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The Th17/CD4~+FOXP3~+Treg Cells Imbalancce Expression in the Pathogenesis of Asthmatic Children

Author: MaQiuLi
Tutor: PengShao
School: Zhengzhou University
Course: Child medical
Keywords: Asthma Child Th17 cells The CD4 Foxp3 regulating T cells Flow cytometry IL-17 IL-10
CLC: R725.6
Type: Master's thesis
Year: 2011
Downloads: 82
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Abstract


Objective To detect Th17 cells in the peripheral blood of children with asthma, CD4 FOXP3 Treg cells and their related cytokines IL-17, IL-10 the expression levels, analyze and explore Thl7/CD4 FOXP3 Treg cells involved in childhood asthma in the pathogenesis of children the prevention and treatment of asthma provide a theoretical basis. Method 1. Asthmatic children (n = 103) as the main object, according to the condition of children with asthma were divided into two groups: acute exacerbation group (n = 71), remission group (n = 32); acute asthma The children were divided into 3 groups according to the severity of the disease: mild group (n = 30), moderate group (n = 26), severe group (n = 15). Healthy children (n = 30) for the control group. 2 separate groups PBMC intracellular staining was measured by flow cytometry (FCM) Th17 cells in the peripheral blood, the percentage of CD4 of FOXP3 Treg cells. 3 by enzyme-linked immunosorbent assay (ELISA) method for the determination of IL-17, IL-10 expression level in the peripheral blood plasma. Results 1.Th17 percentage of cells, IL-17 expression level: acute exacerbation group were higher than the remission group and control group, higher than the control group in remission (P lt; 0.05); acute exacerbation of severe group than in the moderate group and mild group were significantly increased (P lt; 0.05) 2.CD4 FOXP3 Treg cell percentage, the level of IL-10 expression: acute exacerbation group than in the remission group and the control group was significantly lower (P lt; 0.05); acute exacerbation of severe group was significantly lower than the moderate group and mild group (P lt; 0.05); reduce moderate group and mild group (P lt; 0.05). 3.Th17 cell percentage of peripheral blood plasma IL-17 expression levels were positively correlated (r = 0.817,19 lt; 0.0001); childhood asthma severity and Th17 cell percentage and peripheral blood plasma of IL-17 expression levels were positively correlated (r = 0.649,0.873, P lt; 0.05). 4. CD4 FOXP3 Treg in the percentage of cells with peripheral blood plasma IL-10 expression level was being related (r 0.235 P lt; 0.05);, CD4 of FOXP3 Treg cell percentage and IL-10 expression levels and children with asthma situation serious extent was negative Relevance (r = -0.599, -0.241, P lt; 0.05). Asthma in PBMC of Th17 and Treg cell percentage was negatively correlated (r = -0.672, P lt; 0.05) Conclusion 1.Th17 cells and their secretion of IL-17 as a pro-inflammatory factors involved in the pathogenesis of asthma in children, and increased with the aggravation of the disease of asthma in children; CD4 FOXP3 Treg cells and their related cytokines IL-10 involved children the incidence of asthma, severe asthma in children may be present CD4 FOXP3 Treg cell functional defects and IL-10 synthesis disorders. Glucocorticoid (budesonide) corrected Th17 cells / CD4 FOXP3 Treg cell imbalance, systemic inflammatory response in children with asthma play an inhibitory; IL-10 expression level of slow recovery. 3 Th17/IL-17 axis as a pro-inflammatory factors involved in the pathophysiology of asthma. CD4 FOXP3 Treg cells / IL-10 involved in the pathogenesis of asthma in children, and to play an inhibitory effect in the course of the inflammatory response, IL-17 and IL-10 is expected to become a new indicator of the diagnosis and treatment of childhood asthma, childhood asthma immunotherapy new targets. 4.Th17/Treg cell immune imbalance is closely related to airway inflammation of asthma in children, to make up for the lack of Th1/Th2 cellular immune imbalance in the pathogenesis of asthma in children, further supplement and improve children pathogenesis of asthma, for the treatment of asthma opened up a new way of thinking.

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CLC: > Medicine, health > Pediatrics > Children within the science > Department of pediatric respiratory and chest diseases
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