Dissertation > Excellent graduate degree dissertation topics show

A Study on the Microbial Characteristics of a Fluidized-pellet-bed Biareactor

Author: WangLi
Tutor: WangXiaoChang
School: Xi'an University of Architecture and Technology
Course: Municipal Engineering
Keywords: Biological granulation fluidized bed reactor Microbial properties Cell culture PCR-DGGE Phylogenetic analysis
CLC: TU992.3
Type: Master's thesis
Year: 2007
Downloads: 115
Quote: 1
Read: Download Dissertation

Abstract


Order to study the characteristics of the microorganism in the biological granulated fluidized bed sewage treatment reactor , respectively, from a biological granulated fluidized bed 10cm, 60cm, 110cm sampled at (water and particles of sludge), and the use of traditional cell culture method , detection of the bacteria The total number of denitrifying bacteria and anti - sulfide bacteria . Meanwhile, the application of molecular biology to study microbial characteristics in the bio- granulation fluidized bed . Granular Sludge bacterial genomic DNA directly extracted by cell lysis to the true bacterial and archaeal 16S rRNA gene universal primers 530F/1490R PCR amplification of genomic DNA of the the extracted bacteria in granular sludge , about 1kb PCR amplification products purified and separated by denaturing gradient gel electrophoresis (DGGE) for microbial community DNA fingerprint analysis , the characteristic bands for recycling sequencing and DGGE separation of the phylogenetic tree . Bacterial culture results show , the entire cross-section of the bio - granulation fluidized bed reactor , especially in the bottom of the reactor , there is a very rich aerobic microorganisms . Reactor also exist , such as denitrifying bacteria , anti- bacterial sulfide facultative anaerobic and obligate anaerobic microorganisms . PCR-DGGE results showed that the microbial community in granular sludge biological granulation fluidized bed reactor is very rich , in at 10cm microbial species reach 23 , 60cm at 21 and 110cm at 20 ; biological granulation fluidized bed at different heights has some endemic genera and Total species reactor at different heights microbial community succession is not obvious, the microbial community similarity of 83.1% , a more stable community structure . Phylogenetic analysis of bio- granulation fluidized bed microbial majority belong to the bacterial domain covers 18 taxa of bacteria domain , of which 61% ( 11 / 18 ) belong to the Proteobacteria , 17% ( 3 / 18 ) belong to the actinomycetes , 11% ( 2 / 18 ) of the low- GC Gram- positive bacteria , 11% ( 2 / 18 ) belong to other unknown bacteria .

Related Dissertations

  1. Comparison of Genetic Diversity of Total Planktonic Bacteria and Active Planktonic Bacteria in Huguangyan Maar Lake in Summer,Q938
  2. Study on Phylogenetic Diversity and Bioactivity of Symbiotic Fungi of Coral,R284
  3. Establishment of the Molecular Identifying System of Curvualria and Application in Difficult Species,Q949.32
  4. Soil Eoclogical Risk Evaluation of Transgenic Bt Rice Through Nematode Community Analysis,S154.1
  5. Studies on Microbial Contamination and Microbial Decontamination of Chemical Substances of Fresh Chicken,TS251.1
  6. Effect of Organic Nutritiveadditives on Soil Micro-ecological Restoration and Its Mechanism.,S143
  7. Ecological Control of Tobacco Bacterial Wilt in Continous-cropping Field and the Microecological Mechanisms,S435.72
  8. Identification and Lipopeptide Analysis of Biocontrol Bacillus Spp. Isolated from Tibet,S476.1
  9. Fine Mapping of Inter-Subspecies Hybrid Pollen Sterility Gene in Rice (Oryza Sativa L.) and Phylogenetic Analysis,S511
  10. Investigation on the Diversity and Phylogenetic of Methanogens and the Relationship between Methanogenesis and Acetogenesis in the Hindgut of Pigs,S828
  11. Effect of Probiotics on Growth Performance, Digestive Enzymes Activities, Immunity and Gut Microbiota of Epiniphelus Coioides,S917.4
  12. Identification of Biological Characteristics with Human Keloid Fibroblast-like Mesenchymal Stem Cells,R622
  13. In Vitro Culture and Identification of Vascular Smooth Muscle Cells from Rat Intra- and Extracranial Arteries,R743.3
  14. Isolation and Cultivation of Rabbit Adipose-derived Stem Cells and the Influence of Platelet-rich Plasma on the Cell Proliferation,R329
  15. Primary Research on Strring Culture and Storing of Human Dermal Fibroblasts,R329
  16. Phylogenetic Relationship and Biogeography of Thoreales Based on Gene Sequences,Q941
  17. A Simple and Efficient Method That Uses Low Concentration Fetal Bovine Serum to Culture and Purify Schwann Cells,R329
  18. Influence and Significance of MMP-2 and VEGF of Hemangioma Endothelial Cells in Vitro Culture by Propranolol,R739.5
  19. Fibroin microspheres and their use as cell carriers in study,R318.08
  20. Effect of Different Glucose Concentrations on Autophagy in Neonatal Rat Cardiomyocyte Culture,R542.2
  21. Effects of TLR2 on IL-8 and γ-IFN Secretion of Keratinocytes in Dermatophytic Infections,R756

CLC: > Industrial Technology > Building Science > Municipal Engineering > Drainage works ( ditches works,sewer works) > Sewage , sludge handling and use of
© 2012 www.DissertationTopic.Net  Mobile