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In this study, the response surface method for optimization on the submerged culture positive Russula (Russula vinosa) mycelium producing extracellular lectin culture conditions to determine the optimal conditions for production lectin : 3.04% starch , yeast extract 0.3 %, KH2PO40.2%, MgSO40.1%, liquid volume in 73 mL of a speed of 250 r / min , initial pH 5, fermentation time 5 days . Under this condition , the total activity of the lectin in the mycelium production intracellular up 72127.3 25044.2 increased compared with the total activity of the lectin in the initial culture conditions , a 1.88 - fold . The positive red Mycelial PB extraction , ammonium sulfate precipitation , DEAE-Sepharose FF ion exchange chromatography and Sephadex G-100 gel filtration purified a lectin (Russula vinosa Lectin RVL ) . RVL specific activity than crude extracts increased 73.3 times , activity recovery rate of 54.4% . By SephadexG-100 measured the RVL relative molecular mass of about 55kD. RVL on SDS-PAGE showed a single protein band , containing only one subunit . RVL physicochemical properties and biological activity . The results show that the RVL is a glycoprotein , wherein the saccharide content is 3.87% ; to detect agglutination activity of rabbit erythrocytes , the agglutination activity is stable within the temperature range of 20-60 ℃ ; pH in the range of 5-9 , RVL agglutination activity remained relatively stable ; RVL Mn2, Zn2, Ca2 different extent dependent effect ; for sugar specificity studies show that the D - mannose is a the RVL specificity inhibiting sugar , sucrose , D - galactose , N - acetyl-D- glucosamine in a certain extent on the agglutination activity inhibition ; RVL the amino acid composition analysis shows RVL contains 15 amino acids , wherein the highest content of histidine , not containing arginine and proline . Antibacterial experiments RVL tested bacteria are not inhibited mycelial growth of some fungi tested (red bread mold , Trichoderma viride , rice blast fungus , Aspergillus niger ) certain extent ; Aspergillus niger Trichoderma viride , Aspergillus flavus and rice blast fungus spore germination and no significant inhibition .
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