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Anti-rejection Effect of Triptolide on Islet Allografts in a Murine Model

Author: CuiShiHua
Tutor: LiFei
School: Capital University of Medical Sciences
Course: Surgery
Keywords: Mice Type I diabetes Streptozotocin Islet Isolation Islet Transplantation Triptolide Immunosuppressive
CLC: R657.51
Type: Master's thesis
Year: 2007
Downloads: 83
Quote: 0
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Abstract

Chemical method, the purpose of establishment of type I diabetes in mice model. Method to C57BL / 6 mice by intraperitoneal injection of different doses of streptozotocin (of streptozotocin, STZ). Tails 2 to 6 days after the administration of blood collection method for the determination of non-fasting plasma glucose and body weight measurement. Blood glucose greater than 20mmol / L as the standard of success induced diabetic and diabetic mice was observed mortality. C57BL / 6 mice by intraperitoneal injection of STZ results to 150mg/kg, 200mg/kg and 225mg/kg dose. Administration at 2 days after the induction of diabetes ratios of 0%, 60% and 100%; 5 to 6 days were 60%, 100% and 100%. 225mg/kg dose group only one mouse died (mortality%). Induction of successful weight loss in mice, the decline STZ dose. Diabetic mice remained hyperglycemic state after two weeks of treatment by insulin. Conclusion intraperitoneal injection of STZ (225 mg / kg) is simple, safe and effective method of preparation of type I diabetes in mice model. Objective To establish stable mouse islet isolation and islet transplantation model. Methods BALB / c mice as donor. Common bile duct puncture pancreatic perfusion to Ⅴ collagenase solution, 37 ° C water bath still digest, purified islet cells Ficoll discontinuous density gradient method. STZ-induced C57BL / 6 diabetic mice as a receptor, in renal subcapsular implant 400 islet cells. Portable postoperative monitoring of blood sugar and body weight. 3 days after blood glucose below 10mmol / L as the transplant success criteria. After 7 days, the line charge nephrectomy in mice transplanted islets and monitor their blood glucose. The results, an average of each of BALB / c mice pancreatic 95 ± 12 (85 ~ 112) of pancreatic islet cells can be obtained. All diabetic C57BL / 6 mice after implantation of pancreatic islet cells, the first day of glucose were lower than 10mmol / L, and maintained until the first 11 days after surgery. Weight in a short-term decline in transplant recipients, and gradually increased, indicating that the diabetic state was corrected. 7 days after resection bearing transplanted islet kidneys, blood glucose increased again and is greater than 20 mmol / L, confirmed that the implanted islet function. Conclusion collagenase to Ⅴ digestion and Ficoll discontinuous density gradient purification to better islet yield can be obtained. Renal subcapsular islet transplantation for the treatment of diabetic mouse stable and effective. Objective To investigate the Triptolide anti-mouse islet transplantation rejection. Methods BALB / c mice as the donor, the separation of islet cells. STZ-induced diabetic mice of the C57BL / 6 as a receptor, renal subcapsular islet cell transplantation. Postoperative divided into treatment and control groups: the treatment group five days after intraperitoneal injection triptolide (50μg/kg) after the next day injection to 14 days after operation; control group received an equal volume of solvent (1% spit temperature of 80). Postoperative monitoring of blood glucose. Graft rejection is defined as a blood glucose twice greater than 20mmol / L. Results of the the islet graft Triptolide treatment group median survival time of 29.5 days (23 days to 30 days), while the control group islet graft median survival time was 14.0 days (13 days to 16 days), both significant difference (P lt; 0.0001). Conclusion Triptolide mouse islet allograft survival time can be extended by.

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