The purpose of patients with acute leukemia bone marrow microvessel density (MVD) was significantly higher, in order to better understand the MVD into the relationship between vascular factor and leukemia cell biological characteristics of the three, this project intends to study on patients with acute leukemia serum and leukemia cell lines cultured cleared of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) expression levels of VEGF-specific anti-oligodeoxynucleotides (ASODN) the impact on the growth of HL-60 cells. Collected 32 cases of acute leukemia patients and 10 healthy serum platelet and five kinds of leukemia cell line (HL-60, U937, NB4, JM and K562) culture supernatants were measured by sandwich ELISA for the concentrations of VEGF, bFGF, in order to eliminate interfere with the cell number of the measured values, the concentration of VEGF in the platelet count or the number of cells standardized, the final pg · mL -1 / 10 6 the PLT, pg · mL < sup> -1 / 10 5 cells as assessment indicators units; acute myeloid leukemia cell line HL-60 with different concentrations of VEGF ASODN using thiazole blue, ELISA method were measured target cell growth state and degree of VEGF secretion inhibition. Results ① acute leukemia patients and healthy individuals serum VEGF standardized values ??were (20.43 ± 8.32), (0.99 ± 0.27) pg · mL -1 / 10 6 PLT, a difference significant (P <0.05); ② addition to U937 outer, the remaining four leukemia cell lines culture supernatants were VEGF expression, concentration range (91.19 ~ 260.98) pg · mL -1 / 10 5 cells; ③ in 32 cases of acute leukemia patients, 14 cases detected expression of bFGF, the positive rate was 37.5%, significantly higher than that in healthy controls (10%) ( P <0.01); ④ in five leukemia cell lines, only U937 and K562 fine Fourth Military Medical University, master's degree thesis cellular supernatant detected bFGF expression; ⑤ 0.125 knife. 5, l, and 5umOIL'VEGFASODN role after 24h, HL-70 cell viability decreased to 77.8% ± 1.2% (P> 0 knife 5 VSCOfltrol), 61. l% disabilities 0.6% (P <0.05 |