Dissertation > Excellent graduate degree dissertation topics show

Study on Molecular Traceabilitry of Yak Meat Products

Author: HeJianWen
Tutor: HanJianLin；LuoYuZhu
School: Gansu Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Consumption of beef products Cytb gene Three specific SSR Source of the bovine species Molecular retrospective
CLC: S823.85
Type: Master's thesis
Year: 2010
Downloads: 46
Quote: 1
Read: Download Dissertation

Abstract


Level of mitochondrial and nuclear genes in order to establish the yak, cattle ( Bos indicus and Bos taurus ) and buffalo meat fast and accurate identification method , source of yak meat products traceability and identification of species composition , yak , cattle , buffalo meat as research material and the yak meat products derived from 10 different manufacturers , to try and systematically compare the guanidine isothiocyanate , phenol - chloroform extraction method and kit extraction DNA was extracted from the effects of a comparative analysis , and Cyt b gene and three specific microsatellite loci were detected , mainly to obtain the following results : 1 through quantitative analysis , gel electrophoresis and gradient PCR fragment size amplified by means of guanidine isothiocyanate , phenol - chloroform the quantity and quality of the genomic DNA extraction method of the extraction method and kit were compared. The study suggests that the guanidine isothiocyanate method is simple , fast , low-cost to high-quality genomic DNA extracted from yak meat products . Cyt b gene (472 bp) region yak, cattle and buffalo specific restriction sites : yak restriction sites GACGCNNNN / restriction fragment of 220 bp and 252 bp , respectively ; ordinary cattle restriction sites GGGCC / C, restriction fragment of 173 bp and 299 bp , respectively ; buffalo restriction sites for CCANNNN / NTGG restriction fragments of 69 bp and 403 bp , respectively , and no cross- digested phenomenon can be used in the identification of the sources of the mixed beef cattle . Cyt b gene fragment sequencing to detect 135 samples by 10 manufacturers , 30 bags of yak meat products found the 68 yak sequences , only 50.4% ; addition there is a sequence of 51 cattle and 16 buffalo sequence , accounted for 49.6% . Beef , buffalo meat doped into a very serious problem in the yak meat products . 4. Study found that the combination of three specific microsatellite loci detection results for the presence of genes penetration of livestock breeds , in order to get reliable conclusions .

Related Dissertations

  1. Studies on Molecular Traceability of the Species Origin of Sheep and Goat Meat Products,S826
  2. Molecular Phylogenetic Analysis of Polyrhachis (Hymenoptera: Formicidae: Myemicinae),Q969.2
  3. The Research of Six Kinds of Turtles on Molecular Classification,Q959
  4. Male and Femal Origin of Four Chinese Yellow Cattle,S823.81
  5. A Forensic Medicine Application Study of Necrophagous Blowflies Species Identification Using Insect Molecular Systematics,D919.4
  6. Molecular Systematic Study on Several Species of Camponotus (Hymenoptera: Formicidae),Q961
  7. Study of Genetic Diversity in Spanish Mackele (Scomberomorus Niphonius) and Anchovy (Engraulis Japanicus),Q953
  8. Characterization of the Sequence of Cyt b Gene & D-loop Region of Tiger mtDNA and Their Significance in Subspecies Identification,Q953
  9. The Yellow Sea and East China anchovy Genetic diversity of natural populations,Q953
  10. Polymorphysm Analysis of Mitochondrial D-loop Region, ND5 Gene and Cyt b Gene in Chinese Yellow Cattles,S823.81
  11. Molecular Evolution and Phylogeny of Some Species of China Aquatics Hemiptera Based on Cytb Gene,Q969
  12. Phylogeny of Partial Species of Coreidae Based on Mitochondrial Cytochrome b Gene Sequence,Q969
  13. Molecular Clock Test and Phylogenetic Status of Leiqiong Cattle and Haizi Water Buffalo Based on Analysis of Sequences of Cytochrome b Gene and Alcohol Dehydrogenase Gene,S823
  14. Evolution Analysis of Chinese Macaques and the Study of Special Genetic Marker,Q953
  15. Research on Genetic Identification and Anti-viral Effect of Edible Bird’s Nest,R285.5
  16. Morphological Study of Yak Placenta During Gestation,S823.85
  17. The Isolation and Indentification of Yak Placental Trophoblastic Cells,S823.85
  18. Effect of Fertilizer Treatment to Alpine Meadow on Antioxidant System Function in Yaks,S823.85
  19. Cloning and Modeling of Yak Hemoglobin,S823.85
  20. Yak Domestication and Adaptation: Evidence from Mitochondrial Genome,S823.85

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Livestock > Cow > Variety > Yak
© 2012 www.DissertationTopic.Net  Mobile