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This paper was about the quality assessment of Gegenqinlian preparation, which is composed of Radix Puerariae, Radix Scutellariae, Rhizoma Coptidis, Radix Glycyrrhizae.The preparation were identified by TLC (Thin Layer Chromatography), from which we can see that the spots were clear and that the recurrence was as expected. Radix Puerariae was detected by Silca gel-G, the mobile phase consisted of chloroform-methanol (7:3) and detection was performed at 365nm. Radix Scutellariae was examined by Silca gel-G, the mobile phase was ethyl acetate-butanone-formic acid-water (5:3:1:1). Rhizorha Coptidis was examined in the same way as Radix Puerariae, and the detection wavelength was 365nm. For the Radix Glycyrrhizae, the mobile phase was petroleum ether(60-90℃)-benzene-ethyl acetate-acetic acid glacial( 1:2:1:0.2), and the detection wavelength was 254nm.A reversed-phase HPLC method was established to determine the content of puerarin, baicalin, berberine and glycyrrhizic acid in Gegenqinlian preparation. Puerarin was analyzed by a Kromasic ODS column. The mobilephase consisted of methanol-water (30:70, v/v) and detection was performed at 254 nm. The results showed the calibration curve was linear in the range of 8.0~48 u,g-mL"’ and correlation coefficient was 0.9999(n=6). The average recoveries of tablet,decoction,capsule and micropill were 97.4%(RSD=1.3%), 96.7%(RSD=1.2%),97.3%(RSD=1.3%)and99.5% (RSD=1.1%) , respectively. Baicalin wasanalyzed by a Kromasic ODS column. The mobile phase consisted of methanol-water-phosphoric acid (52:48:0.2, v/v) and detection was performed at 280 nm. The results showed the calibration curve was linear in the range of 16-48 ug mL-1 and correlation coefficient was 0.999 l(n=5). The average recoveries of tablet, decoction, capsule and micropill were 99.0%(RSD=0.97%), 96.8%(RSD=1.1%), 97.3% (RSD=1.3%) and 99.5% (RSD=1.1%) , respectively. Berberine was analyzed by a Kromasic ODS column.The mobile phase consisted of methanol-water-triethylamine (70:30:1, v/v) and detection was performed at 254 nm. The results showed the calibration curve was linear in the range of 14-84 ug mL-1 and correlation coefficient was 0.9993(n=6). The average recoveries of tablet, decoction, capsule and micropill were 97.1%(RSD-1.1%), 97.2%(RSD=1.2%), 99.6%(RSD=1.3%) and 96.2%(RSD=1.4%), respectively. Glycyrrhizic acid was analyzed by a Kromasic ODS column. The mobile phase was methanol-water-36%acetic acid glacial (72:28:1, v/v) and detection wavelength was 254 nm. The results showed the calibration curve was linear in the range of 12.5 - 62.5 ug mL-1 and correlation coefficient was 0.9990(n=5). The average recoveries of tablet, decoction, capsule and micropill were 96.8%(RSD=1.5), 96.7%(RSD=1.4%),96.7%(RSD=1.3%) and 97.5%(RSD=1.2%) , respectively. From these data , we came to the conclusion that this method was simple, sensitive and reproducible and it can be helpful for the quality assessment of Gegenqinlian preparation.Using 3D-HPLC, we got further quality differentiation of these preparation. The chromatography column was Shim-pack VP-ODS, the mobile phase was methanol-water, which flowed by linearity grads. And the detection was performed set from 240nm to 370nm. The results proved that this way to identify crude drugs was advanced, sensitive, applicable, visual and convenient.The research object was the commonly used tablet of Gegenqinlian preparation, which was purchased 10 batches of the same pharmaceutical company, and studied on the quality assessment of this preparation. In the meantime, the 70% methanol extract of Gegenqinlian tablets was analyzed about the fingerprint spectrum by RP-HPLC, and 14 constituents of these were separated. These data suggested that there were some essential chemical constituents in this preparation and provided the basis for the quality assessment of this preparation.
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