|
In recent years , fixed on a solid phase carrier nucleic acids ( oligonucleotides , cDNA , and PCR products, etc. ) molecules to prepare the DNA micro- set array (DNA microarray) is one of the key technology of the production of a gene chip or miniaturization of hybridization arrays , has become an important biological and medical research tool , and is widely used in the detection and polymorphism analysis of the gene mutation , disease surveillance and antisense drug screening , and gene expression changes in monitoring field . The immobilization of nucleic acid is the first problem to be solved in the preparation of gene chips . In this process needs to be carried on the carrier surface chemical treatment, thereby effectively to a variety of nucleic acid probes specific , fast and stably fixed on the modified surface . It directly affects the performance of the micro-array , and plays a crucial role in the chip preparation . Therefore , research and development in this field , will help promote China 's large - scale gene chip independent production and application . Fixed nucleic acid technology is related to surface chemistry, molecular biology, interdisciplinary , including physical adsorption and chemical coupling ( covalently linked ) into two categories . More traditional fixed mainly physical adsorption , surfactant connect low efficiency , poor stability defect . The covalently linked to the introduction of the surface treatment of glass slides and nucleic acids fixed : improved classic poly - L - lysine (poly-L-lysine) coated Act ; own research of two based on the multi-component the connection system of the amino compound and acrylic acid - acrylamide polymer-based
|