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The Study of the Mechanism of Spinal Cord Injury and the Effect of Adenovirus-mediated GDNF Transfer in Vivo on Recovery of Motor Function Following Moderate Spinal Cord Injury

Author: WuZuoTian
Tutor: ZhangYingZe;PanJinShe
School: Hebei Medical University
Course: Surgery
Keywords: Spinal cord injury Decompression Injection volume Glial cell line-derived neurotrophic factor Gene therapy
CLC: R651.2
Type: PhD thesis
Year: 2005
Downloads: 204
Quote: 0
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Abstract


The experimental spinal cord injury research has been the research focus of neurology and orthopedic. Spinal cord damage depends on injuries caused by violence in size and degree of spinal cord shift primary, bone, ligament shift and spinal hematoma is caused by continued pressure on the spinal cord, thus aggravating the spinal cord injury. The present study suggests that spinal cord damage caused by the secondary injury is far more than the damage caused by mechanical deformation, timely and effectively relieve spinal cord compression can reduce secondary injury, there is an important role for the protection of nerve function. Spinal cord compression time relationship with neurological impairment is not clear, when surgery still no consensus on the best period. With the deepening of basic scientific research, awareness to the effect of treatment depends on the protective effect on neurons and promote axonal regeneration. The administration of exogenous neurotrophic factors can protect neurons and promote axonal regeneration. However, due to exogenous neurotrophic factor relative molecular mass, not through the blood-brain barrier, and the short half-life have not been able to clinical application. The gene therapy SCI a new treatment, the transfected cells can continue to secrete the required nutritional factors, protect neurons and promote axonal regeneration and recovery of neurological function, can play a long-term effect of transgenic technology. In the currently known neurotrophic factor, GDNF is active the strongest movement neurotrophic factor. As gene transfection vectors, adenovirus vectors to facilitate the preparation of a wide host range, high transfection efficiency advantages, is currently considered the ideal carrier gene transfection. Following to the experiment, applied to a spinal cord injury, spinal cord parenchyma microinjection amount of liquid from 0.5ul to 6ul size ranging from spinal cord injury has no theoretical basis, will increase the size of the injection amount of liquid and injection. Based on this this topic by observing the pathological changes of the spinal cord injury, spinal cord gray matter blood flow, somatosensory evoked potentials and motor function changes, explore the organizational relationship between compression time and the degree of spinal cord injury and nerve function recovery. And to observe the size of the injection volume of spinal cord function by a different amount of liquid injection to the spinal cord parenchyma, injection volume explore the safety of gene therapy for spinal cord injury. Thus safe amount of adenovirus-mediated glial cell line-derived neurotrophic factor in vivo transfection treatment of moderate spinal cord injury, to observe the impact on the recovery of neurological function, and for the the clinical final application of recombinant adenovirus injury lay the foundation for the treatment of spinal cord injury. Spinal cord compression time correlation with the degree of injury

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CLC: > Medicine, health > Surgery > Of surgery > Head and Neurosurgery > Spinal cord
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