Dissertation > Excellent graduate degree dissertation topics show

Study on Fermentation Conditions and Extraction Process of Polysaccharide from Liquid Fermentation by Fomes Officinalis Ames

Author: ZhangTao
Tutor: LouZuo
School: Shihezi University
Course: Agricultural Products Processing and Storage
Keywords: Fomes offcinalis Ames polysaccharide fermentation process optimization
CLC: TQ461
Type: Master's thesis
Year: 2009
Downloads: 12
Quote: 0
Read: Download Dissertation

Abstract


The process of liquid fermentation by Fomes offcinalis Ames of Uyghur Traditional Medical to produce polysaccharide were investigated under laboratory conditions. The main research contents and results are as follows:(1) Wild strains of Fomes offcinalis Ames were separated, purified and preserved using the solid plate and slant of rich PDA. At the same time, the culture temperature and time were investigated preliminarily. The results showed that Fomes offcinalis Ames can grow normally at the temperature range of 15-30℃and growth tend to stop after cultivated 8-10 days.(2) In order to obtain the suitable nutrition conditions of seed media, single factor and orthogonal test were carried out for the best choices of sort and concentration of carbon source, nitrogen source, metallic ion and growth factor. The optimal content of seed media obtained according to the yield of biomass were as follows:glucose 3%, peptone 1.5%, KH2PO40.3%, MgSO4·7H2O 0.1%, VB1 0.1 mg/mL. The results showed that the yield of dry mass could reach amount 1.2 mg/mL with the optimized seed media and cultivated 12d.(3) Fermentation media was chose and components of fermentation were optimized by single factor and orthogonal test. The results showed that, cultivated 12d, the yield of dry mass and extracellular polysaccharide could reach amount 1.31 mg/mL and 1.5 mg/mL respectively with the optimized fermentation media that glucose 1%, malt extract 0.3%, peptone 0.75%, yeast extract powder 0.5%.(4) The fermentation conditions of initial pH, broth content, shaking speed and cultivate method etc. were further explored on the basis of optimization results of medium by single factor and orthogonal test. The results showed that under the optimal culture conditions of YM medium, initial pH5.0, broth content 75 mL/500mL flask,20℃, shaking speed 180 r/min and batch culture in dark place, amount 1.36 mg/mL of mycelia biomass and 1.60 mg/mL extracellular polysaccharide yield could be obtained respectively after 12 days in submerged cultures.(5) For confirm the best extraction technology of polysaccharides of Fomes offcinalis Ames as the aim production of fermentation, we contrastively studied the extraction of hot water, dilute acid, dilute alkali and ultrasonic and ascertained finally that the best extraction process was ultrasonic-assisted hot water extraction. In order to obtaining the suitable extraction process, single factor and orthogonal test were carried out and recovery rate was the only target. The results showed that 66.5mg intracellular polysaccharide can be extracted every gram dry mycelia under the conditions of ultrasonic power 90W, ultrasonic time 15min, extract temperature 90℃, ratio between dry mycelia and water 1:20, extract time 3 hours and maximize extracellular polysaccharide recovery rate can be achieve 92.1% under the conditions of ethanol concentration 60%, precipitate temperature 4℃, solution pH7.0, precipitate time 24 hours.

Related Dissertations

  1. Development of the Platform for Compressor Optimization Design and Aerodynamic Optimization Design in the Transonic Compressor,TH45
  2. Investigation of Turbine S2 Stream Surface Direct Problem Aerodynamic Optimization Design,V235.11
  3. Research on Dam-Break Probability Analysis Method for Dangerous Reservoir,TV697
  4. Reseach on Optimal Control of Elevator Group Based upon Ant Colony Algorithm,TU857
  5. Research on High Efficiency Interior Permanent Magnet Synchronous Motor,TM341
  6. Application of Interior-Point Theory for Reactive Power Optimization in Large Power Systems,TM714.3
  7. The Basic Research of Axial Flux Inductor Type High Temperature Superconducting Motor,TM37
  8. Construction and improvement of basic education reform process evaluation platform,G521
  9. Implementation Study of evaluation method of the high school IT courses,G633.67
  10. The Optimization of AVS Video Decoder on the PC Platform and Improvement of Field Decoding,TN919.81
  11. Research on Stability of Hierarchical Sattellite Network,TN927.23
  12. Query Processing and Optimization in Massive Multi-Database Integration,TP311.13
  13. The Design and Implementation of Security Mechanism of Embedded Dependable Computer,TP309
  14. Research on Feature Extraction and Classification of Tongue Shape and Tooth-Marked Tongue in TCM Tongue Diagnosis,TP391.41
  15. Research on Evaluation of Simulation Systems Based on Fidelity,TP391.9
  16. Research on Predictive Control and Simulation of pH Value Based on the Confection Process of Sodium Nitrate,TP273
  17. Process Support Vector Machine and Its Application to Satellite Thermal Equilibrium Temperature Prediction,TP183
  18. Haihong Fruit Vinegar Processing Technology,TS264.22
  19. Penicillium Expansum TS414 Lipase in Pichia Pastori: Expression, Purification and Enantioselective Esterification of (R, S)-naproxen,Q814
  20. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  21. Study of Mutation and Breeding of Griseofulvin Produccing Strain FS80,TQ927

CLC: > Industrial Technology > Chemical Industry > Pharmaceutical chemical industry > The Chinese herbal medicine preparations of the production
© 2012 www.DissertationTopic.Net  Mobile