Dissertation > Excellent graduate degree dissertation topics show

The Construction of cDNA Libraries and Analysis of Differentially Expressed Genes at Maturation Stages of Ricinus Communis Embryo

Author: GaoYanPing
Tutor: ChenChunLiï¼›WeiWenHui
School: Huazhong Agricultural University
Course: Cell Biology
Keywords: Castor oil plant Suppression subtractive hybridization cDNA library RT-PCR
CLC: S565.6
Type: Master's thesis
Year: 2009
Downloads: 71
Quote: 0
Read: Download Dissertation

Abstract


Castor (Ricinus communis L.) is important in today's world of special oil crops are widely distributed around the world . Castor fatty acid metabolism mechanism Few studies reported fewer fatty acid synthesis metabolic networks in particular, from the perspective of the castor embryo development research . How to find the key regulatory genes from the complex biochemical system , clarify its biological function and to transform the direction of human needs , on the the modified castor fatty acid composition to improve its fat content is important . Our country is the origin of the castor oil plant , oil grate the 5th castor varieties are endemic to China , this subject to castor oil grate \embryos at different developmental stages of gene expression differences in favor embryo development and the key to the regulation of lipid metabolism genes screened , and lay a foundation for later study . The experimental results are as follows : using suppression subtractive hybridization , \700 clones were randomly picked broth PCR detection screening of 596 positive clones , effective recombination rate in more than 85% , the average length of the inserted fragment of the library of about 500 bp . After dot blot screening selected 521 positive clones were sequenced , after the merge homology than 96 differentially expressed genes , including 31 unknown genes and 65 known genes , genes involved in fat synthesis , sugar decomposition , protein storage and degradation of other aspects . Detection of ricin different tissue expression through which protein castor oil , castor storage protein , Arabidopsis hydrolyzing a nitrile protein 5 gene and found that high expression levels in the embryo . To detect these gene expression in embryos of different developmental stages , and their temporal expression analysis using the same method . The results showed that the expression of several genes in the embryo , but the expression in embryos of different developmental stages .

Related Dissertations

  1. Analysis of Infrared Spectral Features and Identification of Al-responsive Genes in Small Black Soybean with Aluminum Stress,S529
  2. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  3. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  4. Isolation, Identification and Molecular Characteristics of Japane Encephalitis Virus in Henan Province,S852.65
  5. Studies on the Difference of PRRSV Infectious and PRRSV ADE,S858.28
  6. Construction and Ests Analysis of the Subtraction Cdna Library for Larvae of Lutjanus Sanguineus Induced by Vibrio Alginolyticus,S943
  7. Molecular Cloning, Prokaryotic Expression and Characterization of a Novel Trypsin Inhibitor in the Frog, Lepidobatrachus Laevis,Q78
  8. cDNA-cloning and Expression analysis of Phosphate Stress Induced Purple Acid Phosphatase Genes and transcription Factor Genes from Seeding Wheat,S512.1
  9. Construction and Primary Analysis of Differentially Expressed cDNA Library of Alfalfa by Using Suppression Subtractive Hybridization under the High Temperature,S541.9
  10. Study on Key Technology and Its Physiological Basis of Virus Elimination and Rapid Propagation of Phalaenopsis,S682.31
  11. Identifies Differential Gene Expression Related to Gravitropism-Horizontal Gravitropism in Creeping Shoots of Ground-Cover Chrysanthemum,S682.11
  12. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  13. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  14. The Detection and Significance of CK 19 mRNA and MUC1 mRNA in Peripheral Blood in Cervical Cancer Patients.,R737.33
  15. Effect of HCO3-, K+ and HSO3- on rbcL and rbcS Gene Expression and Photosynthesis of Soybean Seedlings,S565.1
  16. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  17. Primary Study on CLC1 Gene Identification and Its Function in Glycine Max, Salt-born Glycine Soja and Their Hybrid,S565.1
  18. Cloning of Celluase Gene cel6B from Lentinula Endodes and Its Expression in Escherichia Coil,S646.12
  19. The Study of Fluorescence Quantitative PCR for Detection of Streptococcus Pneumoniae in Sputum of Acute Exacerbations of Chronic Obstructive Pulmonary Disease,R563.9
  20. The Regulation and Significance of NHE1 in Acidic Micro-environment of Gastric Carcinoma,R735.2
  21. A Preliminary Study on Differentiation of Adipose-derived Stem Cell to Odontoblast-like Cell,R78

CLC: > Agricultural Sciences > Crop > Economic crops > Oil crops > Castor
© 2012 www.DissertationTopic.Net  Mobile