、NRAMP1 and IL-10 and Tuberculosis Susceptibility,R52
  16. Screening of Chinese Old World Monkey TRIMCyp Gene and Construction of Pseudotyped Human Immuno Defieiency Virus-l,Q93
  17. The Study on Mycobacterium Tuberculosis Genotyping and Drug Resistance and Regional Distribution Relationship in Henan,R440
  18. PCR-LDR- nucleic acid test strip assay in Mycobacterium tuberculosis katG gene 315 codon mutation detection,R440
  19. Generation of Reference Standards and Development of a Novel Method for Detection of Culture Filtrate Protein 10 of Mycobacterium Tuberculosis,R378
  20. A Study on the Association of the Polymorphisms of MBL Genes with Susceptibility to Tuberculosis in Xinjiang Hans,R52
  21. The Sociological and Economic Evaluation and Prediction of TB Control Project in Mongolia,R52

CLC: > Medicine, health > Medical research methods > Experimental medicine, medical experiments > Medical Laboratory Animal Science
© 2012 www.DissertationTopic.Net  Mobile