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Role of Cell Adhesion Molecules and Gap Junctional Intercellular Communication in the Invasion and Metastasis of Lung Cancer and Study of Drugs Regulation

Author: ChenQingYong
Tutor: ZhengZuoXiang；ZhouJianYing
School: Zhejiang University
Course: Biomedical Engineering
Keywords: Tea polyphenols Compound Danshen Lung cancer cells Endothelial cells Tumor metastasis CD44 protein CD54 protein E - cadherin Gap junctional intercellular communication Gap junction protein Intracellular calcium ion concentration Cell cycle Confocal laser scanning microscope Flow cytometry Immunohistochemistry
CLC: R734.2
Type: PhD thesis
Year: 2002
Downloads: 344
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Abstract

Cancer patients encountered in clinical work mostly has been transferred at the time of diagnosis. This is the cancer treatment process is a very complex and important issues, although the primary tumor surgical resection or radiation therapy, chemotherapy and immunotherapy, metastases often poor effect of the above measures. Clinical observations indicate that most of the patients died of metastasis. Primary lung cancer is a common malignancy, the incidence is increasing, and has now become one of the highest cancer mortality tumor. The surgery is very important in the treatment of lung cancer, the possibility of postoperative adjuvant chemotherapy and radiotherapy can reduce local recurrence, but does not improve survival. Simple surgical treatment Ⅰ Ⅱ non-small cell lung cancer treatment options, but 50% of the clinical stage Ⅰ patients still die of recurrence, indicating that although the surgery is the best treatment of lung cancer, metastatic recurrence of tumor leading cause of death, whether the recurrence and metastasis of lung cancer patients after successful prevention is one of the keys to the treatment of lung cancer, is an important topic of current research. Cell adhesion molecule (CAM) is involved in cell - cell and cell - matrix between the role of a class of substances adhesion general term, including the family of cadherin and integrin family, the immunoglobulin superfamily and selectin family, transparent a hyaluronic acid receptor (CD44) and CAM. Recent studies show that plays an important role in the CAM in tumor invasion and metastasis, adhesion between tumor cells and endothelial cells is a critical step in metastasis of tumor blood inhibit the interaction between tumor explore transfer mechanism and to establish the role of the drug has important significance. GJIC (gap junction intercellular communication, GJIC) is an important connection between the cell and play an important role in cell differentiation, growth control and maintain the environmental balance in the body. GJIC homology and similarities and differences borne the past, some studies have shown that, GJIC associated with tumor growth and metastasis. Homology and similarities and differences in tumor growth, derived GJIC abnormality first is formed in the tumor and maintain the tumor phenotype. Some experiments show that the communication between tumor cells and endothelial cells by gap junction channel-mediated tumor cell metabolites transfer from the cytoplasm to the vascular endothelial cells exposed to the adhesion of tumor cells by inducing local endothelial cell retraction and subendothelial matrix The foreign invasion of tumor cells. To explore these mechanisms, we studied the in vitro and in vivo tumor cells metastatic potential relationship with GJIC and CAM. PG cells of lung cancer growth and metastasis Mechanism and regulation of drug research. The experimental method of intracellular Ca concentration, CD44 expression, gap junctional intercellular communication and its protein in lung cancer metastasis. MTT method was used to observe the killing effect of different concentrations of tea polyphenols on PG cells; application of laser scanning confocal microscopy and flow cytometry analysis, the PG cells detect medication Caz \2. The experimental results f polyphenols concentration of PG cells were killing effect was dose-dependent. Was significantly inhibited cell proliferation, cell cycle arrest in GO issues, can not enter S phase and GZ / M phase, the cell proliferation index decreased. Intracellular Caz \confocal microscopy and flow cytometry double fluorescent labeling, in vitro studies the different metastatic lung cancer cells and vascular endothelial cell adhesion and gap junctional intercellular communication and adhesion molecule expression levels and detection of polyphenols and Salvia above process. experimental results highly metastatic lung cancer cell furnace G cells) and vascular endothelial cell adhesion capacity, level of gap junctional intercellular communication and expression of adhesion molecules (CD44, CD54) was significantly higher than the low metastatic lung cancer cells ① cells) when the vascular endothelial cells the u (0.sug/ml) activation, PG cells to their adhesion and gap junctional communication function and adhesion molecule expression significantly increased compared activate. polyphenols and Salvia on PG cells and vascular endothelial cell adhesion, gap junctional intercellular communication significantly inhibited, and can inhibit the adhesion molecules of CD44, CD45 expression was dose-dependent. gap junction protein E-cadherin in lung cancer Expression and clinical significance of the organization. experimental method using immunohistochemistry the ABC method 5 (Sichuan 39 primary lung cancer CX43 and Bu cadherin prime expression, and analysis of their expression with clinicopathological relationship. 2 experimental results with tumor progression, CX43 and Bu calcium cadherin expression gradually reduce, CX43 and Bu cadherin positive expression rate of 1,11 of non-small cell lung cancer was significantly higher than *, Phase IV; well differentiated in lung its of CX43, E-cadherin positive expression rate was significantly higher than that in poorly differentiated lung cancer; lung cancer with lymph node metastasis Pu cadherin expression levels lower than those without lymph node metastasis: no significant correlation of CX43 expression and lung cancer lymph node metastasis . CX43 positive expression rate of lung adenocarcinoma was significantly higher than that of squamous cell carcinoma and large cell undifferentiated carcinoma (P <0.05), the expression of E-cadherin of non-small cell lung cancer tissue type was no significant correlation sex. confocal microscope quantitative detection gap junction protein E-cadherin-hormone expression in lung cancer 11. experimental methods in this?

Role of Cell Adhesion Molecules and Gap Junctional Intercellular Communication in the Invasion and Metastasis of Lung Cancer and Study of Drugs Regulation

Abstract

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