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Analysis of Longdong Wild Alfalfa Using Isozymes and SSR Markers

Author: ChenLiQiang
Tutor: ShiShangLi
School: Gansu Agricultural University
Course: Grassland
Keywords: Longdong wild alfalfa Isoenzyme Kinship SSR
CLC: S541.9
Type: Master's thesis
Year: 2010
Downloads: 50
Quote: 0
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Abstract


Distribution in Gansu Shimizu half damp the foot zone Longdong Wild Alfalfa (Medicago sativa L.cv.Qingshui) of the stronger drought, cold resistance and trampling ability, and has the ability of roots, branches, and therefore has the potential Fodder cultivation and good value of germplasm resources. In order to further the effective use of this resource, test using isozyme and SSR markers to analyze the genetic relationship between the Longdong wild alfalfa and cultivated alfalfa varieties. (1) Test peroxidase enzyme (POD), hydrogen peroxide enzyme (CAT), amylase (AMY), esterase (EST) and superoxide dismutase (EST) on Longdong wild alfalfa and other 37 the alfalfa varieties kinship, the results show that the tested materials showed a total of 41 enzymes with which 5 Longdong wild alfalfa characterized enzyme with. Longdong wild alfalfa and 37 other tested materials similar coefficients ranged from 0.585 to 0.976, far lower than the other tested materials similarity coefficient (0.861 to 0.919), cluster analysis showed that the test material in similarity coefficient of 0.662 can be clustered into two classes, which Longdong wild alfalfa clustered into a separate consistent its results principal component analysis. (2) test using SSR markers Longdong wild alfalfa and other 41 varieties of alfalfa kinship, 15 pairs of primers detected a total of 231 alleles per SSR primer pairs to detect the allele between 12 to 22, an average of 15.4, polymorphism information content (PIC) ranged from 0.846 to 0.938, an average of 0.909. The results showed that the average similarity coefficient (0.709) between Longdong wild alfalfa and 41 other tested materials is far lower than the other tested materials between the average similarity coefficient (0.720 to 0.831). Cluster analysis 42 material similarity coefficient 0.749 3, Longdong wild alfalfa clustered into one group alone, the results of principal component analysis results are basically the same. Isozyme and SSR markers that distant relationship between the Longdong wild alfalfa and other cultivated alfalfa varieties, which can be determined Longdong wild alfalfa as a relatively independent alfalfa germplasm resources to expand cultivation the genetic basis of the varieties. The test at the same time to determine the genetic relationship between Longdong wild alfalfa and alfalfa varieties tested, parent selection provides the basis for Alfalfa Introduction.

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CLC: > Agricultural Sciences > Crop > Fodder crops,pasture > Perennial legume > Other
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