Dissertation > Excellent graduate degree dissertation topics show

Study of Antitumor Mechanisms of Compound Agent of Cordyceps Sinensis and Cordycepin in Hepatocellular Cancer HepG-2 Cell Line

Author: DingXiangPing
Tutor: MaLi
School: Lanzhou University
Course: Internal Medicine
Keywords: Compound Cordyceps preparations Cordycepin HepG-2 cells Telomerase survivin NF-κBp65 bcl-2
CLC: R735.7
Type: Master's thesis
Year: 2007
Downloads: 286
Quote: 0
Read: Download Dissertation

Abstract


The purpose of research Compound Cordyceps preparations - days Lectra (TLK) and its single-component cordycepin on human hepatoma cell HepG-2 anti-tumor effect of the molecular mechanisms and their impact on telomerase activity to explore Compound Cordyceps the formulations days Lectra single component The similarities and differences of the mechanism of action of cordycepin. Methods MTT assay the inhibitory effect of the drugs on HepG-2 cells; hepatoma cell morphological changes were observed under phase contrast microscope; ultrastructural changes observed by transmission electron microscopy; Hochest33258 staining and Giemsa staining apoptosis morphology changes; application flow cytometry (FCM) PI staining to detect cell cycle, apoptosis rate and bcl-2 changes, AV / PI double staining detects early apoptosis and necrosis; immunohistochemical methods Determination of the Survivin protein and NF-κBp65 of expression; using TRAP-PCR-silver staining method to detect telomerase activity of liver cancer cells. MTT assay shows within 72 hours days Lectra cells significantly inhibited (P> 0.05), Cordyceps known as significantly inhibited in a dose-dependent manner (P <0.01). Giemsa staining and transmission electron microscopy (TEM), inverted phase contrast microscope found days Lectra role in cell 48 hours after the cytoplasm vacuoles change degradable substances within the cytoplasm; cordycepin for 24 hours after cell rounding, floating chromatin nuclear fragmentation; Hoechst33258 of fluorescent staining shows days Lectra nucleus slightly concentrated cordycepin shown dark blue or pieces shaped nucleus, and typical apoptotic bodies; Immunohistochemistry showed Survivin protein and NF-day Lectra κB was downregulated (P <0.0005), and cordycepin on the expression of Survivin protein had no effect; the FCM test results show days Lectra role, increased the proportion of S-phase cells, G1 \u0026 G2 / M phase cells were decreased (P <0.0005 ), and the expression of bcl-2 decreased; the cordycepin role hypodiploid apoptosis peak, so that the cell cycle arrest in S phase. After Annexin-V/PI staining detected by flow cytometry days Lectra group obviously phosphatidylserine (phosphatdylserine, PS) film valgus cordycepin after showing early apoptotic graphics; TRAP-PCR-silver staining shows two can make telomerase activity lowered (P <0.01). Conclusion: days Lectra human hepatoma cell autophagy, leading to the degradation of cytoplasmic vacuolar degeneration, cytoplasmic contents, mild chromatin condensation; significantly alter the cell cycle arrest cells in the S phase, the cell membrane phospholipid phosphatidylserine valgus membrane structure changes; the anticancer mechanism inhibiting telomerase activity down Survivin protein, NF-κB and bcl-2 expression; the cordycepin role in human hepatoma cells, altering the cell cycle, typical apoptotic morphological changes, and Telomerase Activity induce apoptosis by down-regulating the expression of NF-κB. The experiments show that the Compound Cordyceps preparations days Lectra single component cordycepin different pharmacological mechanisms and a clear anti-tumor effect, shows the compound preparation of Cordyceps sinensis and cordycepin broad prospects for anti-cancer therapy applications.

Related Dissertations

  1. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  2. Effects of Elemene on Topoisomerase Ⅰ Andⅱof Human Hepatocarcinoma HepG-2 Cells,R735.7
  3. Biological Effects of Soluble CD40 Ligand on Leukemia HL-60 Cells and Its Mechanism,R733.7
  4. Effect of Bufalin on Proliferation and Induce Apoptosis of Vascular Endothelial Cells EAhy926,R285
  5. Effects of Dachengqi Decoction on the Expression Levels of Bcl-2 and Bax Gene of Smooth Muscle Cells of Small Intestine in Rat with Multiple Organ Dysfunction Syndrome,R285.5
  6. Effect of Clove Wei Ling Mixture on the Quantity of GAS and Bc1-2 in Rats Model of Chronic Atrophic Gastritis,R285.5
  7. Anti-cancer Effects of Tanshinone ⅡA Microemulsion for H22 Murine Hepatoma Cells Both in Vitro and in Vivo,R285.5
  8. GM-CSF in the Wound of Diabetic Rats BCL-2 Expression Levels,R644
  9. The Relationship Studying of Phosphatidylinositol-3-protein Kinase, Survivin Expression and Clinicopathologic of Rectal Carcinoma,R735.37
  10. The Studies to Anti-tumor Effect and Its Mechanisms of Nsaids Combined with Sodium Butyrate on Human Colon Cancer Cell Line HCT-116 in Vitro,R735.35
  11. Mangosteen genotoxicity studies with embryonic developmental toxicity,R285.5
  12. Full scrotum Ⅲ ° burn different repair methods on pig spermatogenic cells Bcl-2, Survivin protein expression,R644
  13. survivin and PTEN in epithelial ovarian tumors and its significance,R737.31
  14. YAP and Survivin in Endometrial Adenocarcinoma and estrogen receptor (ER) relationship,R737.33
  15. The Expression of YAP and Survivin in Ovarian Serous Papilary Cystadenocarinoma and Its Relationship with the Value of Serum CA125,R737.31
  16. Protection Effect of rhEPO to Apoptosis of Retinal Nerve Cells in High Glucose and Expression of Bcl-2 in Vitro,R966
  17. The Expression of Caspase-3 and Bcl-2 in Transitional Cell Carcinoma of Bladder and Their Significance,R737.14
  18. Research of Damage Efficiency by Livin/Survivin RNA Interference on Colorectal Cancer Cell Mediated by mPEGylated-chitosan Nanoparticles,R735.34
  19. Effect of Diallyl Disulfide on Cell Proliferation of Human Leukaemia K562 and Expression of Bcl-2, Bax, Survivin,R733.7
  20. Expression and Clinical Significance of Survivin and Annexin Ⅱ Protein in Epithelial Ovarian Tumor,R737.31
  21. The Effect of Total Phenolics from Abnormal Savda Munziq Combined with Chemotherapeutic Agents on HeLa Cells,R737.33

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile