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Small flowers Yumei (Anemone rivularis var. Flore-minore Maxim.) Belongs to Ranunculaceae (Ranunculaceae) Anemone (Anemone), is a perennial herb. Alias: mountain pepper (Shaanxi), of Pulsatilla (Gansu), breaking bidentata (Ningxia), grown in 900 ~ 3000 m above sea level hillside wetlands, mountain forest edges or grassy bank, located in the north, northwest, and Liaoning, Henan, Sichuan other provinces, the Qinling Mountains North Slope have a large number of distribution sources readily available. Folk its the whole plant or rhizome medicine, \Currently, research is focused on small flowers Yumei in terms of morphology, chemical composition and pharmacological research has not been reported. Through the literature search, the same plant containing triterpenoid saponins. The small flowers Yumei crude extracts of anti-tumor activity screening (BEL-7402, HeLa), found certain tumor cell toxicity (LD50 were 176.3μg/ml, and 183.2μg/ml). The paper mainly around the chemical composition of small flowers Yumei Expand studies, using a silica gel column chromatography, a variety of means of separation and purification of the open ODS column chromatography, Sephadex LH-20 gel column chromatography, preparative high performance liquid chromatography, etc. small flowers Yumei whole grass of water saturated n-butanol layer extract isolated Compound 16, the use of ESI-MS and 1 to H-NMR (13) to the C-NMR, DEPT135, HSQC, HMBC, for 1 ~~ H-1 to H COSY, NOESY, TOCSY, and other methods and combined with chemical means of identification of the structure of the compound 15, respectively, for :3-O--L-arabinopyranosyl - oleanolic acid-28-O--L-pyran rat Li sugar - (1 → 4)-β-D-glucopyranosyl - (1 → 6)-β-D-glucopyranoside glycoside (1); 3-O--L-arabinopyranosyl - Hederagenin Element-28-O--L-rhamnopyranosyl - (1 → 4)-β-D-glucopyranosyl - (1 → 6)-β-D-glucopyranoside 3-O (2); -β-D-glucopyranosyl - (1 → 2) - L-arabinopyranosyl - ivy sapogenin-28-O--L-gluco-rhamnose - (1 → 4)-β-D - glucopyranosyl - (1 → 6), β-D-glucopyranoside glycoside (3); 3-O-β-D-pyran-ribose - (1 → 3) - L-rhamnopyranosyl - (1 → 2) - L-arabinopyranosyl - Hederagenin-28-O--L-gluco-rhamnose - (1 → 4)-β-D-glucopyranosyl - (1 → 6 ) glycoside (4); the β-D-glucopyranoside 3-O--L-arabinopyranosyl - Hederagenin (6); ivy sapogenin-28-O--L-pyran-rhamnose sugar - (1 → 4)-β-D-glucopyranosyl - (1 → 6)-β-D-glucopyranoside (7); 3-O--L-arabinopyranosyl - ivy sapogenin -28-O-β-D-glucopyranoside (8); 3-O-β-D-glucopyranosyl - (1 → 2) - L-pyran-arabinose - oleanolic acid -28 - 3-O--L-O--L-rhamnopyranosyl - (1 → 4)-β-D-glucopyranosyl - (1 → 6)-β-D-glucopyranoside (9); arabinopyranosyl - ivy sapogenin-28-O-β-D-glucopyranosyl - (1 → 6)-β-D-glucopyranoside (10); 3-O--L-pyran-Arab Sugar - silk carnation sapogenin-28-O--L-gluco-rhamnose - (1 → 4)-β-D-glucopyranosyl - (1 → 6)-β-D-glucopyranoside (11 ); ivy sapogenin-28-O-β-D-pyran-glucoside (12); 3-O-β-D-glucopyranosyl - (1 → 2) - L-arabinopyranosyl - Homogeneous oleanolic acid (13); 13 (S) - hydroxy-7 - O - the Helianthemum alkoxy-8 ,14 - diene -19 - acid-β-D-galactose ester glycosides (14); (7S, 8R, 7'R, 8'S) 4,9,4 ', 7'-tetrahydroxy -3,3' - dimethoxy-7'-O-n-butyl -7,9 '- tetrahydrofuran 9-O-β- D-glucoside (15); Hederagenin (16). Among them, Compound 14, 15, 11 is a new compound, the compound 11, 12, 14, 15 for the first time from Anemone genus, all compounds were first small flowers Yumei obtained. The above work to fill the blank of the of small flowers Yumei chemical ingredients, rich in the chemical composition of the genus Anemone, reference data for the the small flowers Yumei chemical classification. This main component separation plants Anemone characteristic ingredients (oleanane type triterpenoid saponins) coincide small flowers Yumei vested in Anemone confirmed, for the first time from the perspective of chemical ingredients. Same time, the literature search, the diterpene glycosides compounds (14) and lignan glycosides (15) previously reported no separation Anemone, reflecting the small flowers Yumei as a variant (Anemone plant grass Yumei variants) particularity. BEL-7402 and HeLa cell toxicity test results show, part of monomer compounds compounds 6, 13 have significant anti-tumor activity, combined with the experimental results of preliminary structure-activity relationship speculated for future pharmaceutical development reference data.
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