Dissertation > Excellent graduate degree dissertation topics show

Inhibition of Hepatitis B Virus Expression in Mice by Rnai

Author: ShiChuan
Tutor: ShenGuanXin
School: Huazhong University of Science and Technology
Course: Immunity
Keywords: Hepatitis B virus RNA interference High pressure hydrodynamic tail vein injection
CLC: R512.62
Type: Master's thesis
Year: 2009
Downloads: 25
Quote: 0
Read: Download Dissertation

Abstract


The purpose of the hepatitis B virus (HBV) infection is the most important risk factors for human liver cirrhosis and hepatocellular carcinoma. In this study, a high-speed large number of injections containing HBV genome eukaryotic expression plasmid production HBV infection model via the tail vein, and injection of HBV siRNA, to detect interference effect, to lay the foundation for further study of its interference with immune effector mechanisms. Method 1 through the tail vein high-speed, large number of injections containing HBV genome eukaryotic expression plasmid production HBV infection model, the fourth day of treatment, respectively dosage effect relationship, the relationship between aging and the three imaging system observed plasmid pGenesil-hk GFP expression , verify that the model is successful. 2. Using time-resolved fluorescence assay siRNA interference mouse serum before and after the secretion of hepatitis B surface antigen (HBsAg) and e antigen (HBeAg), total RNA from liver cells using real-time quantitative PCR, immunohistochemical detection of liver tissue HBcAg. Results of a model of HBV infection in mice at high speed through the tail vein injection of HBV expression plasmid after four days, the quantitative detection of the HBsAg and HbeAg results show that pBlue-HBV gradually increased the amount of HBeAg rise; liver cells flow cytometry 2-9 days have varying degrees of expression, 3-5 days is the expression of the peak period. The results of in vivo imaging of mouse liver expression of green fluorescent normal mice, no fluorescence signal. Thus, we can confirm that the high-pressure water power established by injecting HBV infection in a mouse model, expression of HBV, serum liver? Detected HBsAg and HBeAg, and peak expression 3-5 days for about 10 days. 2.HBV siRNA interference effect Realtime PCR results show that siRNA group significantly inhibit the expression of HBsAg and HBeAg. Constructed the siRNA in vivo effectively inhibited at the mRNA level expression of HBV; Time-resolved fluorescence assay results suggest pGenesil-1 and pGenesil-2 group HBsAg and HBeAg expression decreased, indicating that siRNA can effectively inhibit the expression of HBV-associated antigen, further immunohistochemical detection results show that the undisturbed group showed more positive cells in pGenesil-1 and group 2 is almost no positive cells, siRNA can effectively inhibit the expression of HBcAg. Conclusions by high pressure hydrodynamic injection successfully established HBV infection in a mouse model, hepatic expression of HBV, HBsAg and HBeAg detected in the serum. The same method of injection HBV siRNA can effectively inhibit HBV mRNA and antigen expression, and the expression of HBcAg in liver cells, and laid the foundation for further in vivo targeting of interference with immune effector mechanisms in vivo.

Related Dissertations

  1. The Preliminary Studies on Characteristics of HCV Quasispecies Variation and Its Immune Escaping Mechanism,R392.1
  2. Expression Dynamics of Pheromone Binding Proteins and Expression Influences by Mating and Knockdown of Cryl in Spodoptera Exigua,S433.4
  3. Molecular Cloning, Mrna Expression and Rnai of Nadph-Cytochrome P450 Reductase Gene in Helicoverpa Armigera (Hǘbner),S435.622.3
  4. Studies on the Molecular Mechanism of Anti-HBV Effect of Green Tea Extract Epigallocatechin Gallate,R285
  5. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  6. Inhibition of Myostatin (MSTN) Expresstion in Primary Porcine Fetal Fibroblasts by Lentivector-mediated RNAi,S828
  7. Molecular Epidemiological Analysis of Duck Hepatitis B Virus in Cherry Valley Duck in Henan Province and Anti-duck Hepatitis B Virus Activities of FNC in Vivo,S858.32
  8. A Preliminary Study on the Regulation of Hepatoma Cell Biological Behavior by MCFP,R735.7
  9. The DNA Cloning and RNA Interference of Some Functional Genes in Laodelphax Striatellus (Fallen),S435.112.3
  10. The Effect of RNA Interference-mediated ERCC1 Gene on the Chemo-treatment Sensitivity of NSCLC in Vitro,R734.2
  11. Construction of an Full-Lengh Genomic cDNA Clone of Hev SAAS-JdY5,R346
  12. The Study of Inhibtion Effect by shRNA Expression Vector on Herpes Simplex Virus Type 2 UL54,R346
  13. Preliminary Study of Molecular Epidemiology on Hepatitis B among Certain Regions in Anhui Province,R512.62
  14. The Precise Measurement on Anti-HBc and Anti-HBe Detected by Chemiluminescent Microparticle Immunoassay,R512.6
  15. The Curative Effects of 72-week Entecavir Therapy for Patients with Decompensated Hepatitis B Virus-Related Cirrhosis,R512.62
  16. The Value of MELD Score and Serum Total Cholesterol in Predicting the Prognsis of Patients with Decompensated Liver Cirrhosis Caused by Hepatitis B Virus,R575.2
  17. Research and Analysis on Urine Proteomics in Hepatitis B Virus Associated Glomerulonephritis,R692.3
  18. The Influence of RNA Interference Suppression EphB4 Expression for the PANC-1 Cells of Pancreatic Cancer,R735.9
  19. The Characteristic of the Whole Hepatitis B Virus Gene, Construction of HBV Gene Eukaryotic Cell Expression Vector, and the Efficacy of Adefovir Plus Entecavir or Plus Lamivudine as Rescue Therapies in Chronic Hepatitis B Patients with Entecavir-resistant,R512.62
  20. Experimental Studies on Therapy of Lung Cancer by VEGF-C siRNA Mediated by Liposomes,R734.2
  21. The Functions of Chitinase Genes from Locusta Migratoria,S433.2

CLC: > Medicine, health > Internal Medicine > Infectious disease > Viral infections > Viral Hepatitis > Hepatitis B
© 2012 www.DissertationTopic.Net  Mobile