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Expression and Significance of MTA1、nm23-H1 and E-cad in Esophageal Squamous Carcinoma

Author: WangJunPing
Tutor: ChenKuiSheng；ZhangGuoJun
School: Zhengzhou University
Course: Pathology and Pathophysiology
Keywords: Esophageal squamous cell carcinoma MTA1 nm23-H1 E-cad Infiltration Shift
CLC: R735.1
Type: Master's thesis
Year: 2007
Downloads: 99
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Abstract

In recent years, have cloned a variety of tumor invasion and metastasis related genes, including tumor metastasis gene (metastasis-associated gene 1, MTA1) study found that the signal transduction and gene regulation related throat metastasis-related genes. Studies have demonstrated that people MTA1 protein contains some genes that regulate the structure of proteins and binding proteins, in the process of invasion and metastasis of tumor cells, the the MTA1 gene product may in the signal transduction pathway plays an important role. At present, a large number of studies have shown that MTA1 with a variety of malignant invasion and metastasis. Metastasis suppressor gene (non-metastatic gene23-H1, nm23-H1) metastatic phenotype of a tumor suppressor related genes encoding product nucleoside diphosphate kinase (nucleoside diphosphate kinase, NDPK) This enzyme is involved in the body triphosphate nuclear glycosides generation, a subunit of the inactivation NDPK proportion imbalance, NTP produces insufficient, affect microtubule polymerization, resulting in chromosome fission and aneuploidy formation, promote tumor metastasis. Studies have shown that nm23-H1 gene by inhibiting cell of platelet-derived growth factor, insulin-like growth factor - Ⅰ reaction and affect the movement of the cells, thereby inhibiting tumor metastasis. The study confirmed that occur in a variety of high metastatic potential of tumors, nm23-H1 gene mutation, deletion or reduced expression, is closely related to that nm23-H1 and malignant metastatic potential. Cadherin (E-cadherin, E-cad) is a transmembrane protein isoforms in cell adhesion molecule family members, mainly present in the epithelial cells, the positive expression inhibit pain transfer functionality. E-cad expression decreased or absent, the interaction between the cell adhesion capacity decline, caused by the tumor cells were easily dispersed outward weeks invasive growth, and thus out of the primary tumor invasion and metastasis. The study found that the down-regulation of E-cadherin expression or lack of a malignant tumor behavior prompted E-cad expression levels as an indicator of tumor metastasis abilities. China is the world's esophageal cancer morbidity and mortality in countries with the highest ranking first in China, Henan Province, esophageal cancer morbidity and mortality, and the infiltration of the transfer is the main reason for the death of patients with esophageal cancer, so discuss the infiltration of the esophageal transfer mechanism has become one of the hot. For MTA1, nm23-H1 and E-cad expression in esophageal squamous cell carcinoma and the significance of the research, so far at home and abroad has not been reported in the literature. In this study, using immunohistochemical SP method were observed a high incidence of 49 cases of esophageal carcinoma, 30 cases of adjacent atypical hyperplasia and 49 cases of normal esophageal epithelium MTA1, nm23-H1 and E-cad protein expression three joint detection provides a theoretical foundation to further clarify the infiltration of esophageal cancer, the transfer mechanism, looking for an effective way to inhibit esophageal cancer invasion, metastasis, and improve the survival time of patients with esophageal cancer, reducing the mortality. The material tissue Series high incidence of esophageal Cancer Hospital, Anyang City, Henan Province, the First Affiliated Hospital of Zhengzhou University, September 2004 to November 2004 esophageal cancer fresh surgical resection specimens of 49 cases, each case surgical specimens in foci, paracancerous normal tissues of three drawn. The pathologically confirmed are squamous cell carcinoma. Select all 49 cases of cancer tissue, 30 cases of adjacent atypical hyperplasia and 49 cases of normal esophageal mucosal epithelial tissue as the observed object of this study. 