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RUNX3 Regulates CD36 Expression in Melanoma Cell Lines

Author: WangLingHui
Tutor: LiuWenGuang
School: Northeast Normal University
Course: Cell Biology
Keywords: RUNX3 melanoma inhibit cell migration CD36
CLC: R730.2
Type: Master's thesis
Year: 2011
Downloads: 13
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Abstract


Recent analyses have revealed that RUNX family members play important roles in both normal developmental processes and carcinogenesis. Of the three known RUNX family members, RUNX3 has been shown to be involved in neurogenesis of the dorsal root ganglia,T-cell differentiation and tumorigenesis. RUNX3 is a known tumor suppressor gene in gastric carcinogenesis, pulmonary carcinosis, pancreatic carcinoma et al, and RUNX3 is frequently inactivated by allele loss or gene silencing due to promoter hypermethylation in several carcinomas.The RUNX3 transcription factor is a downstream effector of the transforming growth factor-beta (TGF-β) signaling pathway, and has a critical role in the regulation of cell proliferation and cell death by apoptosis, and in angiogenesis, cell adhesion and invasion.Reports of altered RUNX3 expression in melanoma were less, but based on studies of other malignancies, we hypothesized that RUNX3 is a tumor suppressor gene in melanoma and may relate to melanoma progression as in other cancers. We found that there were no RUNX3 expression in melanoma cell line B16F10, then we transfect RUNX3 expression plasmid and vector only into the B16F10 cell line to research the role of RUNX3 in B16F10. Compared with B16F10 cells transfected with vector only, the RUNX3-transfected cells is changed in cell morphology and cell scratch test results suggest that cell migration speed of RUNX3-transfected cells is slower than vector only.The results suggest that RUNX3 gene should inhibit melanoma cell migration.RT-PCR analysis shows that RUNX3 overexpression had no affect on integrinβ1, integrinβ3 and CD47 which are related to cell migration, but RUNX3 gene overexpression in B16F10 cells line results in significantly diminished CD36 expression.CD36, a member of scavenger receptor B family, is a transmembrane glycoprotein receptor that is expressed in a variety of cells, and implicated in the binding of lipoproteins, phosphatidylserine, thrombospondin-1, and the uptake of long-chain fatty acids. CD36 expression is prominent on the surface of platelets, capillary endothelial cells, macrophages. CD36, which on the surface of microvascular endothelial cell binds through its ligand TSP1, result in endothelial cell apoptosis and tumor angiogenesis. Recent studies suggest that in melanoma cells CD36 expression may induce the sequestration of certain integrins into membrane microdomains and promote cell migration.In our experiment RUNX3 negatively regulates CD36 expression in B16F10 cell, the down-regulation of CD36 expression by RUNX3 implies that RUNX3 inhibit melanoma cell migration by regulates CD36 expression.

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CLC: > Medicine, health > Oncology > General issues > Tumor pathology, etiology
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