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In-Situ Detection of Carotenoid Biosynthetic Gene Expression during Cara Cara Navel Orange (Citrus Sinensis Osbeck) Fruit Development

Author: WenQiGen
Tutor: XuJuan
School: Huazhong Agricultural University
Course: Pomology
Keywords: Citrus Red meat Carotenoids Gene Expression RNA in situ hybridization
CLC: S666.4
Type: Master's thesis
Year: 2009
Downloads: 55
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Abstract


Citrus fruit rich in carotenoids , and in most cases the peel content than pulp . Object to the citrus red meat mutant in recent years has caused more and more attention , the one hand , these mutants to confer upon itself the superior appearance , on the other hand , for the class in the mechanism as well as citrus pulp of citrus fruit bud mutation occurred the carotenoid biosynthesis ideal material . In this study, the Cara Cara Orange (C.sinensis Osbeckcv 'Cara Cara') and Seike navel orange (C.sinensis Osbeck cv 'Seika') as the experimental material , the use of RNA in situ hybridization analysis of different fruit tissues carotenoid biosynthesis related gene expression . The main findings are as follows : 1 . Juice sac hand sectioning and microscopic observation tablet , Cara Cara Orange juice sac detected the presence of red colored linear body . Found a similar situation in a tomato and red grapefruit , with the fruit development of this shape colored body accumulated Cara Cara Orange juice sac . The different developmental stages of the Cara Cara Orange and Seike navel orange fruit samples made ??of paraffin sections using specific cRNA probes for in situ hybridization results : psy , pds , lcyb cara cara and Seike navel orange yellow cortex expression ; mRNA accumulate more close to the surface of the cortex cells could not be detected in the oil gland and its surrounding tissue mRNA accumulation ; different fruit development period mRNA accumulation differences ; white cortex and juice sac corresponding mRNA distribution no obvious yellow cortical tissue , but white cortex and juice sac psy, pds, lcyb expression can be seen below the yellow cortical tissue expression .

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CLC: > Agricultural Sciences > Gardening > Fruit trees gardening > Citrus > Orange
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