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Antimicrobial Activities and Chemical Compositions of Liposoluble Compounds in Some Microalgae

Author: JiangHongXia
Tutor: ZhengZuo
School: Fujian Normal University
Course: Botany
Keywords: Microalgae Fat-soluble compounds Column chromatography on silica gel Eluted fractions Antibacterial activity
CLC: Q946
Type: Master's thesis
Year: 2003
Downloads: 356
Quote: 1
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Abstract


Using the disk diffusion method on the 12 species of marine and freshwater microalgae of a methanol: toluene (3:1) extracts for antibacterial activity screening results show that most of microalgae extract shows the different degrees of antimicrobial activity , wherein the protein core ball algae tower alga Synechococcus and Pseudomonas extract has strong antibacterial activity ; methanol : toluene ( 3:1 ) mixture of ether and acetone extraction effect of these six kinds of the 12 species of microalgae compare experimental results show that the extraction of methanol : toluene ( 3:1 ) mixture better . Chlorella pyrenoidosa , tower alga , Pseudomonas Synechococcus and Porphyridium cruentum microalgae by the Soxhlet and silica gel column chromatography to separate the different components , respectively, carried out the the antibacterial experiments and chemical composition analysis , the results show that the crude fat antimicrobial activity of Chlorella pyrenoidosa generally greater than each separate component ; tower alga and Pseudomonas Synechococcus , the benzene elution of the antibacterial activity of the component is generally higher than the other two components and antibacterial activity of crude fat ; the Porphyridium cruentum ethanol component the sweet potato summer Plague original bacteria , Staphylococcus aureus , and Blast A 15 relatively strong inhibitory activity ; algae petroleum ether eluted fractions almost no antibacterial activity. Strong inhibition of microalgae fat-soluble compounds tested three kinds of plant pathogens . Four microalgae petroleum ether and benzene eluted fractions were isolated and identified by GC-MS ( color - MS ) method , the four microalgae petroleum ether eluted fractions mainly alkanes , they almost no antibacterial activity; benzene elution constituents in complex , wherein the antimicrobial compound is needed to further separation and identification ; the four microalgae ethanol by GC elution fractions the separation, identification , found that fatty acid compound described mainly fatty acid compounds have a strong antibacterial activity . Shimadzu UV - 3000 UV - visible spectrophotometer scanning analysis showed that the fat-soluble pigment compounds in microalgae for chlorophyll alpha , beta - carotene and antibacterial activity of an orange-red pigments , pigment compounds needs to be further research . Four kinds of microalgae fatty acid composition and content analysis showed that four microalgae contain high content of high unsaturated fatty acids , higher medical and health value .

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