Dissertation > Excellent graduate degree dissertation topics show

SERS Detection of Adenine and Cytosine by Patterned Silver Structure Immersion Plated on Silicon Nanoporous Pillar Array

Author: GuoYan
Tutor: LiXinJian
School: Zhengzhou University
Course: Condensed Matter Physics
Keywords: surface-enhanced Raman scattering (SERS) active substrate Ag/Si-NPA adenine cytosine
CLC: O621.1
Type: Master's thesis
Year: 2011
Downloads: 44
Quote: 0
Read: Download Dissertation

Abstract


The Raman spectrum belongs to the molecular vibration spectrum, provides the unique "fingerprint" information of molecular, and may apply in the fields of biological interaction analysis, disease diagnosis, analytical chemistry and so on. (?)wever, the normal Raman signal is extremely weak which limits its applications (?)eriously. The Raman signals can be efficiently enhanced by Surface-enhanced Caman scattering (SERS), which has been widely used in many fields. In the application of SERS, the selection and preparation of the active substrate is very important. In this article, the SERS substrate Ag/Si-NPA is fabricated by immersion plating based on silicon nanoporous pillar array (Si-NPA) which has regular (?)cture. Based on this prepared substrate, the Raman spectroscopy of adenine and (?)osine were obtained and analysed.The structure character of the SERS substrate Ag/Si-NPA was observed and (?)eral substrates of Ag/Si-NPA with different immersion time were prepared. Based on these substrates, the SERS spectrum of adenine with the concentration of (?)4 M were obtained and analyzed, and we found the best deposition time of the (?)tive substrate was 3 min.The SERS spectrum was analyzed and it could be inferred that the adenine molecules were vertically adsorbed on the surface of the substrate Ag/Si-NPA though N7. The SERS spectrum of adenine were obtained in low concentration from 10-5 to 10-9 M and it was found that the adenine was detected with the limiting concentration of 10-9 M. Through analyzing the transformation of vibrating peaks, we found the intensity of the peak 733cm-1 which characteristic the ring breathe vibration, become weaker gradually with the decrease of the adenine concentration. When the concentration of adenine was 10-8 M, the peak 733 cm-1 disappeared. According to the SERS surface selection rule, the state of adenine molecule absorbed un the substrate Ag/Si-NPA was changed. With the decrease of the concentration, the adenine molecules become parallel to the surface of the substrate from the state of the verticality. Then we studied where the adenine molecule adsorbed on the substrate when the SERS signal is best, and the result indicated when the adenine absorbed on the top of the silicon column, the SERS signal is perfect.The cytosine SERS spectrum has been obtained on the SERS active substrate of Ag/Si-NPA and simply analyzed its vibration peaks. The result, which is in accord with the literature report, is the cytosine molecule absorbed vertically with N3 on the surface of the active substrate. The SERS spectrums of cytosine with different concentration also been obtained and analyzed, and the result is the state of cytosine molecule absorbed on the substrate Ag/Si-NPA did not changed with the decrease of cytosine concentration.

Related Dissertations

  1. Electroporation Assisted Surface-enhanced Raman Spectroscopy for Living Cells,R318.51
  2. Effect of Sciatic Injection Injury on Enzymohistochemistry of Skeletal Muscle in Rabbit’s Legs,R-332
  3. Bone Mesenchymal Stem Cells with Cytosine Deamimase Gene Transducted in Migrat to Glioma in Vivio,R739.4
  4. Different electrical properties of carbon nanotubes covalently modified FAD molecular dynamics simulation,TB383.1
  5. Overexpression ANT1 Gene in Vascular Smooth Muscle Cells Induced Apoptosis with Bax/Bcl-2 Expression Relations,R541.4
  6. Fluorescence Determination of DNA and Electrochemical Behaviors of Based,Q523
  7. Mutant D314A of Escherichia Coli Cytosine Deaminase Enhances Its Antitumor Effect Significantly,R735.35
  8. The Effects of Yishen Soup on the Expression of Bone Morphogenetic Protein-7 and α-smooth Muscle Action of Renal Interstitial Fibrosis Model Induced by Adenine in Rats,R285.5
  9. Metabonomics-Based on Evaluation the Efficacy and Mechanism of Action of Rhubarb in the Treatment of Chronic Renal Failure,R277.5
  10. Based on research and application of anodic alumina template,TB383.1
  11. Preparation and Properties of Polyaniline-Noble Metal Nanocomposites,TB383.1
  12. Theoretical Study of the Intramolecular Proton Transfer in the Tautomers of Cytosine Assisted by Water,O626.41
  13. Several environmental POPs Analysis of Physical and Chemical Properties,X502
  14. Autophagy Inhibitor 3-Methyladenine Improves Learning Ability and Its Underlying Mechanism in a Neonatal Rat Model of Recurrent Seizures,R742.1
  15. Study on the Application of Ordered Mesoporous Carbon Modified Electrodes in Electrochemical Biosensors,O657.1
  16. Ordered Mesoporous Carbon Composite Materials and eletrocatalysis,O657.1
  17. Studies on the Relationship between Tonsillitis and Class Switch Recombination to IgA in IgA Nephropathy,R692.3
  18. Advanced glycation end products and homocysteine ??in human umbilical vein endothelial cells of vascular adhesion molecule -1mRNA effect and its mechanism,R541.4
  19. Studies on the Hydration of Adenine in Terms of Ab Initio and ABEEM/MM Fluctuating Charge Molecular Force Field,O621.13
  20. High-performance Capillary Electrophoretic Method for the Quantification of Global DNA Methylation and Methotrexate Polyglutamates in Cells,R450
  21. Chicken and experimental method and isozyme analysis and detection Eimeria resistance,S858.31

CLC: > Mathematical sciences and chemical > Chemistry > Organic Chemistry > Organic Chemistry general issues > Theory of organic chemistry,physical organic chemistry
© 2012 www.DissertationTopic.Net  Mobile