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The biocompatible material as a cell adhesion and growth of the templates, play an important role in the repair of cartilage tissue . Biological material cytotoxicity , cell adhesion properties , cell proliferation is an important indicator of the biocompatibility of materials . Polycaprolactone ( PCL ) and chitosan ( CS ) both have good biocompatibility , biodegradable and non-toxic biological materials , and widely in the biomedical field . PCL Ordering piled and folded , and forming an elongated crystallization region , the molecular chains in the amorphous region is interwoven semi - crystalline linear polymer , and thus a polycaprolactone having a good compression performance and mechanical relaxation and creep performance and having good flexibility . Precisely because of the semi-crystalline nature of the PCL , PCL does not have a good hydrophilicity . Chitosan and its derivatives due to their renewable , biocompatibility and structural variety of active groups , an amino group , an acetyl group, and a hydroxyl group , have good hydrophilic properties and biocompatibility , but its lack of the basic mechanics mechanical properties . High concentration of acetic acid as a co- solvent blend , both to form a homogeneous composite material , obtained by mixing the mixed material mechanical properties improved , especially PCL : CS mass ratio of 80% , the mechanical properties improved significantly . PCL : CS quality than 50% of its effect on cell adhesion and the pure chitosan no significant difference in cartilage cells cultured for 7 days , 14 days , after 28 days , cartilage cell proliferation significantly . This is because the PCL structure improves the mechanical properties of the material to maintain the 3D pore structure of the material , so that cartilage cells are able to intake from outside the discharge of nutrients and metabolites . 3D pore structure pure chitosan after immersed in PBS 2 weeks , it can not maintain its high porosity , the collapse of the structure and prevent cell material exchange with the outside world . The experiments using particle leaching NaCl ( diameter 200-300μm) as a pore-forming agent to obtain a porosity of 80 % of the pores of the voids of the porous scaffold material pore structure by SEM analysis , the high porosity of the cell adhesion, proliferation also plays an active role . The experimental program using MTT, toluidine blue , HE staining was evaluated .
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