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The Biosorption Characteristic of Cd~(2+) on Candida Untilis under Negative Pressure Cavitation from Polluted Water

Author: CaoYuJie
Tutor: ZuYuanGang
School: Northeast Forestry University
Course: Botany
Keywords: Utilis yeast Cd2 Vacuum cavitation biosorption Adsorption mechanism Langmuir isotherm model Freundlich adsorption model
CLC: X172
Type: Master's thesis
Year: 2007
Downloads: 95
Quote: 1
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Abstract


This paper is based on the national \to explore an efficient, new heavy metal wastewater biosorption new technology and new methods. By comparing the traditional Shaker cavitation vacuum adsorption adsorption and adsorption rate, unit adsorption rate, adsorption rate, and the use of advanced micro-observation by means of the yeast Candida utilis adsorption Cd 2 of the process the physiological and ecological aspects of monitoring, its adsorption mechanism was more in-depth study and model fit by adsorption on two different ways an advantage compared adsorption. The paper won the main conclusions are: (1) to obtain the optimum adsorption conditions: pH is the most economical Cd 2 solution natural pH = 4.8; most suitable for adsorption experiments Cd 2 < / sup> concentration range of 20 ~ 200mg · L -1 between; Cd 2 concentration of 50mg · L -1 as the standard, the most suitable for adsorption experiments utilis yeast concentration of 3g · L -1 ; optimum adsorption duration of 60min. (2) yeast cells using BCECF staining reagent, the use of confocal microscopy the pH of the yeast cells was determined that, regardless of the shaking cavitation vacuum adsorption or absorption, the intracellular pH value with time prolonged elevated from acidic to slightly alkaline, the pH may be elevated this yeast to environmental stress some characterization. (3) using Phen Green SK (dipotassium salt) reagent adsorbed yeast cells were stained using laser confocal yeast intracellular Cd 2 of the distribution. Speculated that cavitation vacuum adsorption-absorption rate of the main possible shaker negative pressure cavitation of the special power, force Cd 2 adsorbent into the inside, thereby increasing the absorption rate . (4) by atomic force microscopy shaker vacuum cavitation adsorption and adsorption yeast samples after 60min observe that, shaking the cell surface after adsorption appear a little wrinkled, and vacuum cavitation adsorbed on the cell surface roughness increased, This may be excited by the negative pressure of the ultrasonic cavitation generated by the shearing forces. (5) on the production of Candida biosorption Cd 2 process of the Langmuir isotherm model simulations, were given a shaker shaker adsorption and adsorption and adsorption vacuum cavitation maximum adsorption capacities the q max 17.36mg / g and 17.70mg / g, i.e. negative potential cavitation adsorption-adsorbed slightly shaking. The corresponding K d 0.0132 and 0.0497, respectively, i.e. under the same adsorption conditions, cavitation vacuum adsorption-absorption rate is higher than shaker. (6) on the production of Candida biosorption Cd 2 Freundlich adsorption process of the model simulations, were given a shaker vacuum cavitation adsorption and adsorption characteristic constants Kf were 1.61mg / g and 3.70mg / g, both negative pressure cavitation adsorption adsorption adsorption potential than shaker. The corresponding n 2.92 and 3.95 respectively, i.e. under the same adsorption conditions, the negative pressure adsorption with respect to cavitation more likely to occur shaker adsorption adsorption. The completion of this thesis reveals the vacuum cavitation technology works biosorption process parameters as well as the yeast Candida utilis biosorption Cd 2 to strengthen the role, initially proved negative pressure cavitation biological Adsorption is an effective new methods. In this paper, a large number of experimental analysis, summed up a complete set of technical data for future biological assay vacuum cavitation industrialized pollution control work has laid a solid foundation.

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