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Tea varieties with high EGCG content \effect was studied. Fixing technology for fresh findings suggest that, EGCG content in descending order fixing means fixing 11.13% microwave, oven, crank up 9.58%, 9.27% ??Guochao crank, steam fixing 8.44 percent, microwave fixation than the steam fixing, Guochao crank up, crank up the oven increase of 2.69%, 1.68%, 1.55%, so the fixing means fixing the microwave is good. Polyphenols extraction, separation and purification technology research results show that the optimum extraction conditions of polyphenol extraction solvent 50% ethanol, extraction 4 times, extraction time 50min, extraction temperature 70 ℃, extraction volume ratio of 1:50. DM301 resin than the D101, H1020 resin has better separation and purification of polyphenols performance. DM301 resin separation and purification of tea polyphenols specific parameter conditions are extracts PH value between 2 and 3, adsorption and desorption flow 2mL/min, desorption solvent 80% acetone. According to the above extraction, purification, high EGCG tea, Tieguanyin Huangdan leaf as raw materials, to obtain three kinds of polyphenols, tea polyphenols, the average extraction rate of 22%, an average of more than 95% purity, high EGCG tea polyphenols extraction rates were compared Tie Guan Yin, Huang Dan was 4%, 2%, low content of tea polyphenols EGCG EGCG tea 47.07% higher, respectively, 31.55% Iron Goddess of Mercy, Huang Dan 26.08%. High tea tea polyphenols EGCG EGCG content than the Iron Goddess of Mercy, Huang Dan high, respectively, higher than 15.52%, 20.99%. Isolation and purification of the EGCG showed high EGCG Huangdan tea polyphenols and at a flow rate of 0.5mL/min, 60% ethanol as eluting solvent to Sephadex LH-20 Sephadex column purified EGCG, high tea EGCG Huang Dan EGCG and polyphenols extraction rate were 33.3% and 16.6%, purity of 96% to 98%. Polyphenols free radical scavenging ability and anti-aging research in animals have shown that high polyphenol EGCG than regular tea varieties of tea polyphenols have a stronger ability to scavenge free radicals, Huang Dan polyphenols than radical scavenging capacity High of 9.37%. In Drosophila, the average life expectancy, the high content of tea polyphenols EGCG once more extended than the yellow flies average life expectancy increased by 14.5% (males), 14.9% (females), the value of SOD in Drosophila and total antioxidant value, the high content of tea polyphenols EGCG value than the yellow SOD increased 32.9% (males), 12.1% (females), total antioxidant capacity is increased by 44.1% (males), 28.5% (female) . Different extraction solvent extraction of polyphenols to extend the life of fruit flies is also different, with ethanol as extraction solvent ratio in boiling water for the extraction solvent extraction of polyphenols on the average life span in Drosophila increased 6.51% (male ), 6.45% (female), the value of SOD in Drosophila and the total antioxidant value, the ethanol extraction of polyphenols than the boiling water extraction can enhance the value of Drosophila SOD and total antioxidant capacity, SOD value increased of 9.53% (male), 9.43% (female), total antioxidant capacity is increased by 31.9% (males), 18.4% (females).
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