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The Study of Fertility、Feature of Flower Pigments and Heredity Performance of F1 Progenies in Dorotheanthus Bellidiformis

Author: LiuYaZuo
Tutor: NiuLiXin
School: Northwest University of Science and Technology
Course: Horticultural Plant Germplasm Resources
Keywords: Livingston daisies Self-incompatibility and Anthocyanidin F1 hybrids Genetic performance
CLC: S681.9
Type: Master's thesis
Year: 2011
Downloads: 14
Quote: 0
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Abstract


This study to Livingston daisies eight color strains of experimental material, Yangling Northwest Agriculture and Forestry University of Science and Technology, gardening test venue two years of cultivation and breeding trials, detailed records of their parents and ornamental characteristics, color reactions and UV - the visible spectrum scanning qualitative analysis the pigment species; incomplete diallel cross analysis of fertility and pollination, seed setting rate last observed hybrid F1 generation, measurement and recording, analysis of the traits of F1 hybrid inferred Gilman The genetic offspring Princeton daisies performance. The main results are as follows: artificial selfing and incomplete diallel cross Livingston daisies eight color strains to study, analyze the pollen viability, pollination methods, hybrid seed rate. The results show that: in vitro culture the Livingston pollen best medium is 250g / L sucrose 0.03g / L boric acid, and determination of The Livingston daisy pollen vitality accurate staining I2-KI staining eight strains Pollen viability of orange and yellow lines are strongest, followed by the the watermelon red lines and white lines, the pink yellow heart and the heart of the Rose Huang strains pollen viability of two strains greater than Pink the white Heart and Rose White Heart. Except white, orange and yellow three strains presented a very low self-compatibility, other strains tend to self-incompatibility. Seen by the rate of hybrid seed, orange, yellow and watermelon red stigmas of the three strains affinity is relatively poor, is not suitable as a female parent in the cross-breeding, but most pollen viability, the best choice of the male parent. Yellow two strains of pieces of red and white inside and Rose within the contrary, suitable hybridization paternal, not suitable for the female parent. Feature color reaction on UV - visible spectral scanning analysis, as well as at different temperatures, darkness and light conditions processing experimental results: Livingston daisies pigments belonging to the flavonoid compounds containing flavonoids and anthocyanins, may contain isoflavone, flavanone, dihydroflavonol without orange ketone and chalcone one; white lines only contain flavones and flavonols; Pink and white inside, orange and yellow three strains in addition to containing flavonoids and flavonols, may contain isoflavones; white gold Red Rose yellow and watermelon red pigment of the three strains contain anthocyanins, flavonoids, flavonols, and there is an acyl group; pink yellow and rosy red within yellow contains flavonoids, flavonoids the the alcohol Dihydroflavonol; eight color strains containing flavonoids containing an alcoholic hydroxyl and ketone hydroxy, does not contain phenolic hydroxyl; influence of temperature on the stability of the anthocyanin; dark treatment and light treatment anthocyanidin glycosides Stability There are significant differences between the strains of different color changes also has a significant difference. The conclusions show that the different color strains between the different types of flavonoids, temperature and light Livingston Daisy pigment stability, and the degree of impact of different color strains. Livingston daisies eight colors strains parent incomplete diallel cross, dubbed the 15 hybrid combinations, the observational record, and then analyze the genetic traits of F1 generation of flower color, flower diameter, polygamous, plant width and plant height performance. The results show that: Livingston daisy flower diameter of F1 generation plant width, and the height of its parent comparison showed a certain degree of heterosis, the hybrid total average flower diameter, plant width and plant height were equivalent to the value of 100.3 in pro %, 106.6% and 103.8%, respectively, but there are still individual portfolios recession phenomenon the comprehensive 14 combinations can be seen on the flower diameter, plant width and plant height, F1 hybrids showed significant maternal inheritance; suit genetic white invisible genetic gene belongs to the number of genetic heterozygosity, yellow, rose red and white inside, and Rose within yellow to white the dominant, orange, yellow and watermelon red, white Mahogany is a recessive genetic color genetic and cytoplasmic relationship.

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CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Annual and biennial flower class > Other
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