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The Effect of EGB761 on Renal Tubular Epithelial Cells Apoptosis Induced by Hydrogen Peroxide Invitro

Author: WenHaiBin
Tutor: ChenJunNing
School: Guilin Medical College,
Course: Urology
Keywords: EGB761 Peroxide Renal tubular epithelial cells Apoptosis Survivin Caspase-3
CLC: R285.5
Type: Master's thesis
Year: 2010
Downloads: 58
Quote: 0
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Abstract


Objective hydrogen peroxide (H 2 O 2 ) induced tubular epithelial cells (NRK52E) of oxidative damage model, observation of Ginkgo biloba extract (EGb761) for large oxidative damage to the protective effect of rat renal tubular epithelial cells (NRK52E), research of EGB761 on renal tubular epithelial cell apoptosis, and to explore its mechanism of action. Methods Cultured normal renal tubular epithelial cells (NRK52E) divided into blank hydrogen peroxide-induced group, the different doses EGB761 protection group, select 400μmol / l the H 2 O 2 < / sub> 24 hours as the damage model dose and give different concentrations EGB761 protect, detect SOD value, to observe the cell damage, MTT method was used to observe the activity of cells, cell apoptosis was detected by flow cytometry and RT-PCR detection of survivin Caspase-3 mRNA expression. 1, compared with the control group, 100-400μmol / l range hydrogen peroxide (H 2 O 2 ) for renal tubular epithelial cells have obvious the injury, SOD value was significantly lower (P lt; 0.05), could be induced oxidative damage of renal tubular epithelial cells, MTT cell activity was reduced (P lt; 0.05), flow cytometry apoptosis rate increased (P lt; 0.05). RT-PCR observe the expression of Survivin mRNA did not change significantly (P GT; 0.05), Caspase-3 mRNA expression increased (P lt; 0.05) 2, compared with 400μmol / l hydrogen peroxide-induced group, SOD values ??have increased (P lt; 0.05), MTT shows cell activity increased (P lt; 0.05), flow cytometry shows the apoptosis rate decreased (P lt; 0.05 ), RT-PCR observed SurvivinmRNA of upregulated (P lt; 0.05), Caspase-3 mRNA downregulation (P lt; 0.05). Conclusion 1, hydrogen peroxide (H 2 O 2 ) in the range of 100-400μmol / l can induce apoptosis of renal tubular epithelial cells (NRK52E); EGB761 hydrogen peroxide (H 2 O 2 )-induced apoptosis has a protective effect in renal tubular epithelial cells (NRK52E); EGB761 may be through the following mechanisms. H 2 O 2 -induced oxidative damage of of NRK52E cells play a protective role; 1 EGB761 by improving the ability of the body against free radicals play a protective effect on renal ischemia-reperfusion in rats. ② EGB761 by the increase in the expression of Survivin of NRK52E cells, to cut Casepase-3 expression and inhibition of apoptosis of renal tubular epithelial cells, thereby reducing damage of NRK52E cells.

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