49 patients with esophageal cancer specimens with or without lymph node metastasis is divided into: lymph node metastasis group (20 cases) and without lymph node metastasis (29 cases); divided into the depth of invasion: superficial muscle group (10 patients), deep myometrial group (15 cases), and adventitia group (24 cases); occurrence and development can be divided into: esophageal cancer group (49 cases), cancer adjacent atypical hyperplasia group (30 cases) and normal esophageal mucosa group (49 cases). All specimens in 10% formalin-fixed, paraffin-embedded, 4μm thick serial sections prepared immune histochemistry with. Method 1. Immunohistochemical SP method were 49 cases of esophageal carcinoma, 30 cases of adjacent atypical hyperplasia and 49 cases of normal esophageal epithelium MTA1, nm23-H1 and E-cad protein expression were detected. 2. Statistical analysis: All data were confirmed by SPSS11.0 software for statistical analysis. Between the positive rate was used to compare the X ~ 2 test (chi-square); relationship between the two variables the correlation analysis spearman level α = 0.05 for the test of significance level. Results 1. Normal esophageal epithelium MTA1 protein expression was 0% (0/49), atypical hyperplasia tissue MTA1 protein expression was 26.67% (8/30), esophageal tissue the MTA1 protein positive expression rate of 63.27% ( 31/49). The the MTA1 protein positive expression rate of cancer tissue and corresponding adjacent atypical hyperplastic tissue and normal esophageal mucosa epithelial tissue twenty-two compared and the differences were statistically significant (P <0.05). 2. Normal esophageal epithelium of nm23-H1 protein expression was 100% (49/49), atypical hyperplasia of nm23-H1 protein expression was 66.67% (20/30), esophageal carcinoma nm23-H1 protein-positive expression was 46.93% (23/49). The cancer tissue nm23-H1 protein expression and corresponding adjacent atypical hyperplastic tissue and normal esophageal mucosal epithelial tissue twenty-two compared the differences were statistically significant (P <0.05). 3. Esophageal carcinoma, atypical hyperplasia, and normal esophageal epithelium E-cad protein positive expression rate was 46.89% (22/49), 70% (21/30) and 100% (49/49), cancer tissue E compared to twenty-two-cad protein expression rate corresponding adjacent atypical hyperplastic tissue and normal esophageal mucosal epithelial tissue, the differences were statistically significant (P <0.05). 4.20 cases with lymph node metastasis of esophageal squamous cell carcinoma MTA1 protein positive expression rate was 80% (16/20), no lymph node metastasis in 29 cases of esophageal squamous cell carcinoma MTA1 protein expression was 51.72% (15/29), the two esophageal squamous cell the MTA1 protein positive expression rate between group difference was statistically significant (P <0.05). 5.20 cases with lymph node metastasis of esophageal squamous cell carcinoma nm23-H1 protein positive expression rate was 30% (6/20), lymph node metastasis, 29 cases of esophageal squamous cell carcinoma nm23-H1 protein expression was 62.07% (18 / 29) between the two groups of esophageal squamous cell carcinoma nm23-H1 protein expression rate differences (P <0.05). 6.20 cases with lymph node metastasis of esophageal squamous cell carcinoma of the E-cad protein expression was 25% (5/20), without lymph node metastasis in 29 cases of esophageal squamous cell carcinoma of E-cad protein positive expression rate was 58.62% (17 / 29), E-cad protein expression between the two groups of esophageal squamous cell carcinoma rate difference was significant (P <0.05). 7. Superficial myometrial invasion, deep myometrial squamous cell carcinoma of the esophagus and esophageal adventitia of MTA1 protein expression rates were: 40% (4/10), 46.67% (7/15) and 83.33% (20/24) infiltration of the outer membrane of esophageal squamous cell carcinoma MTA1 protein expression was significantly higher than the first two, and the difference was statistically significant (P <0.05), deep myometrial invasion of esophageal squamous cell carcinoma the MTA1 protein positive expression rate and superficial muscle layer group difference was not statistically significant (P> 0.05). 