Dissertation > Excellent graduate degree dissertation topics show

Study on Separation and Purification of Glycoprotein from Chinese Yam

Author: ShaoHai
Tutor: GongGangMing
School: Shanghai Ocean University,
Course: Applied Chemistry
Keywords: Yam glycoprotein Microwave Technology Purification Molecular weight Antioxidant activity
CLC: TQ914.1
Type: Master's thesis
Year: 2009
Downloads: 163
Quote: 0
Read: Download Dissertation

Abstract


Glycoprotein is formed by a polysaccharide with the polypeptide or protein covalently linked binding protein. The study found that possess immunomodulatory glycoprotein, inhibit tumor, reducing the role of blood sugar, blood lipids, antioxidant, anti-aging, improve people's health and has broad application prospects. This paper yam glycoprotein microwave-assisted extraction, water extraction, purification, molecular weight determination of its antioxidant activity, the main conclusions are summarized as follows: 1. Microwave power in microwave assisted extraction, microwave auxiliary extraction time, liquid material than three factor orthogonal experiment, and the range analysis and variance analysis, and the results are as follows: ① optimum extraction conditions for microwave power 200 W, microwave-assisted extraction time of 1 min, liquid to solid ratio of 10 mL / g. ② affect yam sugar protein was the rate of major factors microwave power, followed by microwave-assisted extraction of time, liquid feed ratio, which microwave power and microwave-assisted extraction of time to yield significant forward while the liquid material than on the very rate there is a certain degree of impact. ③ verify the optimum extraction conditions, the results show that the extraction, under the condition that the average of 1.871% yield of glycoprotein. The water bath temperature water extraction, water extraction time, liquid to solid ratio of three factors orthogonal experiment, and the range analysis and variance analysis, tests showed that: ① The optimum extraction conditions for the water bath temperature 40 ℃, water extraction time was 2 h, the liquid to solid ratio of 10 mL / g. ② to affect glycoprotein yam yield the main factors is the temperature of water bath, followed by liquid feed ratio, water extraction time. Water bath temperature, liquid ratio and water extraction time on the yield is not large. ③ verify the optimum extraction conditions, the results show that the extraction, under the condition that the average of 1.007% yield of glycoprotein. Henan yam glycoproteins purified by chromatography on DEAE-52 and Sephadex G-75 column, obtained the two glycoproteins component GLP-I and GLP-II. Purity identification of the two glycoproteins by polyacrylamide gel electrophoresis and molecular weight determination. The results showed that GLP-Ⅰ and GLP-Ⅱ to electrophoretic homogeneity, and its molecular weight of 13000 Da and 38000 Da. Jiangsu yam glycoproteins purified by chromatography on DEAE-52 and Sephadex G-75 column to obtain a glycoprotein component. Purity identification and molecular weight determination by polyacrylamide gel electrophoresis of the glycoprotein. The results showed that the glycoprotein to electrophoretic homogeneity, and its molecular weight to 35200 Da. 5. Investigated by measuring the clearance rate of the hydroxyl radical and the SOD activity of the antioxidant activity of the glycoprotein yam. The results showed that the The yam glycoprotein scavenging and enhance the role of SOD-like activity, yam glycoprotein certain antioxidant capacity.

Related Dissertations

  1. Study on Protective Effects of Apple Polyphenol on Immune System Injury Caused by γ-Ray,S661.1
  2. Study on Wastewater Purification Effect of the Free Surface Wetland with Different Substrates and Plant Combination,X703
  3. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  4. Studies on Isolation, Purification and Chemical Structure for Polysaccharides from Camellia Chrysantha(Hu) Tuyama,S567.19
  5. Research of Glycosaminoglycan from Perna Virids Effect on Blood Lipid,R285.5
  6. Purification of Endogenous Proteases from (Litopenaeus Vannamei) Shrimp Head and Their Enzymology Properties,S985.21
  7. Study on the Preparation of the Low Molecular Weight Fuciodan from Sargassum Henslowianum (C.Agardh) and Anti-tumor Activity,TS254.9
  8. Under microaerobic conditions closed calcium carbide furnace exhaust COS gas adsorption purification,X781
  9. Studies on Extraction and Analysis of Fructooligosaccharides in Smallanthus Sonchifolius,TS255.1
  10. Experiment Study of the Biogas Purification Process by Zeolite Filling Washing,TQ221.11
  11. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  12. Extraction, Purification and Antifungal Activity of Anthocyanins in Drop Fruits of Chinese Baberry,TQ914.1
  13. Study on the Separation and Purification Way of Arctiin from Fructus Arctii and the Effect of Arctiin on the Growth of Mice C2C12 Cells,S853.7
  14. Purification of Human Respiratory Syncytial Virus Fusion Protein with Immunomagnetic Microsphere Technology,R373
  15. Identification of Marine Sreptomyces Sp. GY-4 and Researches on Its Antimicrobial Substances,Q936
  16. Prokaryotic Expression and Purification of Human Beta-Defensin-9,Q78
  17. The Research of Synthesis of Bisphenol-A Polycarbonate Through Melt Transesterification Process,TQ323.41
  18. Screening of Bacterial Strains with High α-Galactosidase Activity and Its Gene Cloning, Expression, Purification and Characterization,TQ925
  19. Study on Extraction, Isolation and Antioxidant Activity of Lignans of Sesame Cake,TS229
  20. Lipase-catalyzed Transesterification of Lard to Produce L-ascorbyl Fatty Acid Esters,TS221
  21. Study of Isolation and Identification on Antimicrobial Substances Produced by Lactobacillus Acidophilus NX2-6,TS201.3

CLC: > Industrial Technology > Chemical Industry > Other chemical industries > Chemical processing industry of agricultural products > Chemical process and equipment > Chemical processing of agricultural products
© 2012 www.DissertationTopic.Net  Mobile