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Studies of the Interaction of Several Antimicrobial Drugs with Bovine Serum Albumin Using Spectroscopy

Author: LiuQiuHong
Tutor: NiYongNian
School: Nanchang University
Course: Analytical Chemistry
Keywords: Bovine serum albumin (BSA) Small drug molecules Interaction UV - visible spectrum Fluorescence spectroscopy Chemometrics Conformational
CLC: R96
Type: Master's thesis
Year: 2011
Downloads: 113
Quote: 1
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Abstract


Bovine serum albumin studied at the molecular level I (Bovine serum albumin, BSA) small molecule interactions with drugs is the current hot topic in clinical medicine, life sciences and chemical field. In recent decades, a large number of scholars at home and abroad to the reports, but most of them are to study single drug with BSA. In this thesis, spectroscopy study of several antimicrobial drugs with BSA and chemometric methods applied to the spectral data analysis of complex biochemical system, resulting in the equilibrium concentration of each component and the pure spectrum, through which analytical spectrum can visually monitor the concentration of each chemical component in the whole course of the reaction trend can be calculated binding constant interaction of the drug with BSA. The more important is the chemometrics can resolve the spectrum peak of the new substances generated during the reaction, which is a conventional method of ships can not be obtained. Certain guiding significance of this research results on the combination therapy. The thesis includes the following five sections. Part I: the structure and function of serum albumin are briefly introduced, and the drug molecules and serum albumin (Serum albumin, SA) interaction research, research methods, and chemical metrology in drugs and SA interaction The study launched a more detailed review, and finally gave a brief overview of antibacterial drugs with the SA interaction Research. Part II: by fluorescence spectroscopy Ciprofloxacin (Ciprofloxacin CPFX) and gatifloxacin (Gatifloxacin, GTFX) stand-alone and co-exist with BSA, different two drugs with BSA interactions. The results showed that ciprofloxacin and gatifloxacin static fluorescence quenching with BSA and BSA binding quenching constant were calculated and found that gatifloxacin and BSA quenching constant greater than ciprofloxacin star quenching constant, two gatifloxacin mixing drugs with BSA quenching constant but also more than a single drug and BSA quenching constant. Probe competitive binding experiments show that two gatifloxacin are site I site of action in the BSA. Also by UV - visible and Fluorescence Spectroscopy the ciprofloxacin and BSA and gatifloxacin interaction, and chemometrics multivariate curve resolution - alternating least squares method (MCR-ALS) to resolve the resulting spectral data, the results show that the competitive effects between ciprofloxacin and gatifloxacin did not happen, but synergies and the formation of a ternary complex BSA-the concentration of CPFX-GTFX. Part III: Fluorescence Spectroscopy of Gold (Gold nanoparticles, GNPs) Ciprofloxacin (Ciprofloxacin CPFX), with BSA. The experimental results Mingjinnami and between BSA and ciprofloxacin combined effect. Gold is present, BSA and ciprofloxacin role of binding constants reduce the presence of ciprofloxacin, BSA and gold effect also reduces the binding constant, say between Mingjinnami and ciprofloxacin likely to compete. In addition, we also studied the the Gold with BSA ciprofloxacin inclusion, and chemometric methods parallel factor (PARAFAC) algorithm to resolve the three-dimensional data, found that the presence of gold hindered ciprofloxacin BSA binding may lead to easier release of ciprofloxacin, the efficacy of play faster. Part IV: by fluorescence spectroscopy to study two antibiotics Ciprofloxacin (Ciprofloxacin CPFX), and cefradine to be (cefradine. CF) alone while with BSA. The results show that, The ciprofloxacin and cefradine given with BSA process are static quenching the site of action of the drug on the BSA are site I; the coexistence of the two antibiotics, the presence of ciprofloxacin (cefradine) will make cephradine (ciprofloxacin) and BSA binding constants increase, combined with increased stability, decrease in free drug concentration, reduced efficacy; of ciprofloxacin and BSA cefradine role fluorescence spectrum also shows that ciprofloxacin Star cefradine between the presence of a combination of role, they formed a joint action with the BSA ternary complex BSA-the concentration of CPFX-CF; three-dimensional fluorescence spectroscopy experiments show that the conformation of BSA changes the order of addition of the two antibiotics. Part V: the interaction between the fluorescence spectroscopy and UV - visible absorption spectrometry chlortetracycline (the Chlortetracycline CTC) and oxytetracycline (Oxytetracycline, OTC) with bovine serum albumin (BSA). The results showed that chlortetracycline and oxytetracycline BSA static fluorescence quenching, and calculate the quenching constant and binding constants. Based on the energy transfer theory to determine the binding distance. According to the thermodynamic parameters and chlortetracycline with BSA force between the type of electrostatic attraction. And five metal ions on interaction, the article also found by synchronous fluorescence spectrometry and chlortetracycline have a certain influence on the conformation of BSA.

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