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Tissue Culture Study of Three Carya Plants

Author: JiXiaoZuo
Tutor: LvFangDe
School: Central South University of Forestry Science and Technology
Course: Forest cultivation
Keywords: Hickory Tissue culture Blade Stem segments Browning prevention
CLC: S664.1
Type: Master's thesis
Year: 2009
Downloads: 116
Quote: 1
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Abstract

This selection of United States Hickory, Hickory, Zhejiang, Hunan pecan seedling and adult tree leaves and with a bud stem segments as test material. The screening of the best medium, explant selection, the stem culture and blade culture and browning and other issues to do a more comprehensive and systematic experimental study. The main results are as follows: 1. Pecan tissue culture leaves were cultured as test materials, should choose young leaves; Leaf Culture medium was DKW; Leaf Culture the lowest browning hormone combination of 2,4-D 1.0mg / L IB A2.0mg / L 6-BA0.02mg / L. 2 Pecan in tissue culture the pretreatment test materials control browning test, the use of 2% Na2S2O3 solution immersion test materials 20-30min conventional sterilization, and then tested material soaked in 0.2% Na2S2O3 solution sterilized by high pressure inoculated directly remove the control browning effect; concentration of 300mg / L PVP pretreatment test material 5min lowest browning rate of the test materials. Pecan Leaf Culture inhibit browning reaction test, The inoculated medium was placed in a 4 ° C in the refrigerator refrigerated 7d, and then got to continue to foster the culture chamber, prevention of browning the best; in the medium Add 5.0mg/LNa2S2O3 or concentration more than 60 mg / L PVP, the lowest browning rate of the test material. (4) in the prevention and control of pollution tests, pre-treatment with penicillin pecan leaves and added to the medium 4g / L carbendazim, can effectively control pollution rate of the test material. 5 the stem culture in Zhejiang pecan, best bud growth and differentiation and callus induction medium and hormone combination the DKW IBA2.0mg / of L KT8.Omg / L; the blade culture medium and hormone combination WPM NAA 2.0mg / L 6-BA1.0mg / L. The Hunan pecan stem segments bud growth and differentiation medium and hormone combination: the DKW IBA0.5mg / L KT2.0mg / L; callus induction best combination of hormones locations: IBA2.0mg / L KT8.0mg / L; Leaf Culture Media and Hormone combination: WPM NAA 1.5mg / L 6-BA3.0mg / L. Two pecan tissue culture medium supplemented with the optimal sucrose concentration of 30 g / L. Shoot tip culture browning control test: Zhejiang hickory and pecan, Hunan, 5.0mg/LNa2S2O3 added to the medium can effectively inhibit the shoot tip browning. Pecans in Zhejiang and Hunan pecan leaves tissue culture browning prevention the best antioxidants added to the medium and its concentration: Na2S2O32.0mg / L. When PVP pretreatment control browning test, the concentration of more than 200mg / L PVP soaking Zhejiang pecan leaf tissue culture inhibit browning reflect effect best; concentration of more than 300mg / L PVP soaking Hunan hickory leaves inhibit browning effect best. Subculture, for the medium of pecan pecan Zhejiang and Hunan DKW; the best subculture hormone combination, Zhejiang pecan IBA1.0mg / L 6-B AO.2mg / L, Hunan Hickory IBA2.0mg / L; two hickory Following culture medium pH of 5.8.

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