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Inspection and Analysis Ecological Characteristics of Rabies Virus Flury-lep Strain

Author: LuoShiMin
Tutor: ChenTieQiao；HuHui
School: Hunan Agricultural University
Course: Clinical Veterinary Medicine
Keywords: Rabies vaccine Ftury-lep strain Ecological characteristics
CLC: S855.3
Type: Master's thesis
Year: 2010
Downloads: 47
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Abstract

The prevention and control of rabies (Rabies) immunization-based rabies vaccine as a live attenuated vaccine, rabies attenuated live vaccine seed virus generally Flury-lep strain. Therefore it is necessary to conduct a comprehensive evaluation of the ecological characteristics of the strain of the rabies virus Flury-lep data accumulated to ensure and improve the quality of rabies vaccine in China. In this study, Flury-lep strain of the rabies virus cultured BHK-21 cells, embryos and mouse brain morphology were observed by scanning electron microscopy. Under the electron microscope, the tip of the virus particles was obtuse or sharp curved, while the bottom end of the viral particle is flush. The rabies virus particle shape of the bullet-shaped or cone. Flury-lep strain of the rabies virus in cultures of cells, embryos and mouse brain gross morphology consistent are bullet-shaped or cone, but there are some small differences in electron microscopy images show: BHK-21 cell cultures and chick Flury-lep strain of the rabies virus in culture shape of a bullet-shaped rabies virus Flury-lep strain of mouse brain training most of the shape of a cone, the number of virus particles in the shape of a bullet-shaped. BHK-21 cell culture and embryo culture rabies virus Flury-lep strain of the virus particle shape and size is more consistent, and the culture of mouse brain rabies virus Flury-lep strain of the virus particle shape and size differences. The apparent difference in the size of the viral particles in the different cultures. Comparative Analysis of virus strain of the rabies virus Flury-lep particle shape and size from the different cultures, the the Flury-lep rabies virus strains of the virus particle morphology is more stable from the morphology of the size and the same culture, wherein the viral particle morphology consistent point of view, the application of rabies virus the Flury-lep strains to prepare rabies vaccine is the best with the proliferation of BHK-21 cells cultured the Flury-lep strains of rabies virus. This study examined the BHK-21 cells and chick embryo culture Flury-lep strain of the rabies virus hemagglutination of goose erythrocytes. BHK-21 cells and chick embryo proliferation rabies virus Flury-lep strain, at 4 ℃ HA test results show that: Flury of-lep poison in the 4 ℃ BHK-21 cell culture was able to agglutinate goose erythrocytes HA titers of neat and high titer, the four samples HA titer 1:256,1:256,1:256,1:256; the chick cultures HA titer Range neat low titer HA titer of the four samples was 1:16,1:8,1:8,1:8. From the results, the amount of virus in BHK-21 cells cultured matter high high titer hemagglutination comparison, both the efficiency of virus proliferation BHK-21 cells and chick embryo proliferation rabies virus Flury of-lep strain is there are significant differences in the proliferation of cultured virus glycoprotein activity the Flury-lep strain of the rabies virus is better than with the chick embryo proliferation Flury-lep strain of the rabies virus, BHK-21 cells proliferation. Therefore, the preparation of rabies virus the Flury-lep strains of attenuated vaccine should be chosen BHK-21 cells proliferation the Flury-lep strains of rabies virus. In this study, Flury-lep rabies virus glycoprotein gene and the cloning and sequencing of the amino acid sequence comparison with other rabies virus strains glycoprotein gene and amino acid sequence homology. According to the sequencing of the rabies virus of Flury-lep strains glycoprotein gene nucleotide sequence, the DNAstar software deduced the amino acid sequence of the Flury-lep strain of rabies virus glycoprotein. Comparison of the rabies virus glycoprotein the the Flury-lep glycoprotein gene and 10 common rabies virus strains glycoprotein gene nucleotide sequence and deduced amino acid sequence homology to compare the results of nucleotide sequence homology of 90.2% -97.5% 90.4% -95.4% amino acid homology.

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