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Studies on Producing Feed Activity Small Peptide by Fermentation

Author: HeYongJin
Tutor: XieBiFeng
School: Fujian Normal University
Course: Fermentation Engineering
Keywords: Small peptides Soybean meal Liquid fermentation Raw material fermentation
CLC: S816
Type: Master's thesis
Year: 2010
Downloads: 115
Quote: 0
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Abstract


Small peptides with a variety of biological functions is a new type of green protein feed or additives. Forage production by microbial fermentation of small peptides , animal nutrition research has become a hot area . This article in the feed waste microbial strains isolated and screened excellent and excellent preservation laboratory strains of activation as the study species in soybean flour as fermentation substrate for fermentation, to obtain simple and economical process optimization studies . Made the following findings : a separation method using microorganisms ( diluted coating separation method and dilution pour separation ) and screening methods, and successfully isolated and screened to excellent microbial strains . Through microbial growth characteristics , colony characteristics , growth curve , reproduction and other aspects , the analysis showed that the microorganism was isolated and screened to Bacillus subtilis and Rhodotorula Y3. 2 study of environmental factors on isolated and screened to excellent growth of microbial strains . Results obtained from Bacillus subtilis and Rhodotorula Y3 best initial culture medium volume , pH , and temperature . 3 is soybean powder as raw material , fermentation of microorganisms excellent screening strains for fermentation . Microbial protein soybean meal in macromolecular degradation into small peptides . By biuret method for the determination of small peptide content as an indicator of the microbial degradation of soybean fermentation conditions were optimized . The results showed that: the optimum degradation conditions, degradation of Bacillus subtilis fermentation medium 02-03 peptide content of 58.86% , higher than before optimization 37.99% ; while Rhodotorula Y3 degradation fermented soybean powder content of small peptides from 20.87% up to 56.96% . 4 study screened a fermented soybean meal can better grow and degrade matrix proteins on higher ability Y3 strain Rhodotorula intended to select a simple operation , low energy consumption , less investment microbial fermentation raw material prepared by fermentation of small peptides the fermentation process parameters. Tests to determine soybean powder as fermentation substrate to Rhodotorula Y3 as starting strain , through raw material fermentation, fermentation billet small peptides content from about 4% to 27 % or so.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > General Animal Science > Feed
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