Dissertation > Excellent graduate degree dissertation topics show

Poly-L-Lysine-Dnalayer by Layer Modification on Medical Titanium Surface and Its Biological Properties

Author: GaoWenLi
Tutor: FengBo
School: Southwest Jiaotong University
Course: Materials Science and Engineering
Keywords: Titanium Layer self -assembly Polylysine DNA Biomineralization Protein adsorption Osteoblast culture
CLC: R318.08
Type: Master's thesis
Year: 2011
Downloads: 101
Quote: 0
Read: Download Dissertation

Abstract


Since titanium and titanium alloy having a low elastic modulus, high strength and toughness, good corrosion resistance and excellent abrasion biocompatibility, are widely as orthopedic biomaterial. However, titanium implants itself can not meet the requirements of the activity of biological material, so often for surface modification of materials to make a better biocompatibility. This study is divided into two parts, one on the titanium surface mount polylysine (PLL) and DNA, and self-assembly of the titanium surface modified bovine serum albumin (BSA) adsorption experiments, bio-mining of osteoblast cell culture experiments and experiments to evaluate the modified biological activity of titanium; second is the first with the anodic oxidation of the surface of the titanium oxide prepared in a diameter nanotube arrays, after heat treatment for the PLL and DNA The self-assembly, and then evaluate the surface of the protein adsorption capacity and biological mineralization, and finally through osteoblast cell culture experiments to study the biological activity of each sample. X-ray photoelectron spectroscopy, and the water contact angle data indicate that poly-lysine, and DNA could successfully self-assembled surface of titanium oxide and titanium oxide nanotube. From X-ray photoelectron spectroscopy data points P2p and N1s spectrum shows that polylysine and DNA COO-and NH3 through electrostatic attraction between, rather than physical adsorption combined together. Obtained by the quartz microbalance assembly of each layer of the amount of assembly, protons at 37 ℃ buffer, Ti surface of the PLL and DNA self-assembly layer has better stability. PLL using titanium nanoparticles and DNA self-assembly modified to measure the surface potential meter Zeta potential. Measurements show that the outermost surface of the PLL was positive, while the outer surface of the DNA was negative, and further verification of the PLL and the electrostatic interaction between the DNA. In the protein adsorption experiments, the UV-visible spectrum showed that the titanium oxide nano-tubes and the self-assembly of the protein to promote the adsorption layer, and the outermost surface of the PLL than the most outer layer of protein adsorbed on the surface of DNA more samples in each group were Ti / P / D / P and TNT / P / D / P BSA adsorbed on the surface up. In biomimetic experiment, scanning electron microscopy and X-ray diffraction results have shown that assembled on the surface of the PLL and DNA is more conducive to the deposition of hydroxyapatite. These results suggest that the presence of self-assembled monolayer of titanium and titanium oxide can improve nanotube surface protein adsorption ability and the ability of biomineralization. Osteoblast cell culture, the self-assembly process of cell surface modification spreading, proliferation and differentiation, each group were self-assembled sample Ti / P / D / P and TNT / P / D / P cells showed better compatibility, cell proliferation and differentiation capacity of the strongest. Moreover, the self-assembled layer of a smooth titanium surface compared to the surface of titanium oxide nanotube self-assembled layer is more conducive to cell adhesion, indicating that the presence of nanotubes affects the ability of cell adhesion and growth. These results show that the surface of titanium and titanium oxide nanotube layer self-assembly PLL and DNA to increase their surface activity, is an effective method for surface modification. When anodizing and interaction between the two layers of self-assembly, you can achieve the effect of co-promotion of cell adhesion, growth and differentiation promoting effect is more obvious.

Related Dissertations

  1. Studies on Synthesis, Characterization and Properties of Fe-V Co-doped TiO2 Catalyst,O614.411
  2. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii （Dunker） Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  3. Characterization and mechanism of the titanium dioxide surface envelope,TQ621.12
  4. Treatment Efficiency of Entecavir and TACE in Patients with Primary Heptatic Carcinoma of the HBV Copying,R735.7
  5. Establishment of the Molecular Identifying System of Curvualria and Application in Difficult Species,Q949.32
  6. Detection of Exogenous Recombinan DNA of Transgenic Rice and Its Rice Product,S511
  7. Primary Studies on Transcriptome and Population Genetics of An Invasive Weed Mikania Micrantha,S451
  8. Investigation of Cereal Cyst Nematode on Wheat in Jiangsu Province and the Sequence Analysis of rDNA-Its Region from Jiangsu Populations,S435.121
  9. Development and Identification of Structural Variations Involving Chromosome 1J and 5J of Th. Bessarabicum L(?)ve,S512.1
  10. Studies on Extracting Mitochondrial DNA and Gene Expressions in Brassica Napus L,S565.4
  11. Development and Identification of a Triticum Aestivum-Thinopyrum Bessarabicum Chromosome Translocation Line and Analysis on Its Genetic Effect,S512.1
  12. Studies on the Mechanism and Recovery Measures of Hyperhydricity of Pyrus Calleryana Decne in Vitro,S661.2
  13. In Vitro Rapid Propagation System of Peach Yu Dwarfing Rootstock No.1 and Optimization of Its SSR-PCR System,S662.1
  14. Effects of 5-Aza-2-deoxycytidine on DNA Methylation and Expression of p16 and MGMT Gene in Lung Cancer Cell Line SPC-A-1,R734.2
  15. Methylation of p16 Gene in Plasma and Tissues from Non-small Cell Lung Cancer Patients, Demethylation and the Biological Behavior of Lung Cancer Cell and Transcription of p16 Gene,R734.2
  16. DNA Prime/Protein Boost of Japanse Edcephalitis Virus NS1 Protein to Improve Immune Response in Mice,R392
  17. Analysis of DNA Barcoding on Partial Indigenous Duck Breeds,S834
  18. Molecular Phylogeny of Rhus Gall Aphids (Hemiptera: Aphididae: Pemphiginae) Based on DNA Sequences,S899.4
  19. The Preliminary Study of the pcDNA3.1(+)-SjP14 Nanometer Microballoon-DNA Vaccines,R392.1
  20. Effect of SO2 Exposure on DNA Methylation Polymorphism in Arabiadopsis Thaliana,Q943
  21. Iron, lanthanum -doped nano TiO \u003csub\u003e 2 \u003c/ sub\u003e Preparation and photocatalytic properties,O614.411

CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > General issues > Biomaterial
© 2012 www.DissertationTopic.Net  Mobile