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Effects of Qindan Capsule on Expression of Vascular Adventitial Ⅰ Collagen and Ⅲ Collagen on Old Spontaneous Hypertensive Rats

Author: GuoXueFeng
Tutor: ZhangJiDong
School: Shandong University
Course: Traditional Chinese Medicine
Keywords: Qindan Capsule Hypertension Spontaneously hypertensive rats Adventitia reconstructed Ⅰ, Ⅲ collagen
CLC: R259
Type: Master's thesis
Year: 2011
Downloads: 13
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Abstract

Purpose. Observed Qindan capsule on the blood pressure of spontaneously hypertensive rats. 2. Observed Qindan Capsule on vascular adventitia Ⅰ, Ⅲ collagen expression, and to explore Qindan capsule reversal of the hypertensive vascular adventitia reconstruction mechanism. Method 1. Study: 32 40-week-old male SHR rats were randomly divided into four groups, the model group (SHR group), high-dose group Qindan Capsule the (SHR QDH group), Qindan capsule low dose group (SHR a QDL group ), losartan group (SHR Los group), another 40-week-old male WKY rats 16 divided into blank control group (WKY blank group) and normal the drugs control group (WKY QD group). The the SHR QDH group to give Qindan capsule 750mg / (kg · d); the SHR a QDL groups to give Qindan capsules 150mg / (kg · d); SHR Los group were given losartan 30mg / (kg · d); in WKY group was given QD baicalensis Capsule 750mg / (kg · d); in WKY control group and SHR group was given equal doses of saline, 1 times / d, continuous administration of 12 weeks. Rats in each group after the last dose, fasting for 24 h, and can not help but water. 10% chloral hydrate anesthetized by intraperitoneal injection for subsequent experiments. Study: (1) rat tail artery systolic pressure was measured. (2) by immunohistochemical method observation the thoracic aortic adventitia I III collagen expression. (3) real-time quantitative PCR detection of thoracic aortic adventitia Ⅰ, Ⅲ collagen expression. (4) using western blotting was detected Ⅰ collagen expression. Results rats before and after treatment, systolic blood pressure compared in Figure 1, starting from the first four weeks of the administration, SHR QDH group, SHR QDL group, SHR Los groups, respectively, compared with the SHR group, blood pressure decreased significantly, their differences were statistically significance (P lt; 0.05). Administered 6 weeks to 12 weeks, SHR QDH group decreased more significantly than the blood pressure in SHR QDL group, a statistically significant difference (P lt; 0.05). Thoracic aortic tissue immunohistochemistry measurement results shown in Table 2, Figure 2 and 3, the the SHR group of WKY blank group Ⅰ, Ⅲ collagen expression increased significantly, and the difference was statistically significant (P lt; 0.05); the SHR QDH group, the SHR a QDL group and SHR Los group expression compared with SHR group were reduced (P lt; 0.05). WKY control group and WKY QD group Ⅰ, Ⅲ collagen expression levels compared to the difference had no statistical significance (P gt; 0.05), the SHR QDH group SHR ??QDL group, the difference was statistically significant (P lt; 0.05), but with SHR Los group, the difference was not statistically significance (P gt; 0.05). 3. Aortic wall Ⅰ, Ⅲ collagen mRNA expression levels compared using the 2-ΔΔCt method, type Ⅰ, Ⅲ collagen gene expression of the target gene Ct values ??converted to changes multiples relative to the the WKY blank group, can be obtained directly phase reference gene quantitated. Shown in Figures 6 and 7 respectively Ⅰ Ⅲ collagen amplification, dissolution profile and the dissolution peak described Ⅰ Ⅲ collagen is a specific gene expression, no primer dimer and non-specific amplification. The results show that: Figure 4, the difference in WKY blank group and WKY QD group was not statistically significant (P gt; 0.05), the SHR QDH group and SHR QDL group difference was statistically significant (P lt; 0.05). The SHR rats thoracic aortic wall Ⅰ collagen type III mRNA expression between WKY group, the difference was statistically significant (P lt; 0.05). The the the SHR QDH group, the the SHR a QDL group and SHR Los Groups rat thoracic aortic wall Ⅰ expression of collagen type III and WKY rats increased significantly compared to decrease compared with the SHR group, the difference was statistically significant (P lt; 0.05). 4. Ⅰ collagen protein in immunoblot expression group of WKY blank, in WKY QD group, SHR Los group, SHR QDH group, SHR QDL group, SHR group protein expression were enhanced collagen type I molecular size around 120KD reference to actin The molecular weight of 43KD, the protein sequentially increasing trend can be seen. Shown in Figure 5, to do a statistical analysis of the results of the collagen type I and actin ratio: differences in WKY blank group and WKY QD group was not statistically significant (P gt; 0.05), the SHR QDH group and SHR QDL group difference was statistically significance (P lt; 0.05). SHR model group and normal WKY, the difference was statistically significant (P lt; 0.05); SHR QDH group. SHR QDL group SHR ??Los group compared with the SHR group, the difference had statistical significance (P lt; 0.05) ; the SHR QDH groups SHR Los group difference was not statistically significant (P gt; 0.05). Conclusion Qindan Capsule significant antihypertensive efficacy of SHR rats reduce its buck mechanisms Qindan capsule in SHR Ⅰ, Ⅲ collagen expression levels related with both reversing vascular adventitia remodeling, inhibition of adventitial fibrosis, reduce Ⅰ, Ⅲ Synthesis and deposition of collagen in the adventitia.

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