8. Superficial myometrial invasion, the deep myometrial esophageal squamous cell carcinoma and esophageal adventitia of nm23-H1 protein positive expression rate: 80% (8/10), 40% (6/15) and 37.5% (9/24 ), superficial myometrial invasion of esophageal squamous cell carcinoma of nm23-H1 protein expression was significantly higher than the latter two, the difference was statistically significant (P <0.05), and infiltration of deep myometrial esophageal squamous cell carcinoma nm23-H1 protein expression was compared with the adventitia difference was not statistically significant (P> 0.05). 9. Superficial myometrial invasion, the deep myometrial of esophageal squamous cell carcinoma and esophageal adventitia of E-cad protein positive expression rate: 90% (9/10), 46.67% (7/15) and 25% (6/24 ), superficial myometrial invasion of esophageal squamous cell carcinoma E-cad protein expression was significantly higher than the latter two, the difference was statistically significant (P <0.05), deep myometrial infiltration in esophageal squamous cell carcinoma of E-cad protein expression was compared with the adventitia difference was not statistically significant (P> 0.05). 10. MTA1, nm23-H1 and E-cad protein expression in esophageal squamous cell correlation by statistical analysis shows that between the former and the latter two were negatively correlated (P <0.01), and a positive correlation between the latter two ( P <0.01). Conclusion 1. Normal esophageal mucosal epithelial tissue the paraneoplastic atypical hyperplasia tissue and esophageal squamous cell carcinoma in the MTA1 protein expression rate increased successively, and normal esophageal epithelium, cancer adjacent atypical hyperplasia and carcinoma MTA1 protein expression between compared, the difference was statistically significant, indicating that MTA1 related with the development of esophageal squamous cell carcinoma. 2. MTA1 protein expression in esophageal squamous cell carcinoma metastasis group than those without metastasis group, the difference was significant, prompted MTA1 protein expression in esophageal squamous cell carcinoma metastasis. 3. Normal esophageal mucosal epithelial tissue, paraneoplastic atypical hyperplasia tissue and esophageal squamous cell carcinoma nm23-H1 protein, E-cad protein expression rate were decreased, and normal esophageal epithelium carcinoma adjacent atypical hyperplasia and carcinoma nm23-H1 protein, E-cad protein expression compared, the difference was statistically significant, indicating that nm23-H1, E-cad protein expression in esophageal squamous cell carcinoma related to the occurrence and development. 4. nm23-H1 and E-cad protein expression in esophageal squamous cell carcinoma metastasis group rate lower than the non-metastasis group, the difference was significant, suggesting that nm23-H1 and E-cad protein expression in esophageal squamous cell carcinoma metastasis. 5. The MTA1 protein positive expression rate in the different depth of invasion of esophageal squamous cell carcinoma, cancer cell infiltration depth deepened in the cancer tissue protein expression was gradually increased, and the invasion into the esophageal film cancerous tissue protein expression was significantly higher than the corresponding superficial myometrial invasion and deep myometrial tissue. Tip MTA1 protein expression in esophageal squamous cell infiltration. 6. nm23-H1 and E-cad protein expression in different depth of invasion of esophageal squamous cell carcinoma rate, with cancer infiltration depth deepened, cancer tissue protein expression was gradually reduced, and infiltration into the superficial muscle of the esophagus layer of cancerous tissue protein expression was significantly higher than the corresponding tissue deep myometrial invasion and the outer layer. Prompted nm23-H1, E-cadherin protein expression in esophageal squamous cell carcinoma infiltration. 7. MTA1, nm23-H1 and E-cad protein expression in esophageal squamous cell carcinoma in the statistical analysis, showed that all three may occur in esophageal squamous cell carcinoma, and infiltration, the transfer process from the synergies.

Expression and Significance of MTA1、nm23-H1 and E-cad in Esophageal Squamous Carcinoma

Abstract

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