Microbial enhanced oil recovery (MEOR) is a technique to utilze microorganisms and their metabolic products to improve the recovery of crude oil from reservoir rocks. MEOR " />
Dissertation > Excellent graduate degree dissertation topics show

Oil filters from the city flooding the soil physicochemical properties and identification of bacteria

Author: ChenZuoZuo
Tutor: LiShengXian
School: Heilongjiang University
Course: Microbiology
Keywords: Microbial enhanced oil recovery oil bacteria metabolic products surface active agent
CLC: TE357.9
Type: Master's thesis
Year: 2011
Downloads: 43
Quote: 0
Read: Download Dissertation

Abstract


oil recovery' dissertation">Microbial enhanced oil recovery (MEOR) is a technique to utilze microorganisms and their metabolic products to improve the recovery of crude oil from reservoir rocks. MEOR was the technology that poured microorganism and microbial metabolic products into crude oil in order to improve crude oil recovery ratio by some microbial actions or some characters of microbial metabolic products acted to crude oil and then using these actions or characters could change physical and chemical specialities of the crude oil.Bacteria are the only microorganisms which have been proposed for enhanced oil recovery process. They are small in size, grow exponentially and tolerate dicky environments such as : high temperature ,high water salinity and high pressure.They can enhance oil recovery by producing metabolic compounds such as gases,acids, surfactants and polymers. The strains which could produce acid, gas or surfactant were sifted from extended pollutional water and soil,and their physical ,chemical properties and 16S rDNA sequence were analyzed synchronously in our test in order to identify these bacteria,understand and master the physiological and biochemical characteristics of strains.A theoretical foundation about how to train and screen oil bacterial strain could be formed. By measuring pH, surface tension, viscosity of crude oil and other indicators of crude oil emulsion formed in the process of strains and crude oil in the laboratory,we establish the single well basis in field test for the application of microbial stimulation.The main contents are as follows:Firstly, the development of MEOR as well as the research status in domestic and abroad were discussed, and the advantages of MEOR and the existing problems in the current and future development of MEOR technologies launched preconceptions were summarized. On this basis,This experiment lists the innovation points in this paper,and illustrate the research significance of this article clearly.Secondly, the separation of the oil displacement strains are screened. The oil was got as the only carbon source facultative celebrates the growth of bacterial strains 20 strains of oxygen, which come from daqing oilfield liquid, school corrupt oil pool, dining room oil pollution of soil and water, through the screen and purification at the beginning, the comprehensive growth ability to observe.Through analyzing the strains on the wave bottle drop oil, we screen four strains of bacteria namedâ… â…¡, A1 and A2, which could degrade oil well.Thirdly, These four oil strains above are identified. Programming a series of physiological and biochemical tests on the four strains screened out as to ensure they can produce acid, gas and biological surfactant which could extract oil more efficiently, Combined complicated physicochemical properties of trial with biochemical reaction experiment and 16S rDNA results, the strains are identified preliminarily as 2 kinds Bacillus subtilis, Pseudomonas aeruginosa and Bacillus cereus.Finally, the growth of 4 strains and the ability to degrade oil and decrease viscosity are further studied .According to the characteristics of target strains, the growth and the degradation of sticky oil were studied preliminarily.The result showed four strains have better ability to degrade oil and reduce oil viscosity.

Related Dissertations

  1. Flow Field Numerical Simulation and Performance Analysis for the Conical Hydrostatic Bearing,TH133.36
  2. Research on the Dynamics of Rotor-Cylindrical Bearing System and Rotor-Elloptical Bearing System,TK263.6
  3. Isolation and Identification of One Algae-Lysing Bacteria and the Lytic Effects on Two Blue-Green Algae in Prawn Pond,S942.91
  4. Comparison of Genetic Diversity of Total Planktonic Bacteria and Active Planktonic Bacteria in Huguangyan Maar Lake in Summer,Q938
  5. The Biodiesel Production from Wasted Edible Oil by Two Steps Catalyzed by Resin,TE667
  6. The Design and Performance Simulation of External Valve Small Displacement Pump,TE933.3
  7. Analysis of Gut Bacteria Diversity from Rice Planthoppers,S435.112.3
  8. Sinopec Sammy Quanzhou branch of marketing strategy research,F426.22
  9. Study on the System of Continous Process for Hydrogen Production by Photosynthetic Bacteria,TQ116.2
  10. Isolation Entophytes and Study on the Fermentation of Bacillus Subtilis tiD7 from Rehmannia,TQ461
  11. Biodiesel Production from Waste Oil and Study of Reduction of the Condensation Point,TE667
  12. The Detection of the Virulence-related Gene from Vibrio Alginolyticus and the Study on Preservation Methods,S943
  13. Preparation and Evaluation of Bio-Organic Fertilizers for Effective Control of Root-Knot Nematodes,S476.1
  14. Study on the Combined Probiotics and Its Effects on Prodution Performance of Pigs,S828.5
  15. The Effectr of Lactic Acid Bacteria and Rice Bran on Dynamic Change of Rice Straw Silage Fermentation,S816.53
  16. Isolation, Identification and Degradation Characteristics of a Benazolin-Ethyl Degrading Bacteria and Degradation Pathway,X172
  17. Red Soil Microbial Diversity of Peanut Continuous Cropping and Effects of Microorganisms on Growth of Continuous Cropping Peanut,S565.2
  18. Isolation of Siderophore Producting and Mineral Dissoluting Bacteria and the Interaction between Mica Minerals and Bacterias,Q93-33
  19. Study on Preparation of Biodiesel Catalyzed by Solid Base,TE667
  20. Treatment of High Concentration Oil-Bearing Wastewater in Food Industry with Membrane Biofilm Reactor (MBR),X792
  21. Combination Degradation of Chlor Pyrifos Via Biodegradation and Photodegradation,X592

CLC: > Industrial Technology > Oil and gas industry > Oil and gas development and mining > Production Engineering > Enhanced oil recovery and maintain reservoir pressure (secondary and tertiary oil recovery ) > Other methods
© 2012 www.DissertationTopic.Net  Mobile