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Case-Control Study on the Associations of Genetic Polymorphisms of ER and PR with the Risk of Breast Cancer

Author: JinYaLi
Tutor: ShenYuePing
School: Suzhou University
Course: Epidemiology and Biostatistics,
Keywords: Breast cancer Case - control study Estrogen receptor (ER) Progesterone receptor (PR) ER codon325 is PR 331G / A Gene polymorphism
CLC: R737.9
Type: Master's thesis
Year: 2007
Downloads: 144
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Abstract

[Background] the past 20 years, China's female breast cancer incidence is rising rapidly and the patient's age was young trend has become one of the most important malignant threat to women's health and life. From a new perspective affects the risk factors for breast cancer, has become a cause of breast cancer the field of exploration and prevention urgent need to address the issue. Studies have shown that estrogen and progesterone and its related factors closely related to breast cancer, estrogen receptor (ER, estrogen receptor), progesterone receptor (PR, progestin receptor) gene polymorphism may be estrogen and progesterone cause breast an important factor in cancer carcinogenic chain. [Objective] The purpose of this study is to explore the estrogen, progesterone receptor gene polymorphism (ER codon325, PR 331G / A) whether to raise the risk of breast cancer of the female population in China; between two loci and in-depth study and their gene - gene and gene - Setting the combined effects of environmental factors in the etiology of breast cancer research and screening of high-risk populations cumulative item. [Methods] In this study, cases for January 1, 2005 to 1 July 2006 in General Surgery, Changzhou First People's Hospital Breast Branch and the First People's Hospital of Suzhou new pathologically confirmed breast cancer cases, aged 25 to 70 years old. Controls frequency matched by age Changzhou and Suzhou City to participate in the examination of women's diseases of women suffering from cancer. Structured questionnaire survey study age, menstrual history, reproductive history, family history, lifestyle and diet history of epidemiological data collected in 3 ml of venous blood. The effective sample size of 420 patients (cases of 206 patients in the control of 214 cases). With the quantitative PCR ER codon325, PR 331G / A genotype. Quality control measures include 10% of the experimental sample repeat the experiment, 10 representative sample sequencing, double over the data entry and proofreading. Relative risk estimate OR and 95% CI estimated strength of association study of factors associated with breast cancer. The main statistical methods for the chi-square test, Hardy-Weinberg equilibrium test, Logistic regression and Rothman risk factors combined effects of additive effect test. Using the statistical analysis software SAS8.1 (SAS Institute, Cary, NC USA). [Results] The study found that early age at menarche, early age of first sexual life, history of taking oral contraceptives, family history of breast cancer, family history of benign breast disease, history of benign breast disease, history of passive smoking, drinking history, exposure to pesticides history, the annual intake of pork and salted high may be a risk factor for breast cancer in women; the protective factors tea Years, adolescence and nearly 10 years of regular exercise, fresh milk, fresh beans and fruit intake amount. Found no history of taking hormone drugs, ended the milk injection or a history of drug use, height, weight, BMI statistically significant association with breast cancer. ER codon325 polymorphic loci CC, CG and GG genotype frequency distribution in the general population of women, respectively, to 25.23%, 53.27%, 21.50%, PR 331G / A multi-polymorphic loci GA and GG genotypes in women The population distribution of frequencies were 92.99%, 7.01%, respectively. A single gene locus and breast cancer: study found no significant association between the ER codon325 polymorphic loci associated with breast cancer, compared with the CC, CG and GG genotypes adjusted OR was 0.99 (95% CI: 0.61- 1.61) and 1.13 (95% CI :0.62-2 .04). The study found that PR 331G / A polymorphism showed a trend of increased risk of breast cancer, compared with GA, the GG genotype OR = 1.51 (95% CI :0.74-3 .07), but not significant. On the joint genetic risk estimates, the study found that ER codon325, PR 331G / A joint polymorphism with increased risk of breast cancer trends, but not significant. CC and CG of the ER codon325 gene and PR 331G / A the GA genotype combination of the reference group, the ER codon325 gene CC and CG, PR 331G / A GG genotype adjusted OR of 1.80 (95% CI: 0.76 -4.30) ER codon325 GG, PR 331G / A GA group adjusted OR of 1.19 (95% CI :0.71-2 .01), ER codon325 GG, PR 331G / A GG group adjusted OR of 1.16 (95 % CI :0.34-3 .94). The study found no increased risk of breast cancer with the mutation sites in a growing trend (trend test, P = 0.4090). The combined effects of genetic and environmental risk factors analysis prompted, ER codon325 polymorphism may be associated with spontaneous abortion are the combined effects of the increased risk of breast cancer, close to the level of significance (P = 0.0588). With a history of spontaneous abortion and women carrying the CC genotype compared PR 331G / A polymorphism and found no history of spontaneous abortion and carry CG and GG genotype women OR 3.57 (95% CI :1.21-10 .52); environmental risk factors for breast cancer risk dangerous combination. [Conclusion] 1. Early age at menarche and history of taking oral contraceptives to women with breast cancer risk factors; a tea years long protective factors. 2. There was no significant correlation between ER codon325 polymorphism and breast cancer; 3. Weaker correlation may exist between the PR 331G / A and breast cancer; 4. ER codon325 PR 331G / A between there may be increased risk of breast cancer combined effects; 5. Between the the ER codon325 polymorphism and spontaneous abortion increase the risk of breast cancer combined effects close to the level of significance.

Case-Control Study on the Associations of Genetic Polymorphisms of ER and PR with the Risk of Breast Cancer

Abstract

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