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Experimental Study on the Effect of Radices Puerarire Total Flavone on Nitric Oxide System and Some Biochemistry Index in Blood of Training Rats

Author: XuYuE
Tutor: XiongZhengYing
School: Shaanxi Normal University
Course: Human Movement Science
Keywords: Pueraria lobata flavone Nitric oxide Nitric oxide synthase Inducible nitric oxide enzyme Liver glycogen Muscle glycogen Serum enzyme
CLC: G804.7
Type: Master's thesis
Year: 2002
Downloads: 235
Quote: 0
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Abstract

Chinese medicine Pueraria legume dry kudzu or kudzu root, commonly used in Chinese medicine Xin Liang relieving drugs. The function the Sun Jieji Touzhen antidiarrheal arrests restless thirst. Indications typhoid headache warm items strong, Fanre Diabetes, diarrhea, dysentery, typhus impervious, high blood pressure, angina, deafness. Pueraria in the main active ingredient puerarin for Pueraria Flavonid, daidzein, daidzin. Modern medicine by the chemical, pharmacological and clinical studies on Pueraria prove the Pueraria Flavonid has many therapeutic efficacy. Pueraria clear expansion of the role of the coronary blood vessels, brain blood vessels and improve microcirculation, reduce blood pressure, heart rate and myocardial oxygen consumption; also adopted anti-platelet aggregation, promotes endothelial cell function and regulation of vasoactive substances, protection of ischemia-reperfusion myocardial reduce myocardial infarct size; puerarin total flavonoids inhibit adrenaline activation of adenylate cyclase, β-receptor blocking effects; the Pueraria Flavonid has hypoglycemic effect, and can significantly improve the alloxan glucose tolerance; Pueraria lobata flavone can increase bone mineral density and increased bone strength, thereby delaying aging; improve the D-galactose induced aging mice learning and memory abilities; puerarin total flavonoids have anti-mutagenic, anti-teratogenic, anti-carcinogenic role. Pueraria use safe range, no significant side effects. Nitric oxide (NO) is a simple chemical structure of the particular small molecules, lively nature. The main places in vivo synthesis of nitric oxide in endothelial cells, airway cells, macrophages, nerve cells, neutrophils, liver cells, some tumor cells can also be nitric oxide synthesis. Nitric oxide role can regulate vascular tone is mainly to maintain the blood pressure is stable, maintain the integrity of the vascular endothelium, and prevent platelet aggregation; nitric oxide can dilate the trachea and bronchi; nitric oxide in the nervous system is considered and learning memory is closely related, is one of the patterns of neurons in learning and memory mechanisms; physiological role of nitric oxide in the immune system: nitric oxide macrophages play to kill target cells effect messenger molecule. However, nitric oxide can damage normal cells and tissue involved in autoimmune tissue injury. Nitric oxide is generated by nitric oxide synthase (NOS) catalysis. Animal vascular endothelial cell nitric oxide synthase There are two types, one is the continuous synthesis of nitric oxide synthase by vascular endothelial cells and nerve cells, called constitutive nitric oxide synthase (cNOS), another NOS is inducible NOS (iNOS), iNOS is not expressed in the physiological state, its expression need some cytokines induced. Usually physiological dose of nitric oxide produced by cNOS having a physiological adjustment function; large doses of nitric oxide produced by iNOS have cytotoxic effects. Studies have shown that nitric oxide was significantly increased in the serum or muscle tissue after exercise, the body good. This study investigated the supplement Pueraria of total flavonoids sports rats athletic ability and change of nitric oxide system, and provide a theoretical basis for sports nutrition food and health food will Pueraria. The experiment used 30 male SD rats, weight 1809 seven, two months of age, adaptability feeding 3d after the rats were randomly divided into the quiet control group (A), high-intensity endurance training group (B), high-intensity endurance training ten medication group (C). Rats in each group free water and basic feed intake: medication group were gavage administration, drug use cold soak extraction Pueraria lobata flavone suspension system. Once a day orally. Group A quiet feeding group B and group C animal treadmill the first adaptive training for five weeks, and then 2 Zhou Daqiang degree of endurance training. U weighing 8 weeks in the groin vein after mild anesthetic blood collection, preparation of serum, and then off to the marrow were sacrificed immediately take the heart, liver, kidney, brain, skeletal muscle spare. Determination of physiological indicators: monoamine oxidase (**): Nitric oxide (* O plant total nitric oxide synthase (total NOS); inducible nitric oxide enzymes (iNOS); muscle glycogen and liver glycogen. Passing of this experimental research the following conclusions can be drawn: supplement Pueraria lobata flavone content of nitric oxide (NO) in the movement of rats in vivo myocardial tissue and liver tissue increased to improve the movement of rat heart and liver vasoconstriction state, so that the vasodilation increased blood supply; myocardial tissue nitric oxide synthase activity higher than the exercise group; liver tissue, brain and kidney tissue nitric oxide synthase activity is lower than the exercise group, may be on the body of a kinds of protective role: the myocardial tissue, brain and kidney tissue of inducible nitric oxide activity than the exercise group increased: the activity of inducible nitric oxide in the liver tissue lower than the exercise group; may be the movement of rats vivo monoamine oxidase (MAO) activity increased; enables the movement of rats in vivo liver glycogen and muscle glycogen content increased; supplement Pueraria lobata flavone can exercise rats some serum enzyme activity corresponding change.

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