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Pears pear belong to the Rosaceae subfamily, many varieties, for a long time there are a lot of problems in the classification. The purpose of this paper is to study the major pear varieties cytoplasmic genetic polymorphism. Using PCR-RFLP method, on the extracted total DNA using 10 chloroplast General cited material amplified PCR products were digested with restriction cutting enzyme Alu Ⅰ with Hae Ⅲ in Hinf Ⅰ Hin6 Ⅰ, Rsa Ⅰ, Mva Ⅰ and TaqI carried digestion, on 19 kinds of pears (including Xinjiang pears system, Pear system, pears system, autumn pears system, Pyrus, pear system) chloroplast genome trnS-trnfM non-coding region were cloned, sequenced. Application DPS v7.05 DNAMAN, DNAStar, ClustalX-1.83, PHYLIP-3.68 software for analysis. Sequence alignment, clustering analysis, and finally determined according to the results of the genetic relationship of the measured molecular sequence, a phylogenetic tree. The results showed that: only seven pairs (10 pairs of primers cp01 cp 02, cp 03, cp 04, cp, 06, cp 09, cp.) In Pyrus amplified a specific band, Pyrus plant chloroplast genome sequence is very conservative, three primer pairs (cp05, cp07, cp08) not Pyrus amplified bands. 931 primer / digestion portfolio, cp09/Mva Ⅰ, cp03/Hin6 I restriction sites were significantly different. Of Pyrus plants the cpDNA trnS-trnfM area cloning, sequencing, the resulting sequence length: the longest sequence of Korla Pear and pear (1642bp), apple-pear, the pear, Tsz pear, ivory, emerald volts sequence shortest (1272bp). DNAMAN software to compare and analyze the sequence: the Korla Pear Pear homology is: 85.01%, Xinjiang pears homology: 78.60% with pears homology: 78.28 %, with homology of pear system: 77.47%, Pyrus ussuriensis system homology: 77.91%. ClustalX software completely than on the results of the cpDNA trnS-trnfM region sequence variable sites using the neighbor-joining method of PHYLIP-3.68 software phylogenetic trees. The black acid pears and Beijing white poly class, volt eggplant and body I do not know clustered into one group, the Winter bar and the new century clustered into one group. The the Korla Pear and Xinjiang sentence pear, Jinchuan Sydney, Beijing white, black acid pears poly class. The main conclusion of the experiment: Korla Pear and apple-pear, pear, Zaosu Tsz pears, Dangshan, the Jinchuan Sydney, Xinjiang sentence pear, the Jinfeng average distance coefficient small, compared with the average distance of the other pear coefficient large. The Zaosu apple pear and body I do not know hybrid varieties. Zaosu apple-pear homology: 99.45% homology with the body I do not know: 97.76%. Jinfeng is apple pear and Tsz pear hybrid varieties. Jinfeng apple-pear homology: 78.76% the the Jinfeng and Tsz pear homology: 78.71%. Compared to offspring, and maternal and paternal homology. Korla Pear sequence of more than 18 kinds of pears, and Dangshan highest homology: 98.36%. Korla Pear bretschneideri system and Xinjiang pears kinship closer with pear system and Akiko pear kinship far. Therefore, the significance of this experiment is the combination of molecular biology and bioinformatics cytoplasm analysis of 19 pear varieties, find out bretschneideri system, Xinjiang pears system, pears, Pyrus, pear and autumn pears system plasmon The genetic diversity. To provide further genetic evidence for the study of the pear genus kinship.
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