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Studies on the Ferment Technics of Trichoderma Harzianum and Effect of Fermentation Broth on the Growth of Vegetables

Author: YouChengBing
Tutor: HuaRiMao
School: Anhui Agricultural University
Course: Pesticides
Keywords: Trichoderma harzianum Fermentation process Fermentation product Vegetables Research
CLC: S63
Type: Master's thesis
Year: 2006
Downloads: 166
Quote: 2
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Abstract

Based shaker fermentation fermentor H-13 strain process were studied. Made a preliminary attempt to promote the development of Chinese cabbage growth preparations, by cabbage biological determination, filter out the best emulsifier Add concentration. The fermentor liquid to regulate the growth of vegetables tested, compare the different fermentation broth deal with the growth targets of the test vegetables each initial clarify the role of the characteristics of the fermentation broth, and filter out the best fermentation of the growth-promoting effect of the test vegetables concentration, application and frequency of application. Provide a theoretical basis and practical experience for the industrial production of the fermentation broth and practical application. 1 H-13 fermentation tank fermentation filtrate by weighing up to 30.8%, with the shaker fermentation broth weight gain difference was not significant. Different fermentation times and different extraction methods from the fermentation broth cabbage fresh weight. Fermentation 48h optimum fermentation end time, the filtrate is an optimum extraction of the fermentation broth. Add 5% B 2 in the fermentation broth can significantly enhance the growth-promoting effect of the active substance, Chinese cabbage fresh weight compared to water control weight gain of 41.8%, representing a treatment weight gain of 11% was not added, also higher than the rest of the emulsifier processing, and the difference significant. The proved Add 5% B is the best choice. The preparation made heat storage after 14 days, the effect of weight gain less heat storage decline was not significant the fermentation broth difference additives. 100X processing cabbage weighing up to 37.0%, as compared to the other concentrations significantly different treatment, promote the growth of Chinese cabbage for the fermentor liquid concentration. H-13 fermentation liquid to improve the tomato seed germination energy, germination rate, germination index and seedling dry weight of no significant role in promoting significant difference from the control with water. But can increase fermentation broth processing tomato seedling root length, shoot length, seedling fresh weight and vigor index. 25X processing, seed vigor index of water was increased by 55.3%, a significant difference is the concentration of the best tomato seeds. The 100X IRRIGATING deal with the tomato plant height, stem diameter, number of leaves, seedling fresh weight and seedling dry weight of five indicators of measurement results are better than water control. Stem diameter and shoot fresh weight a 25X IRRIGATING processing results are not significant differences. In actual applications, 100X IRRIGATING the treatment best seedling processing. 200X foliar spray processing plant height, early flowering time, flowering tomato growth traits and fruit diameter, fruit weight and fruit per plant fresh weight yield traits are better than water control. Plant total fresh fruit weighing 435.89g, more water treatment increased 256.4%. Quality measurement: the process for increasing fruit Vc, soluble protein content and sugar-acid ratio has a significant role in promoting. And make the tomato plants against effective against viral diseases. Field experiments also proved 200X foliar spray twice fermentation broth to promote the growth of tomato processing. 5 50X 2 times IRRIGATING treatment significantly stimulated the growth rate of the pepper fruit. Two harvest fruit fresh weight 321.56g is about 12 times the water control, all deal with the strongest growth-promoting effects. And the process in terms of height, stem diameter, early flowering, fruit diameter, fruit length, fruit weight single superior water control. Therefore, 50X 2 IRRIGATING treatment is best promote growth and processing of pepper. 6 after application to 14 days, the treatment of low concentration (400X) with the largest pro-celery stalk elongation effect. 7d and 14d elongation and water control significant difference. Spraying 14 ~ 28 days, moderate concentrations (100X) treatment showed the greatest effect in promoting significant differences in elongation and water control, there are also significant differences with the rest of the concentration process. It can be seen that the the fermentation broth 100X processing better holding effect on the growth of celery, the best pro-celery seedlings growth concentration. 7 100X foliar spraying combination IRRIGATING processing can increase the number of leaves of Basella, but also to promote the process and 25X IRRIGATING, processing the Basella up growth, and water control significant difference. Processing of fermentation broth leaves expand growth inhibition, leaf area is small compared to the water control. From the point of view of the fresh weight, 50X foliar application process leaves fresh weight and total fresh weight slightly higher than the water control, but increase production of Basella no significant role in promoting, total fresh weight increased by only 1.9%. H-13 is not suitable for fermentation broth unnecessary application of Basella.

Studies on the Ferment Technics of Trichoderma Harzianum and Effect of Fermentation Broth on the Growth of Vegetables

Abstract

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