Dissertation > Excellent graduate degree dissertation topics show

The Potential Mechanism of High Mobility Group Box 1 Protein on Meningioma Pathogenesis and Invasion

Author: DongYueQing
Tutor: YuXinGuang
School: PLA Postgraduate Medical School
Course: Surgery
Keywords: Meningiomas High mobility group box-1 protein MMP-9(matrix metalloproteinase-9) VEGF(vascular endothelial growth factor) Cdc-42 Apoptosis Cell cycle
CLC: R739.45
Type: PhD thesis
Year: 2008
Downloads: 80
Quote: 0
Read: Download Dissertation


Objective:High mobility group box 1 protein(HMGB1)is highly expressed in malignant cells,which is closely associated with tumor malignization and invasiveness.The present study was performed to determine the expression patterns of HMGB1 in meningiomas,and to clarify the potential mechanism of pathogensis and invasiveness of meningiomas,in turns to provide a new strategy for preventing and treating meningiomas.Methods:Fifty-six intracranial,totally resected benign and malignant meningiomas and ten normal brain tissuer were divided into there groups as follows:①in normal control group(n=10);②in benign group(WHOⅠgrade, n=50);③in malignant group(WHOⅢgrade,n=6).Samples of meningioma and normal brain tissue were harvested sterilely and divided into two parts:one part was detected expression of HMGB1,MMP-9,and VEGF by RT-PCR, western-blot and immunohisochemical analysis,and was determined activation of Cdc-42 with Western blot.The other part was prepared for cell culture.The cell proliferation was measured by MTT method,and cell apoptosis and cell cycle were observed with flow cytometry(FCM).Result:1.The expression of HMGB1 in meningiomas:(1)Compared with normal and benign group,the HMGB1 protein increased dramatically in malignant group(P<0.05 or P<0.01).However there was no significantly different between the normal group and benign group.(2)The mRNA expression of HMGB1 was significantly elevated in malignant group(P<0.01),compared with the normal and benign groups.There was a significant different between normal and benign group(P<0.05).(3)The expression of HMGB1 mRNA was coincident with that of HMGB1 protein in the malignant group.2.The effect of HMGB1 on meningioma invasiveness:(1)The mRNA expression of MPP-9 and VEGF was significantly elevated in malignant group(P<0.05 or P<0.01), compared with the normal and benign groups.(2)The protein amount of MMP-9 and VEGF identifyied by immunohisochemistry in malignant group was higher than normal and benign group(P<0.05),but there was no significant difference between the normal and benign groups.(3)The activation of Cdc-42 in malignant group was increased compared with normal and benign group(P<0.05).(4) There was significantly correlation between the mRNA expression of MMP-9 and VEGF and the HMGB1 in malignant group.3.Effect of HMGB1 on the apoptosis,cell proliferateion and cell cycle:(1)MTT measurement demonstrated that the malignant group had a higher cell proliferation and a more decreasing apoptosis rate than the benign group(P<0.05).(2)The G1 phase fraction(90%)in benign group was more than 10%in the malignant group (80%).The G2+S phase fraction(19.5%)in the malignant group was more than 8%in the benign group(11.7%).(3)The expression of HMGB1 mRNA was correlated with cell proliferation and apoptosis rate.Conclusions:1.The overexpression of HMGB1 may play a critical role in the pathogensis and invasiveness of meningiomas.2.The high expression of HMGB1 is correlated with the overexpression of MMP-9 and VEGF.HMGB1 may play an important role in the angiogenic and invasive processes in malignant meningiomas.3.HMGB1 may regulates cell migration and induce the invasive behaviour of malignant meningioma cell.4.HMGB1 may influence cell proliferateion and apoptosiss,and in turn induce pathogensis of meningiomas.

Related Dissertations

  1. Study of Oridonin on SGC-7901 Cell Proliferation by Inhibitting Cell Cycle Proteins,R285
  2. Expression of Prolyl Isomerase Pin1 in Osteosarcoma and the Effect of Regulation on Cell Cycle,R738.1
  3. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  4. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  5. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  6. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  7. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  8. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  9. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  10. Effects of Angiogenic Factor on Follicular Angiogenesis and Development in Sexual Maturity Mice,S852.2
  11. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  12. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  13. The Apoptosis Mechanism Induced by Aflatoxin B1 and Deoxynivalenol in Primary Hepatocyte of Cyprinus Carpio,S856.9
  14. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  15. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  16. The Effect of Foxol on Apoptosis of Mouse Granule Cells,S865.13
  17. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  18. The Protective Effect of Rat Myocardial Ischemia/reperfusion Injury Following Bone Marrow Mesenchymal Stem Cell Pretransplantation for 1 Week,R542.22
  19. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  20. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  21. Heme Oxygenase is Involved in Salinity-Induced Tomato Lateral Root Development and Its Relationship with Hydrogen Peroxide,S641.2

CLC: > Medicine, health > Oncology > Nervous system tumors > Meningeal tumor
© 2012 www.DissertationTopic.Net  Mobile