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The purpose of the establishment of the Department of SMMC-7721 liver cancer of Balb / c nude mice xenograft model to study low-frequency ultrasound radiation microbubble agent SMMC-7721 in nude mice transplanted tumor volume growth inhibition the circumstances and tumor histopathological changes observed tumor SOD concentration, CD105 expression number of situations and MVD to study the correlation between the growth rate of the tumor volume and SOD, MVD. And to explore the possible mechanisms that produce the results. Adjust the concentration of SMMC-7721 hepatoma cells to 5 × 106/ml, each left of nude mice subcutaneous injection of 0.2 ml to establish the Department of SMMC-7721 liver cancer Balb / c nude mouse model; model was successfully established after 50 bare The rats were randomly divided into five groups, the control group (A), microbubbles alone group (B), a simple ultrasound group (C), the the ultrasonic radiation microbubbles every other day group (D), ultrasonic radiation microbubbles daily group (E), each The group of 10 model in nude mice. Each group using the appropriate processing method for a week measuring tumor size, tumor volume was calculated growth rate and draw the curve of tumor volume growth; take the tumor specimens, paraffin section, HE staining was observed under the electron microscope the tumor tissue disease Science injury; application of immunohistochemistry analysis of the local oxygen radical metabolites SOD concentration; analysis of the correlation between the growth rate and SOD concentration. Same immunohistochemical method used to observe the differences in CD105 expression in the tumor, and thus counted for each group of tumor microvessels (MVD), and analysis of the correlation between the MVD and tumor volume growth rate. Results of the second week of the SMMC-7721 hepatoma cells after planting can be touched in Balb / c nude mice bearing subcutaneous tumor nodules. Tumor volume after the corresponding treatment after a week, between the groups, the different size of the tumor volume (cm3): A group (0.207 ± 0.081), B group (0.216 ± 0.019), C group (0.180 ± 0.015) in group A than in group D ( 0.139 ± 0.029) and Group E (0.122 ± 0.025) significantly increased, (P lt; 0.05); between groups A, B, C, D, E group no statistical difference. Tumor volume growth rate (%): D group (73.20 ± 0.138), E group (57.24 ± 0.1653), tumor volume growth rate is significantly lower than the control group A (121.24 ± 0.347), B group (123.13 ± 0.232) , C group (108.74 ± 0.221), (P lt; 0.01). D and E groups had no significant difference between A, B and C groups was not statistically significant. Pathological examination see D, E group within the tumor specimens showed obvious thrombosis and large areas of infarction. Immunohistochemical method to get the local SOD-positive rate (%): Group D (28.83 ± 2.483), E group (32.50 ± 3.017) were higher than in group A (11.50 ± 2.881) (P lt; 0.01) while the A, B, no statistical difference between the groups C, D, E group. And a negative correlation between the SOD-positive rate and the rate of tumor growth, (r = -0.663). The CD105 immunohistochemistry results showed that positive staining of the A group, B group and C group increased significantly compared with the Group D and Group E the The MVD count results show: group A (64.6 ± 2.03), B (68.0 ± 1.32) groups, and C ( 59.3 ± 1.21) group D (31.3 ± 1.21) group and E (28.1 ± 0.81) group was significantly increased, D, E, two groups, with group A compared with statistical significance (P lt; 0.01). A group, B group, C group no statistical difference was no statistical difference between group D and group E, a positive correlation (r = 0.576) between the MVD and tumor volume growth rate.
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