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The North Asarum important herbs of the Northeast region, and the active ingredient methyl eugenol in the roots and rhizomes on bud development and differentiation status affects the accumulation of the active ingredient. In this study, the field field observation mirror entity anatomical, optical microscopy, scanning electric microscopy techniques, North Asarum bud characteristics and differentiation and size of the spores occurred male and female gametophyte development, etc., carried out a systematic study, and the results are as follows: 1. According to bud within produce leaf number of North Asarum bud into the germ (non-leaf), buds (with one leaf) and mixed Bud (basic two true leaves) 3 types. 2 flower bud differentiation starting from the end of June to early August, the end of last month, the case of the summer and differentiation type. The entire flower bud differentiation process including undifferentiated period beginning differentiation period, the five periods of the perianth primordium differentiation stage of stamen, pistil primordium. ABA content in North Asarum flower bud differentiation process reduce inhibit flower bud differentiation promote the flower bud differentiation GA and IAA content increased the reduced ABA / IAA and ABA / GA value is not conducive to the conduct of the flower bud differentiation. . The North asarum with 12 stamens, up and down two six alternating. Small meiosis belong to the same time, type, the microspore tetrad arrangement most Tetrahedral small number of cross-shaped. Anther wall development of basic glandular tapetum mononucleate pollen developmental stage, tapetum within the wall of a large number of spherical body Ubbelohde tangential. The mature pollen 2 - cell type, flat ball, no significant germination hole outer wall surface was wavy tuberculate-like protrusions. 5. Pistil 6, carpel 6, Room 6 ovary, axile, stigma cristate, hollow style, ovules the Born carpellary belly online, with Lacoste's down the ovule thick nucellus , micropyle. Embryo sac development type Polygonum type, and order of the beads disk structure is retained until the seeds are ripe. 6.6 in mid to late mature fruit, black viscous subsidiary of material covering the concave surface of the skin at the mature seed, the seed embryo undifferentiated fully develop to the stage of the original embryo. The endosperm development belongs karyotype, about 50d after sowing the seed, the seed embryo after globular embryo, heart-shaped embryo, torpedo-type embryos until develop into mature embryos with full cotyledon. General effect 7. 300ppm gibberellin (GA) can significantly increase the rate of seed germination, and hypocotyl 8d break through the seed coat in advance; cytokinin (6-BA) role is not obvious ,6-BA and GA used in combination ; cotyledons after GA and at 4 ℃ unearthed 87d after sowing; and untreated cotyledons year unearthed.
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