Dissertation > Excellent graduate degree dissertation topics show

Characterization of Corn Lectin and Application to Screen of Growth-promoting Bacteria from Corn Rhizosphere

Author: LiuChi
Tutor: WuKun
School: Henan Agricultural University
Course: Microbiology
Keywords: Corn Lectin Characterization Affinity strains Identification
CLC: S513
Type: Master's thesis
Year: 2011
Downloads: 8
Quote: 0
Read: Download Dissertation


Plant Lectin is defined as a kind of sugar-protein which is composed of at least one uncatalysed region binded reversibly to a specific mono- or oligosaccharide. Different lectin was able to identify specially relative microorganisms due to its unique sugar-binding activity.Nitrogen-fixation bacteria, phosphate-releasing bacteria and potassium-releasing bacteria had been isolated from corn rhizosphere by Ashby medium, phosphate-releasing bacteria medium and potassium-releasing bacteria medium, respectively. All strains were re-screened by corn lectin 1abeled with fluorescein isothiocyanate.As a result,4 affinity strains were obtained and identified from cell and colony shape, physiological characters and 16S rDNA.Corn Lectin was extracted from corn seed powder by PBS buffer solution, prcipitated with (NH4)2SO4, purified through Sepharose 4B and lyophilized. The extraction rate of Corn Lectin was 0.154mg?g. The purified Corn Lectin was labeled with FITC by Marshall’s method and the agglutinating titre was 1:16.This paper had studied the characterization of Corn Lectin in the following 4 aspects: (1) The corn lectin displayed the maximum agglutination activity with erythrocytes of rabbit but no agglutinating activity with A, B, AB, O types of human blood. (2) The lectin showed a low tolerance to heat treatment. It was stable at below 40℃. The agglutinating activity was a little lower at 50℃, but totally lost at above 60℃for 10 min. (3) The agglutinating activity of corn lectin would be showed at pH 3.0-10.0, and more potent at pH 8.0-9.0. Therefore, its lectin activity was inactivity at pH above 10.0. (4) EDTA, K+, Ca2+, Mg2+ had no effect on the agglutinating activity of corn lectin, whereas its agglutinating activity could be inhibited by Fe3+, Mn2+. The agglutinating titre was remain 1:16 after labeled by FITC by Marshall’s method. In the experiment 15 strains of nitrogen-fixation bacteria, 30 strains of phosphate-releasing bacteria and 26 strains of potassium-releasing bacteria were isolated from corn rhizosphere. All strains were re-screened by corn lectin 1abeled with FITC.As a result,4 affinity strains were obtained, including 1 strain of nitrogen-fixation bacteria, named as N12; 2 strains of phosphate-releasing bacteria, named as P07, P23; 1 strain of potassium-releasing bacteria, named as K19. These strains were belong to Azotobacter, Bacillus, Pseudomonas and Bacillus, respectively.With the development of modern agriculture, excessive chemical fertilizer leads to lots of serious problems such as soil deterioration, environmental pollution and so on. Microbial fertilizer is high-efficient, low-cost and non-pollution. In this paper, the affinity strains were selected by corn lectin labeled with FITC. All these strains can be used as the original strains of microbial fertilizer especially for corn.Therefore, the development of microbial fertilizer which can reduce the amout of chemical fertilizer could solve the problems of environmental pollution and eventually benefit the quality of produce and environment.

Related Dissertations

  1. Temperature Drift Modeling and Compensation of Fiber-Optic Gyroscopes,V241.5
  2. Simulation and Analysis about Switched Reluctance Generator Power Supply System,TM31
  3. Research on Vector Control System of Induction Motor Based on DSP,TM346
  4. Design and Simulation of UHF RFID System Based on the Protocol of EPC C1G2,TP391.44
  5. The Software Component Modeling Method Based on Feature and Its Application in VMI Management System,TP311.52
  6. Research on the Control and Remedy of Nanopositioning Stage Drived by Piezo Material Lead Zirconate Titanate,TP273
  7. Aerial Target Anti-interference Recognition and Tracking System,TN215
  8. Studies on Synthesis, Characterization and Properties of Fe-V Co-doped TiO2 Catalyst,O614.411
  9. The Study on Rhinoceros Horn and Its Imitation,TS932.2
  10. Study on Antioxidant Components of Tibetan Medicine Sanguo Tang San,R29
  11. Health-based and Ecological Risk Assessment of Contaminated Sites,X820.4
  12. Over-voltage Identification of Transmission Line Based on Hilbert-Huang Transformation,TM866
  13. Study of Synchronous Generator Excitation Control Based on Neural Network Identification,TM31
  14. Survey in Nanjing and Analysis of the Mitochondrial Genome of Frankliniella Occidentalis (Pergande),S433
  15. Research on Peer-to-Peer Traffic Identification Algorithm Based on Cluster Analysis,TP393.02
  16. Preliminary Research on Characterization of Thermophilic Protease Pasey and Helicase Htc16,Q814
  17. Animal products quality safety and security regulation of RFID systems,TS201.6
  18. The Identificationand Rapdanalysis of Wheat Root Lesion Pathogens in Henan Province,S435.121
  19. Identification on the Species and Pathotypes of Four Cereal Cyst Nematode Populations from Western and Northern Henan,southern Hebei and Evaluation of Wheat Cultivars Resistance to Two Populations,S435.121
  20. Isolation and Characterization of Carbendazim-Degrading Strains, Cloning and Expression of the mheI Gene,X172

CLC: > Agricultural Sciences > Crop > Cereal crops > Corn ( maize )
© 2012 www.DissertationTopic.Net  Mobile