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Exploring the Essence of Fatty Liver’s Phlegm-Stasis Syndrome and Regulation of Molecular Mechanism from the Genetic Polymorphism of PPARγC161-T/CYPⅡE1

Author: HuChenBo
Tutor: ShiJunPing
School: Zhejiang University of Traditional Chinese Medicine
Course: Integrative Medicine
Keywords: PPARγC161 - T CYP Ⅱ E1 Gene polymorphism Fatty liver PBSS
CLC: R259
Type: Master's thesis
Year: 2010
Downloads: 46
Quote: 0
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Abstract


Objective To investigate the PPARγC161-T, CYP Ⅱ E1 gene polymorphism and fatty liver and fatty liver Phlegm proof of intrinsic relationship. By polymerase chain reaction-restriction fragment length polymorphism detected 183 cases of patients with fatty liver (phlegm syndrome in 79 cases, 69 cases of stasis, undocumented discernible n = 35) and 40 healthy controls were PPARγC161-T, CYP Ⅱ E1 gene polymorphism. 50 cases of fatty liver to do liver tissue puncture. Detection of all subjects BMI, waist circumference, blood pressure, determination of liver function (ALT, AST, GGT), TG, TC, HDL-C, LDL-C, UA, FINS, FBG, HOMA-IR, the use of logistic single factor and multivariate regression analysis independent risk factor for fatty liver. Results (1) fatty liver of waist circumference, BMI, SBP, FBG, HOMA-IR, TG, TC, UA, ALT, AST, GGT were higher statistically significant difference (P lt; 0.01) (2) the PPAR-γC161-T genotype and allele frequencies was no significant difference in the fatty liver group and the control group (P gt; 0.05) The PPAR-γC161-T genotype CC and CT / TT type FBG control group genotype CT / TT compared with the CC genotype (P lt; 0.05). The PPAR-γC161-T genotype and allele frequencies was no significant difference in the degree of fatty liver steatosis (p gt; 0.05) (3) CYP Ⅱ E1 genotype C1C2, C2C2 frequencies and allele the C2 frequency of fatty liver than in the control group increased (P lt; 0.05). CYP Ⅱ E1 genotype the C1C1 and C1C2/C2C2 type of fat the liver group C1C2/C2C2-GGT increased (P lt; 0.05) CYP Ⅱ E1 genotype and allele frequencies was no significant difference in the degree of fatty liver steatosis (P gt; 0.05) (4) the fatty liver sputum syndrome group, blood stasis syndrome group, undocumented discernible group among the three groups TG, TC, LDL-C were significantly different (P lt; 0.05), phlegm syndrome group TG, TC (P LT; 0.01), LDL-C (P lt; 0.05) is higher than without a discernible group, phlegm syndrome group TG (P lt; 0.01), TC, LDL-C (P lt; 0.05) higher than without a discernible group. Genotype and allele frequencies of the the the PPAR-γC161-T phlegm syndrome group of fatty liver, blood stasis syndrome group, undocumented discernible group than the control group no significant difference in the distribution. CYP Ⅱ E1 genotype C1C2, C2C2 frequency and allele C2 frequency phlegm syndrome group of fatty liver, blood stasis syndrome group, undocumented discernible group compared with the control group increased (P lt; 0.05) (5) Logistic regression analysis showed that waist circumference, TG, ALT role of the regression model was statistically significant (P lt; 0.05), the risk factors of fatty liver. Conclusion (1) CYP Ⅱ E1 gene polymorphism with fatty liver lipid peroxidation is closely related, closely related to the allele C2 and fatty liver disease. (2) lipid metabolism and lipid metabolism disorders caused by internal and external factors, lipid levels and fatty liver phlegm and blood stasis syndrome are closely related. (3) CYP Ⅱ E1 gene polymorphism the PBSS waiting fatty liver formation and the transformation of phlegm and blood stasis syndrome plays a decisive role. (4) waist circumference, hyperlipidemia, alanine aminotransferase increased risk factor for fatty liver.

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