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Tea is one of the most popular beverages in the world , second only to the world's drinking water intake door to all beverage consumption in the second. Epidemiological studies have shown that certain tumors and tea consumption were negatively correlated . Tea polyphenols and their major component not only has anti-cancer effect , but also on tumor cells in vivo has a direct killing effect , but its anti-cancer mechanisms are not yet fully understood. Study found that many natural plant ingredients can induce apoptosis in tumor cells and make tumor cells cell cycle arrest , inhibition of tumor cell proliferation. Therefore, the tea extract or tea polyphenols may also be anti-cancer effect by inducing apoptosis of tumor cells and affect cell growth cycle to achieve. Objective To study the polyphenols induce apoptosis in lung cancer cells and its effect on cell cycle blockade. Methods Cultured human lung adenocarcinoma SPC-A1 cells , cells in logarithmic growth phase , adjusting the cell concentration to 10 5 / ml, inoculated into culture plates and cultured 24h, divided into control group and polyphenols drug group ( final concentration of polyphenols 50μg/ml, 100μg/ml, 150μg/ml, 200μg/ml, 250μg/ml, 300μg/ml), after dosing cultured 12h, 24h and 36h, MTT assay , agar sugar gel electrophoresis , transmission electron microscopy and flow cytometry polyphenols induce apoptosis and cell cycle arrest effect. Results MTT assay showed that tea polyphenols inhibit the proliferation of lung cancer cells , the inhibition was enhanced with time to 36h strongest inhibition . At a concentration of 50μg/ml ~ 150μg/ml range, inhibition was enhanced with the concentration ; when the concentration is greater than 150μg/ml , the inhibition increased with concentration but weakened. Agarose gel electrophoresis showed characteristic ladder , Transmission electron microscopy showed morphological changes of apoptosis , mitochondrial vacuolization , nuclear condensation, nuclear condensation . Flow cytometry polyphenols can find lung cancer cells were arrested in G 1 period to 24h, 150μg/ml blockade strongest (P <0.05). Conclusion polyphenols can induce lung cancer SPC-A1 cells apoptosis , and to cell cycle arrest at G 1 phase .
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