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Finishing summarize the main purpose of ancient physicians on hemorrhagic stroke pathogenesis exposition to explain Didangtang basis for treating hemorrhagic stroke, demonstration Didangtang treat hemorrhagic stroke reasonable and scientific. And in previous studies of brain hemorrhage model based on the combination of acute hemorrhagic stroke characteristics of the pathogenesis, development of hypertensive cerebral heat syndrome of blood stasis model from ethology, syndrome related indicators and micro indicators point to evaluate the model ; and observe Didangtang of the model, and to explore Didangtang acute hypertensive cerebral blood stasis and heat of the possible mechanisms for the capture of stasis treatment of hypertensive cerebral heat syndrome of blood stasis provide experimental basis. Methods This study is divided into two parts, theoretical and experimental studies. Theoretical part: a review of our ancient literature, reference discusses today's physicians and published journals, collate summary ancient physicians on hemorrhagic stroke prevention point of view, and describes the capture of stasis treatment of hemorrhagic stroke basis. Experimental part: using the modified method of two kidney one clip renal hypertensive rat model. In hypertensive model successfully copied after intraperitoneal injection of endotoxin (LPS), stereotaxic injection of collagenase, copy cerebral hemorrhage rat model. Then were applied Didangtang its decomposition peach rhubarb, leeches horsefly insect treatment one week. Rats were observed Normally, blood pressure, neurological function score (Bederson, LongaEZ, equilibrium experiments), pathological changes in serum levels of superoxide dismutase (SOD), malondialdehyde (MDA), cerebral hematoma surrounding tissue Na - K-ATP enzyme, interleukin -8 (IL-8), protein C, nerve cell adhesion molecule -1 (ICAM-1), prothrombinase -2 (FGL-2), heme oxygenase - 1 (HO-1), angiopoietin -1 (Ang-1) levels. Using SPSS17.0 statistical package for data statistical methods, data to x ± s, said measurement data using single factor analysis of variance between the two groups were compared using t-test comparison between groups using analysis of variance F test. Homogeneity of variance is used LSD-t method, heterogeneity of variance using Dunnett-t method. Significance level α of 0.05 (homogeneity of variance test α of 0.10). P lt; 0.05 was considered statistically significant. Theoretical study results: The efficacy of having scored stasis Didangtang treatment of hemorrhagic stroke is feasible and science, has its use basis. Experimental Study: model and evaluation: In general: The model rats appear to reduce dietary substance, do not like moving, conjunctival hyperemia, ataxia, limb weakness or paralysis, even death and other symptoms; sham-operated rats spirit, activity, diet, no significant change. Blood pressure: model rats increased blood pressure, compared with the sham group, a significant difference (P lt; 0.05). Neurological score Id: Bederson Rating: model group were rated 2.22 points or so, sham score of 0 points, compared the two groups, a significant difference (P lt; 0.05). LongaEZ Rating: model group score 1.72 points or so, sham score 0 points, compared the two groups, a significant difference (P lt; 0.05). Balancing test: the model group score 4.28 points or so, sham score 1.71 points or so, two groups, a significant difference (P lt; 0.05). Pathological observation: visible light microscope, model group, a large number of red blood cells leak bleeding area, staining shallow, brain tissue shows red blood cells, inflammatory cells and brain cell gap becomes large, loose tissue around the hematoma, softening, degeneration and necrosis or even disappear, dyeing lighter, more red blood cells, leukocyte infiltration, and visible brain cell gap becomes larger, cell swelling, hematoma seen around more hemosiderin deposition; sham tissue space more closely, erythrocyte distribution, no significant inflammatory cell infiltration. Biochemical testing: the model group decreased SOD activity and MDA content increased, Na-K-ATP enzyme activity was reduced, compared with the sham group, were statistically significant (P lt; 0.05). Immunohistochemistry: IL-8, ICAM-1, FGL-2, model group appear more positive cells sham group only see a small amount of positive cells compared two groups, a significant difference (both P lt; 0.05); protein C, the model group were less positive cells appeared in sham group more positive cells compared two groups, a significant difference (both P lt; 0.05). Western blot immunoblot assay: HO-1, Ang-1 