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Liriope muscari (Decne.) Bailey, belonging to the Lily Family, is an important traditional Chinese herbal medicine. It’s distributed manily in Fujian Province, which grows in Quanzhou and Putian. Its tubers are employed in traditional Chinese medicine with noursing yin and engendering fluid, moistening lung for arresting cough, clearing heart and clearing dysphoria. It manily cures of dry cough with thick phlegm, virtual fatigue cough, upset insomnia, quench thirst with internal heat and intestinal constipation dry. Recently, reports as to aspects of molecular study on L.muscari is very few, especially the research on genetic diversity at level of DNA, which restrains the development and utilization of L.muscari. The paper studies the genetic diversity, based on 47 material of L.muscari cultivars, using RAPD、ISSR、SRAP and TRAP-four kinds of molecular markers , and evaluates the genetic diversity of these plants with morphological markers. The main results are as follow:1. Base on 12 major quantitative characters of L.muscari, variation, correlation and factor analyses were used to statistically research the properties of the plant. Considerable differences on the characters were surveyed among the individual L.muscari. The aboveground and underground characters also had significant correlations, so the underground growth conditions were judged from the aboveground growth conditions. It provides a theoretical foundation for the choice of breed. The relationships among the characterics are complex. So the relation between characteristic selection and new variety breeding should be necessary noticed.2. The method of improved CTAB was used to extract the genomic DNA of species in L.muscari. The RAPD, ISSR, SRAP and TRAP analysis system were set up and studied the genetic diversity level of the 47 L.muscari accessions.1) Five RAPD random primers, selected from nine primers, were used to amplify the L.muscari and fourty-nine DNA bands were amplified, of which the percentage of polymorphic loci was 65.31%. Shannon’s information index, Nei’s gene diversity, effective number of alleles and observed numbers of alleles were respectively 0.2070, 0.1292, 1.2105, 1.6531. Cluster analysis using UPGMA method showed that genetic similarity cofficient ranged in 0.4899-0.9999. As a group, the wild species from Putian had the more genetic difference compared the landrace.2) Eighteen ISSR primers, selected from thirty-one primers, were used to amplify the L.muscari and one hundred and thirty-one DNA bands were amplified, of which the percentage of polymorphic loci was 63.4%. Shannon’s information index, Nei’s gene diversity, effective number of alleles and observed numbers of alleles were respectively 0.2178, 0.1363, 1.2166, 1.5968. Cluster analysis using UPGMA method showed that genetic similarity coefficient ranged in 0.6755-0.9839. As a group, the wild species from Putian had the greatest genetic difference compared the landrace.3) Fifteen SRAP primer paris, selected from ninety primer pairs, were used to amplify the L.muscari and three hundred and twenty-three DNA bands were amplified, of which the percentage of polymorphic loci was 88.47%. Shannon’s information index, Nei’s gene diversity, effective number of alleles and observed numbers of alleles were respectively 0.3190, 0.1977, 1.3097 and 1.896. Cluster analysis using UPGMA method showed that genetic similarity coefficient ranged in 0.6634-0.9156. As a group, the wild species from Putian had the greatest genetic difference compared the landrace.4) Fifteen TRAP primers pairs, selected from one hundred and sixty primer pairs, were used to amplify the L.muscari and three hundred and thirty-five DNA bands were amplified, of which the percentage of polymorphic loci was 96.42%. Shannon’s information index, Nei’s gene diversity, effective number of alleles and observed number of alleles were respectively 0.3340, 0.209, 1.332 and 1.964. Cluster analysis using UPGMA method showed that genetic similarity coefficient ranged in 5244-0.9761.3. Using the phenol-sulfuric acid method detected the content of polysaccharides in Radix L.muscari(Dence.) Bailey. The maximum absorption wavelength was 490nm. Linear range of glucose was 7.433-51.80mg/L, r=0.9995. The average recovery was 102.29%, RSD=1.34(n=6). The conversion factor (f) was 1.22. The polysaccharide content in Radix was computed by the formula(C×D× f)/W×100%. The average content of polysaccharides in L.muscari was 10.25%, which the maximum content was sample No.28, 16.48%, and the minimum content is sample No.14, 4.08%.
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