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Study on Polysaccharide from Plants

Author: TianWei
Tutor: ZhangHaiXia
School: Lanzhou University
Course: Analytical Chemistry
Keywords: Lanzhou University Polysaccharide molecule Diabetic rats Crude polysaccharide Tea Polysaccharide Bitter gourd Master's degree thesis Anti-diabetic Tetrandrine Glucose
CLC: R284
Type: Master's thesis
Year: 2007
Downloads: 514
Quote: 1
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Abstract


This thesis first summarizes several plant polysaccharides and its anti - diabetic effects profile , and summary and discussion of the four plants ( tea , mushrooms , Anemarrhena , bitter gourd ) polysaccharide systems experimental research . Experimental section includes the following : 1 . Extraction, separation and purification of the four plant polysaccharides ; 2 . Four plant polysaccharides the physical and chemical nature of its structure ; 3 . The biological activity of four plant polysaccharide statistical differences : crude polysaccharides of the four plants insulin secretion in vitro experiments , the found only tea polysaccharides on glucose-induced insulin secretion . The tea polysaccharide has a regulatory role of insulin , when low-sugar , tea polysaccharides can enhance the secretion of insulin and high glucose can reduce the secretion of insulin . Other polysaccharides on insulin secretion no direct regulatory role. Three Plant crude polysaccharide ( tea , the mushrooms , Anemarrhena ) diabetic rats , the results show that tea , mushrooms treatment group , the control group , insulin levels and blood sugar are no significant differences , but Anemarrhena treatment group final serum insulin relative increase , reaching the level of the normal group , there may relieve the symptoms of diabetes . Bitter gourd polysaccharides in vitro anti- tumor effect , found that bitter gourd polysaccharide dose group MGC -803 , HEP- G2 , S180 three tumor cell lines have a certain inhibitory effect , but the effect of MGC-803 is best . On this basis , MGC-803 induced apoptosis of tumor cells and morphological observation made ??of bitter gourd polysaccharide . The experimental results show that the the bitter gourd crude polysaccharide by MGC-803 induced tumor cell apoptosis , inhibition of tumor cell growth and proliferation . Bitter gourd crude polysaccharides can be used as an adjunct to anti - MGC-803 tumor cells .

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