expression in the model group was decreased, compared with the sham group, a significant difference (P lt; 0.05). Didangtang its decomposition treatment: blood pressure in the treatment groups, compared with the model group, no significant difference (p gt; 0.05). Neurological score 7d: Bederson Rating: All parties rats score 1.54 points or so, rhubarb peach group at around 1.42, compared with model group, a significant difference (P lt; 0.05). LongaEZ Rating: rhubarb, peach group score 1.08 points or so, compared with model group, a significant difference (P lt; 0.05). Balance experiment: rhubarb peach group scores 3.67 points or so, compared with model group, a significant difference (p lt; 0.05). Pathological observation: omnidirectional brain tissue cell gap becomes larger, the bleed area hemosiderin deposition, red blood cells, leukocyte infiltration; rhubarb peach bleed area larger group of hemosiderin deposition, red blood cells, leukocyte infiltration, compared with brain tissue cell gap All parties larger group; leeches bleed area horsefly insect groups scattered hemosiderin deposition, fewer red blood cells, leukocyte infiltration, brain tissue cell gap becomes larger, but smaller than the whole party group. Biochemical testing: SOD activity in each treatment group, Na-K-ATP enzyme levels increased and MDA content decreased, compared with model group, the treatment groups were statistically significant (P lt; 0.05). Immunohistochemistry: IL-8, positive cells in each treatment group decreased compared with model group, a significant difference (P lt; 0.05). ICAM-1, decreased expression of all party group, leeches horsefly insect groups increased expression, two Compared with model group, a significant difference (P lt; 0.05). Rhubarb peach group, compared with model group, no significant difference (P gt; 0.05). Protein C, positive cells in each treatment group increased compared with model group, a significant difference (P lt; 0.05). FGL-2, positive cells in each treatment group decreased compared with model group, a significant difference (P lt; 0.05). Western blot immunoblot assay: HO-1, increased expression of all party group, compared with model group, a significant difference (P lt; 0.05). Rhubarb peach group, leeches horsefly insect compared with the model group, no significant difference (P gt; 0.05). Ang-1 expression, the whole side group, rhubarb peach group was increased compared with model group, a significant difference (P lt; 0.05), leeches horsefly insect compared with the model group, no significant difference (P gt ; 0.05). Conclusion Didangtang treat hemorrhagic stroke, theoretical, clinical, experimental basis, can be used to treat hemorrhagic stroke. Renal artery stenosis ligation experiments using intraperitoneal injection of endotoxin LPS, intracerebral injection of collagenase hypertensive rat brain multifactorial heat syndrome of blood stasis model, which is the formation of cerebral hemorrhage and clinical development process close to histopathology observation, neurological assessment, biochemical and immunohistochemical index test results show that the model can simulate some characteristics of hypertensive cerebral hemorrhage using stereotaxic techniques, endotoxin, collagenase intracerebral injection method can be successfully produced multi-factor complex in rats the heat of hypertensive cerebral blood stasis model. Experimental results show that Didangtang its decomposition on hypertensive cerebral blood stasis heat of the therapeutic effect may be through increased SOD content, Na-K-ATP enzyme activity, decreased MDA content, reduce the redox reaction of free radicals on the body injury, improve brain tissue for energy; inhibited FGL-2 expression, and promote expression of protein C, to improve blood coagulation and anticoagulation system disorders; reduced IL-8, ICAM-1 and other inflammatory mediators, release, reduce or interrupt inflammation chain reaction; promote HO-1, Ang-1 expression, cutting ferrous heme-mediated oxidative stress, promote angiogenesis, to reduce apoptosis, neuronal injury, cerebral edema, protect nerve cells, to treat brain Bleeding purposes. The results suggest that arrived when the decoction and its treatment of cerebral hemorrhage is the role of targets through multi-channel multi-role, and its possible mechanism by inhibiting the inflammatory response, antioxidant free radical damage, improve brain tissue coagulation and anticoagulation system disorders, promote angiogenesis and therapeutic purposes hemorrhagic stroke, however, its specific mechanism of action need to be further discussed.
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