Dissertation > Excellent graduate degree dissertation topics show

Establishment of the Middle Cerebral Artery Occlusion Model and Study on Changes of NGB, SOD and MDA in Rat Following Cerebral Ischemic-Reperfusion Injury

Author: ZhangRongZuo
Tutor: WangHangYan
School: PLA Postgraduate Medical School
Course: Pediatrics
Keywords: Focal cerebral ischemia Animal models Sprague-Dawley rats Cerebral ischemia -reperfusion injury Ischemic penumbra Neuroglobin Superoxide dismutase Malondialdehyde
CLC: R-332
Type: Master's thesis
Year: 2007
Downloads: 337
Quote: 0
Read: Download Dissertation


The first part : the middle cerebral artery ischemia reperfusion model purpose : to establish a simple, reliable , and less invasive Sprague-Dawley rats with focal cerebral ischemia model . Method: the suture method to reference Zealonga and Koizum , to improve production of Sprague-Dawley rats with focal cerebral ischemia model , and subsequently underwent neurobehavioral examination , TTC staining judge infarction results in order to observe the model reliability . Results: The model rats neurological dysfunction , brain tissue ischemia and infarction . Conclusion: The modified model prepared simple , reliable results , trauma , good stability, is the ideal experimental animal model used to study focal cerebral ischemia . Part II: cerebral ischemia reperfusion injury NGB SOD , MDA changes Objective: To study the rat cerebral ischemia-reperfusion injury cerebral myoglobin (neuroglobin, NGB) and serum superoxide dismutase ( superoxide dismutase , SOD expression changes ) , malondialdehyde (malondialdehyde, MDA) and reperfusion time relationship . Methods: The rat cerebral ischemia-reperfusion injury in animal models , different times of the cerebral cortex in the determination of injury ischemic penumbra the NGB change and serum SOD activity and MDA content changes , compared with sham-operated group . Results: Compared with the sham group at each time point , the cerebral cortex ischemic penumbra NGB positive neurons increased and then decreased the trend ; SOD activity first and then decreased , MDA content increased at first and then decreased , and two the existence of a negative correlation . Conclusion: cerebral ischemia and reperfusion injury NGB , SOD , MDA, changes in a certain time regularity , prompted NGB adaptive protective effect of ischemic brain injury , SOD and MDA serological level changes can be a better representative repair the damage situation of the nerve cells in the brain , may be a new quantitative indicators , play a role in the hypoxic-ischemic brain injury disease early discovery and treatment evaluation .

Related Dissertations

  1. Experimental Study of Neural Stem Cells around the Lateral Ventricular Zone of the Hydrocephalus Rats,R742.7
  2. Study on Extraction and Properties of Superoxide Dismutase from Goose Blood,TS254.9
  3. The Expression Mechanism of Calmodulin in Rat with Brain Contusion,R651.1
  4. The Relevance Research of Neuroglobin and Caspase-3 in the Hippocampus CA1 Regin in Global Cerebral Ischemia-reperfused Rats,R741
  5. Neuroprotective Mechanism of NBP Preconditioning in Cerebral Ischemia Reperfusion Injury on Sprague-Dawley Rats,R743.33
  6. Establishment of Subcutaneous Transplanted Tumor Model of Skin Adenocarcinoma in Balb/c Mice,R739.5
  7. Exogenous PCr anti- animal brain ischemia-reperfusion injury and brain-derived experimental study MODS,R965
  8. Shamisen Medicinal and Edible Herbs on glucose and lipid metabolism in the metabolic syndrome and antioxidant effects,R285.5
  9. Berberine on serum levels of superoxide dismutase and malondialdehyde levels in,R743.33
  10. Perfusion CT original image identification infarction and ischemic penumbra center,R743.3
  11. Studying on Environmrntal Biomarkers Induced by PAEs,X171.5
  12. Mechanisms of EGCG Attenuating Focal Cerebral Ischemia and Reperfusion Injury in Rats,R285.5
  13. Cytokines in C57BL/6 Mice Model of Hepatitis B Virus Infection,R512.62
  14. Transplantation of Umbilical Cord Mesenchymal Stem Cells into Cisterna Magna for Cerebral Hypoxic-ischemic Rat Model,R722.1
  15. The Microcirculation Dysfunction and the Expression of Fgl2 in the Rat Model of Acute Cerebral Ischemia/reperfusion,R743
  16. Effects of Rem Sleep Deprivation on CRF and CRF Receptors mRNA in Depressive Rats’ Medulla Oblongata、hippocampus and Hypothalamus Treated with Chronic Stress,R749.4
  17. Experimental Study of Th17 Related Factors of Animal Models Infected with Mycoplasma Suis,S858.28
  18. Effects of Lidocaine on Liver Injury Induced by Intestinal Ischemia-Reperfusion in Young Rats,R965
  19. Experiment Study of Low-level Semiconductor Laser Therapy to Oral Ulcer,R781.5
  20. Diffusion Tensor Imaging Using 3.0T MR Scanner in Rat C6 Glioma Model and the Correlative Study of FA Value in Infiltrated Peritumoral Edema Tissue and AQP1,R739.4
  21. A Study of Schizophrenia Model by Chronic and Acute NMDAR Antagonist MK-801 Exposure in Perinatal and Adult Rats,R749.3

CLC: > Medicine, health > Medical research methods > Experimental medicine, medical experiments > Medical Laboratory Animal Science
© 2012 www.DissertationTopic.Net  Mobile